Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli
Abstract Objectives The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophag...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-024-07033-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559872569606144 |
|---|---|
| author | Rania Abozahra Dina Shlkamy Sarah M. Abdelhamid |
| author_facet | Rania Abozahra Dina Shlkamy Sarah M. Abdelhamid |
| author_sort | Rania Abozahra |
| collection | DOAJ |
| description | Abstract Objectives The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination. Results ɸEcM-vB1 bacteriophage exhibited high lytic activity against different MDR E. coli isolates and showed stability over wide pH and temperature range. It belongs to the Myoviridae family of the caudovirales order according to TEM. It had a latent period of 5 min and an average burst size of 271.72 pfu/cell. Genomic analysis revealed that it is susceptible to digestion by EcoRI. Ten structural proteins were detected by SDS-PAGE. ɸEcM-vB1 is considered a promising candidate for phage therapy applications. |
| format | Article |
| id | doaj-art-377e38f05bc84a26b2ceb4dd54862761 |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-377e38f05bc84a26b2ceb4dd548627612025-01-05T12:07:40ZengBMCBMC Research Notes1756-05002025-01-0118111010.1186/s13104-024-07033-xIsolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coliRania Abozahra0Dina Shlkamy1Sarah M. Abdelhamid2Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Damanhour UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Damanhour UniversityAbstract Objectives The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination. Results ɸEcM-vB1 bacteriophage exhibited high lytic activity against different MDR E. coli isolates and showed stability over wide pH and temperature range. It belongs to the Myoviridae family of the caudovirales order according to TEM. It had a latent period of 5 min and an average burst size of 271.72 pfu/cell. Genomic analysis revealed that it is susceptible to digestion by EcoRI. Ten structural proteins were detected by SDS-PAGE. ɸEcM-vB1 is considered a promising candidate for phage therapy applications.https://doi.org/10.1186/s13104-024-07033-xBacteriophageMultidrug resistanceEscherichia coli |
| spellingShingle | Rania Abozahra Dina Shlkamy Sarah M. Abdelhamid Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli BMC Research Notes Bacteriophage Multidrug resistance Escherichia coli |
| title | Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli |
| title_full | Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli |
| title_fullStr | Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli |
| title_full_unstemmed | Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli |
| title_short | Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli |
| title_sort | isolation and characterization of ɸecm vb1 bacteriophage targeting multidrug resistant escherichia coli |
| topic | Bacteriophage Multidrug resistance Escherichia coli |
| url | https://doi.org/10.1186/s13104-024-07033-x |
| work_keys_str_mv | AT raniaabozahra isolationandcharacterizationofɸecmvb1bacteriophagetargetingmultidrugresistantescherichiacoli AT dinashlkamy isolationandcharacterizationofɸecmvb1bacteriophagetargetingmultidrugresistantescherichiacoli AT sarahmabdelhamid isolationandcharacterizationofɸecmvb1bacteriophagetargetingmultidrugresistantescherichiacoli |