In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals
Extracellular vesicles derived from human umbilical cord-derived mesenchymal stem cells (UCMSC-EVs) have been postulated to have therapeutic potential for various diseases. However, the biodistribution and pharmacokinetics of these vesicles are still unclear. For a better understanding of the in viv...
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Elsevier
2024-10-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2319417024000246 |
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| author | Yi-Hsiu Chung Yi-Pei Ho Shiou-Shiow Farn Wei-Cheng Tsai Zhi-Xiang Li Tzou-Yien Lin Chi-Chang Weng |
| author_facet | Yi-Hsiu Chung Yi-Pei Ho Shiou-Shiow Farn Wei-Cheng Tsai Zhi-Xiang Li Tzou-Yien Lin Chi-Chang Weng |
| author_sort | Yi-Hsiu Chung |
| collection | DOAJ |
| description | Extracellular vesicles derived from human umbilical cord-derived mesenchymal stem cells (UCMSC-EVs) have been postulated to have therapeutic potential for various diseases. However, the biodistribution and pharmacokinetics of these vesicles are still unclear. For a better understanding of the in vivo properties of UCMSC-EVs, in the present study, these vesicles were first radiolabeled with Technetium-99m (99mTc-UCMSC-EVs) and evaluated using in vivo single photon emission computed tomography (SPECT) imaging and biodistribution experiments. SPECT images demonstrated that the liver and spleen tissues mainly took up the 99mTc-UCMSC-EVs. The biodistribution study observed slight uptake in the thyroid and stomach, indicating that 99mTc-UCMSC-EVs was stable at 24 h in vivo. The pharmacokinetic analyses of the blood half-life demonstrated the quick distribution phase (0.85 ± 0.28 min) and elimination phase (25.22 ± 20.76 min) in mice. This study provides a convenient and efficient method for 99mTc-UCMSC-EVs preparation without disturbing their properties. In conclusion, the biodistribution, quick elimination, and suitable stability in vivo of 99mTc-UCMSC-EVs were quantified by the noninvasive imaging and pharmacokinetic analyses, which provides useful information for indication selection, dosage protocol design, and toxicity assessment in future applications. |
| format | Article |
| id | doaj-art-36a5aa49e2f34c42b77a8e407cdceb0a |
| institution | Kabale University |
| issn | 2319-4170 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
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| series | Biomedical Journal |
| spelling | doaj-art-36a5aa49e2f34c42b77a8e407cdceb0a2024-11-20T05:07:15ZengElsevierBiomedical Journal2319-41702024-10-01475100721In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animalsYi-Hsiu Chung0Yi-Pei Ho1Shiou-Shiow Farn2Wei-Cheng Tsai3Zhi-Xiang Li4Tzou-Yien Lin5Chi-Chang Weng6Department of Medical Research and Development, Research Division, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanDepartment of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan, Taiwan; Healthy Aging Research Center, Chang GungUniversity, Taoyuan, TaiwanDepartment of Isotope Application Research, National Atomic Research Institute, Taoyuan, TaiwanExoOne Bio Co., Ltd., Taipei, TaiwanExoOne Bio Co., Ltd., Taipei, TaiwanDepartment of Pediatrics, Division of Infectious Diseases, Chang Gung Memorial Hospital, Taoyuan, Taiwan; College of Medicine, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Department of Pediatrics, Division of Infectious Diseases, Chang Gung Memorial Hospital, No. 5, Fuxing St., Guishan Dist., Taoyuan City 33305, Taiwan.Department of Medical Research and Development, Research Division, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Department of Medical Imaging and Radiological Sciences, Chang Gung University, Taoyuan, Taiwan; Healthy Aging Research Center, Chang GungUniversity, Taoyuan, Taiwan; Corresponding author. Department of Medical Imaging and Radiological Sciences, Chang Gung University, No. 259, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33302, Taiwan.Extracellular vesicles derived from human umbilical cord-derived mesenchymal stem cells (UCMSC-EVs) have been postulated to have therapeutic potential for various diseases. However, the biodistribution and pharmacokinetics of these vesicles are still unclear. For a better understanding of the in vivo properties of UCMSC-EVs, in the present study, these vesicles were first radiolabeled with Technetium-99m (99mTc-UCMSC-EVs) and evaluated using in vivo single photon emission computed tomography (SPECT) imaging and biodistribution experiments. SPECT images demonstrated that the liver and spleen tissues mainly took up the 99mTc-UCMSC-EVs. The biodistribution study observed slight uptake in the thyroid and stomach, indicating that 99mTc-UCMSC-EVs was stable at 24 h in vivo. The pharmacokinetic analyses of the blood half-life demonstrated the quick distribution phase (0.85 ± 0.28 min) and elimination phase (25.22 ± 20.76 min) in mice. This study provides a convenient and efficient method for 99mTc-UCMSC-EVs preparation without disturbing their properties. In conclusion, the biodistribution, quick elimination, and suitable stability in vivo of 99mTc-UCMSC-EVs were quantified by the noninvasive imaging and pharmacokinetic analyses, which provides useful information for indication selection, dosage protocol design, and toxicity assessment in future applications.http://www.sciencedirect.com/science/article/pii/S2319417024000246Tc-99mUCMSC-EVsSPECTBiodistributionPharmacokinetic analyses |
| spellingShingle | Yi-Hsiu Chung Yi-Pei Ho Shiou-Shiow Farn Wei-Cheng Tsai Zhi-Xiang Li Tzou-Yien Lin Chi-Chang Weng In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals Biomedical Journal Tc-99m UCMSC-EVs SPECT Biodistribution Pharmacokinetic analyses |
| title | In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals |
| title_full | In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals |
| title_fullStr | In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals |
| title_full_unstemmed | In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals |
| title_short | In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals |
| title_sort | in vivo spect imaging of tc 99m radiolabeled exosomes from human umbilical cord derived mesenchymal stem cells in small animals |
| topic | Tc-99m UCMSC-EVs SPECT Biodistribution Pharmacokinetic analyses |
| url | http://www.sciencedirect.com/science/article/pii/S2319417024000246 |
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