Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties
A novel exopolysaccharide (EPS-R1) from the Red Sea bacterium Bacillus tequilensis MYG163 was isolated and characterized. The strain produced 6.33 g/l of EPS-R1, which was identified as a sulfated heteropolysaccharide containing uronic acid, N-acetyl glucose amine, and various monosaccharides. EPS-R...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2024.2447151 |
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| author | Maha Abdullah Alwaili Mohammed A. Alshehri Thanaa R. Abdulrahman Faisal Miqad K. Albaqami Abdullah Alghamdi Mona Othman I. Albureikan Taghreed Shamrani Abdullah Albelasi Mohammad El-Nablaway Samy Selim Ahmed Ghareeb |
| author_facet | Maha Abdullah Alwaili Mohammed A. Alshehri Thanaa R. Abdulrahman Faisal Miqad K. Albaqami Abdullah Alghamdi Mona Othman I. Albureikan Taghreed Shamrani Abdullah Albelasi Mohammad El-Nablaway Samy Selim Ahmed Ghareeb |
| author_sort | Maha Abdullah Alwaili |
| collection | DOAJ |
| description | A novel exopolysaccharide (EPS-R1) from the Red Sea bacterium Bacillus tequilensis MYG163 was isolated and characterized. The strain produced 6.33 g/l of EPS-R1, which was identified as a sulfated heteropolysaccharide containing uronic acid, N-acetyl glucose amine, and various monosaccharides. EPS-R1 showed strong antioxidant properties (>80% in DPPH and ABTS assays) and anti-inflammatory effects on COX-1 (IC50 = 25.74 µg/mL) and COX-2 (IC50 = 48.57 µg/mL). While safe for normal cells, it demonstrated cytotoxicity against colon (IC50 = 203.25 µg/mL) and pancreatic cancer cells (IC50 = 122.99 µg/mL). The compound effectively inhibited Candida albicans (MIC = 15.62 µg/mL) and showed superior antibacterial activity compared to gentamicin against Enterococcus faecalis (33 mm vs. 30 mm) and Klebsiella pneumoniae (42 mm vs. 17 mm). It significantly reduced bacterial biofilm formation (86-88% inhibition at 75% MBC) and Candida biofilms (60-90% reduction). These diverse bioactivities suggest EPS-R1's potential as a therapeutic agent. |
| format | Article |
| id | doaj-art-37bcfd01b12f4f73b82f093b4a52c30e |
| institution | Kabale University |
| issn | 1658-3655 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-37bcfd01b12f4f73b82f093b4a52c30e2025-01-03T14:39:40ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552025-12-0119110.1080/16583655.2024.2447151Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm propertiesMaha Abdullah Alwaili0Mohammed A. Alshehri1Thanaa R. Abdulrahman2Faisal Miqad K. Albaqami3Abdullah Alghamdi4Mona Othman I. Albureikan5Taghreed Shamrani6Abdullah Albelasi7Mohammad El-Nablaway8Samy Selim9Ahmed Ghareeb10Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Microbiology, College of Medicine, Al-Nahrain University, Al-Kadhimiya, IraqBiology Department, Faculty of Science, Islamic University of Madinah, Madinah, Saudi ArabiaDepartment of Medical Laboratory, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Medical Laboratories, College of Applied Medical Sciences, Shaqra University, Shaqra, Saudi ArabiaDepartment of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia, EgyptA novel exopolysaccharide (EPS-R1) from the Red Sea bacterium Bacillus tequilensis MYG163 was isolated and characterized. The strain produced 6.33 g/l of EPS-R1, which was identified as a sulfated heteropolysaccharide containing uronic acid, N-acetyl glucose amine, and various monosaccharides. EPS-R1 showed strong antioxidant properties (>80% in DPPH and ABTS assays) and anti-inflammatory effects on COX-1 (IC50 = 25.74 µg/mL) and COX-2 (IC50 = 48.57 µg/mL). While safe for normal cells, it demonstrated cytotoxicity against colon (IC50 = 203.25 µg/mL) and pancreatic cancer cells (IC50 = 122.99 µg/mL). The compound effectively inhibited Candida albicans (MIC = 15.62 µg/mL) and showed superior antibacterial activity compared to gentamicin against Enterococcus faecalis (33 mm vs. 30 mm) and Klebsiella pneumoniae (42 mm vs. 17 mm). It significantly reduced bacterial biofilm formation (86-88% inhibition at 75% MBC) and Candida biofilms (60-90% reduction). These diverse bioactivities suggest EPS-R1's potential as a therapeutic agent.https://www.tandfonline.com/doi/10.1080/16583655.2024.2447151Microbial metabolitesnatural productsdrug discoveryantimicrobial resistanceanti-candidiasisantifungal |
| spellingShingle | Maha Abdullah Alwaili Mohammed A. Alshehri Thanaa R. Abdulrahman Faisal Miqad K. Albaqami Abdullah Alghamdi Mona Othman I. Albureikan Taghreed Shamrani Abdullah Albelasi Mohammad El-Nablaway Samy Selim Ahmed Ghareeb Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties Journal of Taibah University for Science Microbial metabolites natural products drug discovery antimicrobial resistance anti-candidiasis antifungal |
| title | Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties |
| title_full | Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties |
| title_fullStr | Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties |
| title_full_unstemmed | Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties |
| title_short | Broad-spectrum bioactivities of a sulfated heteropolysaccharide from Bacillus tequilensis MYG163: antioxidant, anti-Inflammatory, anticancer, antimicrobial, and antibiofilm properties |
| title_sort | broad spectrum bioactivities of a sulfated heteropolysaccharide from bacillus tequilensis myg163 antioxidant anti inflammatory anticancer antimicrobial and antibiofilm properties |
| topic | Microbial metabolites natural products drug discovery antimicrobial resistance anti-candidiasis antifungal |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2024.2447151 |
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