Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage
In order to explore the effects of additives on the chemical composition, fermentation characteristics, and bacterial community of <i>Pennisetum giganteum</i> z.x.lin silage, <i>Pennisetum giganteum</i> z.x.lin was ensiled with no additives (CON), cellulase (CE), <i>Lac...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/15/1/97 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841549492545912832 |
|---|---|
| author | Zhili Wu Xiongfei Zhang Rongnuo Li Jingtao Hui Lu Deng Inho Kim Jie Wei Junhu Yao Xinjian Lei |
| author_facet | Zhili Wu Xiongfei Zhang Rongnuo Li Jingtao Hui Lu Deng Inho Kim Jie Wei Junhu Yao Xinjian Lei |
| author_sort | Zhili Wu |
| collection | DOAJ |
| description | In order to explore the effects of additives on the chemical composition, fermentation characteristics, and bacterial community of <i>Pennisetum giganteum</i> z.x.lin silage, <i>Pennisetum giganteum</i> z.x.lin was ensiled with no additives (CON), cellulase (CE), <i>Lactiplantibacillus plantarum</i> (LP), or the combination of cellulase and <i>Lactiplantibacillus plantarum</i> (LPCE) at room temperature for 60 days, respectively. The results indicated that LPCE had the highest dry matter (DM) content. Compared with CON, LP exhibited higher (<i>p</i> < 0.05) levels of water-soluble carbohydrate (WSC), crude protein (CP), and lactic acid (LA), along with a higher (<i>p</i> < 0.05) ratio of LA/acetic acid (AA). Meanwhile, silage inoculated with cellulase (CE and LPCE) showed lower (<i>p</i> < 0.05) contents of acid detergent fiber (ADF) and neutral detergent fiber (NDF) than CON. Furthermore, additive treatments improved the bacterial community composition of silage, and <i>Lactobacillus</i> was abundant in LPCE (LDA score > 4.0). Compared with CE and LP, LPCE more effectively promoted the transformation of microbial functions, resulting in an upregulated (<i>p</i> < 0.05) carbohydrate metabolism and a downregulated (<i>p</i> < 0.05) membrane transport. In conclusion, cellulase or <i>Lactiplantibacillus plantarum</i> improved the silage quality of <i>Pennisetum giganteum</i> z.x.lin by reducing the fiber content or enhancing LA fermentation, and their combination exhibited a powerful ability to establish a bacterial community dominated by <i>Lactobacillus</i>, which facilitated the production of high-quality silage. |
| format | Article |
| id | doaj-art-37865c65d2764755bb8d6765382958f4 |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-37865c65d2764755bb8d6765382958f42025-01-10T13:13:41ZengMDPI AGAgriculture2077-04722025-01-011519710.3390/agriculture15010097Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin SilageZhili Wu0Xiongfei Zhang1Rongnuo Li2Jingtao Hui3Lu Deng4Inho Kim5Jie Wei6Junhu Yao7Xinjian Lei8College of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaDepartment of Animal Resource and Science, Dankook University, Cheonan 31116, Republic of KoreaXi’an Architectural Design and Research Institute Co., Ltd., Xi’an 710000, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Xianyang 712100, ChinaIn order to explore the effects of additives on the chemical composition, fermentation characteristics, and bacterial community of <i>Pennisetum giganteum</i> z.x.lin silage, <i>Pennisetum giganteum</i> z.x.lin was ensiled with no additives (CON), cellulase (CE), <i>Lactiplantibacillus plantarum</i> (LP), or the combination of cellulase and <i>Lactiplantibacillus plantarum</i> (LPCE) at room temperature for 60 days, respectively. The results indicated that LPCE had the highest dry matter (DM) content. Compared with CON, LP exhibited higher (<i>p</i> < 0.05) levels of water-soluble carbohydrate (WSC), crude protein (CP), and lactic acid (LA), along with a higher (<i>p</i> < 0.05) ratio of LA/acetic acid (AA). Meanwhile, silage inoculated with cellulase (CE and LPCE) showed lower (<i>p</i> < 0.05) contents of acid detergent fiber (ADF) and neutral detergent fiber (NDF) than CON. Furthermore, additive treatments improved the bacterial community composition of silage, and <i>Lactobacillus</i> was abundant in LPCE (LDA score > 4.0). Compared with CE and LP, LPCE more effectively promoted the transformation of microbial functions, resulting in an upregulated (<i>p</i> < 0.05) carbohydrate metabolism and a downregulated (<i>p</i> < 0.05) membrane transport. In conclusion, cellulase or <i>Lactiplantibacillus plantarum</i> improved the silage quality of <i>Pennisetum giganteum</i> z.x.lin by reducing the fiber content or enhancing LA fermentation, and their combination exhibited a powerful ability to establish a bacterial community dominated by <i>Lactobacillus</i>, which facilitated the production of high-quality silage.https://www.mdpi.com/2077-0472/15/1/97bio-additivesensilingenzyme-bacteria synergymicrobial diversity<i>Pennisetum giganteum</i> z.x.lin |
| spellingShingle | Zhili Wu Xiongfei Zhang Rongnuo Li Jingtao Hui Lu Deng Inho Kim Jie Wei Junhu Yao Xinjian Lei Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage Agriculture bio-additives ensiling enzyme-bacteria synergy microbial diversity <i>Pennisetum giganteum</i> z.x.lin |
| title | Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage |
| title_full | Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage |
| title_fullStr | Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage |
| title_full_unstemmed | Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage |
| title_short | Effects of Cellulase and <i>Lactiplantibacillus plantarum</i> on Chemical Composition, Fermentation Characteristics, and Bacterial Community of <i>Pennisetum giganteum</i> z.x.lin Silage |
| title_sort | effects of cellulase and i lactiplantibacillus plantarum i on chemical composition fermentation characteristics and bacterial community of i pennisetum giganteum i z x lin silage |
| topic | bio-additives ensiling enzyme-bacteria synergy microbial diversity <i>Pennisetum giganteum</i> z.x.lin |
| url | https://www.mdpi.com/2077-0472/15/1/97 |
| work_keys_str_mv | AT zhiliwu effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT xiongfeizhang effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT rongnuoli effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT jingtaohui effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT ludeng effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT inhokim effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT jiewei effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT junhuyao effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage AT xinjianlei effectsofcellulaseandilactiplantibacillusplantarumionchemicalcompositionfermentationcharacteristicsandbacterialcommunityofipennisetumgiganteumizxlinsilage |