Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue
Photoacoustic imaging (PAI) is a developing image modality that benefits from light–matter interaction and low acoustic attenuation to provide functional information on tissue composition at relatively large depths. Several studies have reported the potential of dichroism-sensitive photoacoustic (DS...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597924000934 |
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| author | Camilo Cano Amir Gholampour Marc van Sambeek Richard Lopata Min Wu |
| author_facet | Camilo Cano Amir Gholampour Marc van Sambeek Richard Lopata Min Wu |
| author_sort | Camilo Cano |
| collection | DOAJ |
| description | Photoacoustic imaging (PAI) is a developing image modality that benefits from light–matter interaction and low acoustic attenuation to provide functional information on tissue composition at relatively large depths. Several studies have reported the potential of dichroism-sensitive photoacoustic (DS-PA) imaging to expand PAI capabilities by obtaining morphological information of tissue regarding anisotropy and predominant orientation. However, most of these studies have limited their analysis to superficial scanning of samples, where fluence effects are negligible. Herein, we present a mathematical model for the in-depth analysis of the DS-PA signal of biological samples, focusing on estimating tissue orientation. Our model is validated with a B-scan setup for DS-PA imaging in ex-vivo porcine tendon samples, for which collagen displays optical anisotropy. Results show that for in-depth DS-PA imaging, the accumulative fluence modulation due to dichroism overcomes the effect of absorption dichroism affecting the measured signals; however, this effect can be corrected based on the presented model for determining fiber orientation. |
| format | Article |
| id | doaj-art-36e6984417b14416a5b54ef5ebafb91b |
| institution | Kabale University |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-36e6984417b14416a5b54ef5ebafb91b2025-01-17T04:49:31ZengElsevierPhotoacoustics2213-59792025-02-0141100676Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissueCamilo Cano0Amir Gholampour1Marc van Sambeek2Richard Lopata3Min Wu4Department of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The Netherlands; Corresponding author.Department of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The NetherlandsDepartment of Vascular Surgery, Catharina Ziekenhuis Eindhoven, Michelangelolaan 2, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The NetherlandsDepartment of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The NetherlandsDepartment of Biomedical Engineering, Eindhoven University of Technology, De Rondom 70, Eindhoven, The NetherlandsPhotoacoustic imaging (PAI) is a developing image modality that benefits from light–matter interaction and low acoustic attenuation to provide functional information on tissue composition at relatively large depths. Several studies have reported the potential of dichroism-sensitive photoacoustic (DS-PA) imaging to expand PAI capabilities by obtaining morphological information of tissue regarding anisotropy and predominant orientation. However, most of these studies have limited their analysis to superficial scanning of samples, where fluence effects are negligible. Herein, we present a mathematical model for the in-depth analysis of the DS-PA signal of biological samples, focusing on estimating tissue orientation. Our model is validated with a B-scan setup for DS-PA imaging in ex-vivo porcine tendon samples, for which collagen displays optical anisotropy. Results show that for in-depth DS-PA imaging, the accumulative fluence modulation due to dichroism overcomes the effect of absorption dichroism affecting the measured signals; however, this effect can be corrected based on the presented model for determining fiber orientation.http://www.sciencedirect.com/science/article/pii/S2213597924000934Photoacoustic imagingDichroism imagingAnisotropic tissueOptic axis estimation |
| spellingShingle | Camilo Cano Amir Gholampour Marc van Sambeek Richard Lopata Min Wu Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue Photoacoustics Photoacoustic imaging Dichroism imaging Anisotropic tissue Optic axis estimation |
| title | Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue |
| title_full | Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue |
| title_fullStr | Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue |
| title_full_unstemmed | Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue |
| title_short | Dichroism-sensitive photoacoustic imaging for in-depth estimation of the optic axis in fibrous tissue |
| title_sort | dichroism sensitive photoacoustic imaging for in depth estimation of the optic axis in fibrous tissue |
| topic | Photoacoustic imaging Dichroism imaging Anisotropic tissue Optic axis estimation |
| url | http://www.sciencedirect.com/science/article/pii/S2213597924000934 |
| work_keys_str_mv | AT camilocano dichroismsensitivephotoacousticimagingforindepthestimationoftheopticaxisinfibroustissue AT amirgholampour dichroismsensitivephotoacousticimagingforindepthestimationoftheopticaxisinfibroustissue AT marcvansambeek dichroismsensitivephotoacousticimagingforindepthestimationoftheopticaxisinfibroustissue AT richardlopata dichroismsensitivephotoacousticimagingforindepthestimationoftheopticaxisinfibroustissue AT minwu dichroismsensitivephotoacousticimagingforindepthestimationoftheopticaxisinfibroustissue |