An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals

Introduction: Bacteria play a key role in pulp and periapical disease, making their complete removal essential in endodontic treatment. Calcium hydroxide (Ca(OH)₂) is commonly used as an intracanal medicament due to its antimicrobial properties, but its remnants can hinder sealer adhesion and affect...

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Main Authors: Manjula Srinivas Pandurangan, Bandlapally Sreenivasa Guptha Anila, Roopadevi Garlapati, Niharika Halder, Mayana Aameena Banu, Lavanya Modala
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2024-07-01
Series:Journal of Orofacial Sciences
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Online Access:https://journals.lww.com/10.4103/jofs.jofs_237_23
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author Manjula Srinivas Pandurangan
Bandlapally Sreenivasa Guptha Anila
Roopadevi Garlapati
Niharika Halder
Mayana Aameena Banu
Lavanya Modala
author_facet Manjula Srinivas Pandurangan
Bandlapally Sreenivasa Guptha Anila
Roopadevi Garlapati
Niharika Halder
Mayana Aameena Banu
Lavanya Modala
author_sort Manjula Srinivas Pandurangan
collection DOAJ
description Introduction: Bacteria play a key role in pulp and periapical disease, making their complete removal essential in endodontic treatment. Calcium hydroxide (Ca(OH)₂) is commonly used as an intracanal medicament due to its antimicrobial properties, but its remnants can hinder sealer adhesion and affect treatment outcomes. This study evaluated the effectiveness of 0.5% peracetic acid (PAA), 10% citric acid (CA), 1% PAA, 3% sodium hypochlorite (NaOCl), and 17% ethylenediamine tetraacetic acid (EDTA) with passive ultrasonic irrigation (PUI), in removing Ca(OH)₂ from root canals. Materials and Method: Sixty teeth that had single roots were chosen and decoronated to set the length of the root at 12 mm. Canals were irrigated, followed by filling up with Ca(OH)₂ after biomechanical preparation. The samples were divided into seven groups: Group 1: Control (positive); Group 2: Control (negative); Group 3: 5 ml of 17% EDTA + PUI; Group 4: 5 ml of 3 % NaOCl+ PUI; Group 5: 5 ml of 0.5% PAA + PUI; Group 6: 5 ml of 1% PAA + PUI; Group 7: 10% CA + PUI. The radicular portions of teeth were split longitudinally, followed by examination beneath a stereomicroscope with a magnification of 50×. Results: The obtained data was statistically analyzed using the Kruskal–Wallis test to find out significant differences between the study groups. Pair-wise comparison for mean scores was done using the Mann–Whitney test. Friedman’s test was used for intra group differences between regions. Small amounts of Ca(OH)₂ residue were observed in the 1% PAA group compared to other irrigants in the coronal, middle, and apical thirds of the root canals, and the difference was statistically significant (p < 0.05). Conclusion: About 1% PAA with PUI was superior in removing Ca(OH)₂ from root canals compared to other irrigants used in the present study.
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spelling doaj-art-36c07c3802ee428591c9df7d005b89ea2025-01-04T15:11:28ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442024-07-0116213914510.4103/jofs.jofs_237_23An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root CanalsManjula Srinivas PanduranganBandlapally Sreenivasa Guptha AnilaRoopadevi GarlapatiNiharika HalderMayana Aameena BanuLavanya ModalaIntroduction: Bacteria play a key role in pulp and periapical disease, making their complete removal essential in endodontic treatment. Calcium hydroxide (Ca(OH)₂) is commonly used as an intracanal medicament due to its antimicrobial properties, but its remnants can hinder sealer adhesion and affect treatment outcomes. This study evaluated the effectiveness of 0.5% peracetic acid (PAA), 10% citric acid (CA), 1% PAA, 3% sodium hypochlorite (NaOCl), and 17% ethylenediamine tetraacetic acid (EDTA) with passive ultrasonic irrigation (PUI), in removing Ca(OH)₂ from root canals. Materials and Method: Sixty teeth that had single roots were chosen and decoronated to set the length of the root at 12 mm. Canals were irrigated, followed by filling up with Ca(OH)₂ after biomechanical preparation. The samples were divided into seven groups: Group 1: Control (positive); Group 2: Control (negative); Group 3: 5 ml of 17% EDTA + PUI; Group 4: 5 ml of 3 % NaOCl+ PUI; Group 5: 5 ml of 0.5% PAA + PUI; Group 6: 5 ml of 1% PAA + PUI; Group 7: 10% CA + PUI. The radicular portions of teeth were split longitudinally, followed by examination beneath a stereomicroscope with a magnification of 50×. Results: The obtained data was statistically analyzed using the Kruskal–Wallis test to find out significant differences between the study groups. Pair-wise comparison for mean scores was done using the Mann–Whitney test. Friedman’s test was used for intra group differences between regions. Small amounts of Ca(OH)₂ residue were observed in the 1% PAA group compared to other irrigants in the coronal, middle, and apical thirds of the root canals, and the difference was statistically significant (p < 0.05). Conclusion: About 1% PAA with PUI was superior in removing Ca(OH)₂ from root canals compared to other irrigants used in the present study.https://journals.lww.com/10.4103/jofs.jofs_237_23peracetic acidedtacitric acidpuistereomicroscope
spellingShingle Manjula Srinivas Pandurangan
Bandlapally Sreenivasa Guptha Anila
Roopadevi Garlapati
Niharika Halder
Mayana Aameena Banu
Lavanya Modala
An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
Journal of Orofacial Sciences
peracetic acid
edta
citric acid
pui
stereomicroscope
title An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
title_full An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
title_fullStr An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
title_full_unstemmed An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
title_short An In-Vitro Comparison of Effects of Passive Ultrasonic Irrigation with Various Concentrations of Peracetic Acid, Citric Acid, Sodium Hypochlorite, and Ethylene Diamine Tetra Acetic Acid on Removal of Calcium Hydroxide from Root Canals
title_sort in vitro comparison of effects of passive ultrasonic irrigation with various concentrations of peracetic acid citric acid sodium hypochlorite and ethylene diamine tetra acetic acid on removal of calcium hydroxide from root canals
topic peracetic acid
edta
citric acid
pui
stereomicroscope
url https://journals.lww.com/10.4103/jofs.jofs_237_23
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