Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study
Aim To determine the antimicrobial activity of the bacteriocin-producing probiotic strains Streptococcus salivarius K12 and Streptococcus salivarius M18 alone or in combination against caries-associated Streptococcus mutans.Methods Antimicrobial activity of S. salivarius K12 and/or S. salivarius M18...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2304971 |
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| author | Elisabeth Reichardt Viktoriya Shyp Lea Alig Carlalberta Verna Eva M. Kulik Michael M. Bornstein |
| author_facet | Elisabeth Reichardt Viktoriya Shyp Lea Alig Carlalberta Verna Eva M. Kulik Michael M. Bornstein |
| author_sort | Elisabeth Reichardt |
| collection | DOAJ |
| description | Aim To determine the antimicrobial activity of the bacteriocin-producing probiotic strains Streptococcus salivarius K12 and Streptococcus salivarius M18 alone or in combination against caries-associated Streptococcus mutans.Methods Antimicrobial activity of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 growth and biofilm formation on hydroxyapatite (HA) discs was determined in a flow chamber model by recording the colony forming units (CFU/ml) after 48 h of co-cultivation. The biofilm was analyzed by scanning electron microscopy (SEM) and by confocal laser scanning microscopy (CLSM). Additionally, the simultaneous antagonism assay was used to assess the inhibitory effect of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 and 21 clinical isolates of S. mutans.Results Co-cultivation of S. mutans and S. salivarius K12 and/or S. salivarius M18 led to the inhibition of S. mutans viability, thereby, preventing its biofilm formation on HA discs. Furthermore, S. salivarius K12 and S. salivarius M18 exhibited antimicrobial activity against most clinical isolates of S. mutans.Conclusion The in vitro flow chamber system used in this study allows the simulation of time-dependent administration of S. salivarius probiotic strains, either alone or in combination, to investigate the prevention of S. mutans biofilm formation in a standardized model. |
| format | Article |
| id | doaj-art-367c20fdad91455c82393ee0380f4c66 |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-367c20fdad91455c82393ee0380f4c662024-11-26T06:52:12ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2024.2304971Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro studyElisabeth Reichardt0Viktoriya Shyp1Lea Alig2Carlalberta Verna3Eva M. Kulik4Michael M. Bornstein5Department of Pediatric Oral Health and Orthodontics, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandDepartment Research, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandDepartment Research, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandDepartment of Pediatric Oral Health and Orthodontics, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandDepartment Research, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandDepartment of Oral Health & Medicine, University Center for Dental Medicine UZB, University of Basel, Basel, SwitzerlandAim To determine the antimicrobial activity of the bacteriocin-producing probiotic strains Streptococcus salivarius K12 and Streptococcus salivarius M18 alone or in combination against caries-associated Streptococcus mutans.Methods Antimicrobial activity of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 growth and biofilm formation on hydroxyapatite (HA) discs was determined in a flow chamber model by recording the colony forming units (CFU/ml) after 48 h of co-cultivation. The biofilm was analyzed by scanning electron microscopy (SEM) and by confocal laser scanning microscopy (CLSM). Additionally, the simultaneous antagonism assay was used to assess the inhibitory effect of S. salivarius K12 and/or S. salivarius M18 against S. mutans ATCC 25175 and 21 clinical isolates of S. mutans.Results Co-cultivation of S. mutans and S. salivarius K12 and/or S. salivarius M18 led to the inhibition of S. mutans viability, thereby, preventing its biofilm formation on HA discs. Furthermore, S. salivarius K12 and S. salivarius M18 exhibited antimicrobial activity against most clinical isolates of S. mutans.Conclusion The in vitro flow chamber system used in this study allows the simulation of time-dependent administration of S. salivarius probiotic strains, either alone or in combination, to investigate the prevention of S. mutans biofilm formation in a standardized model.https://www.tandfonline.com/doi/10.1080/20002297.2024.2304971Probioticsbiofilmantibacterial therapyStreptococcus salivarius K12bacteriocin-like inhibitory substancesStreptococcus salivarius M18 |
| spellingShingle | Elisabeth Reichardt Viktoriya Shyp Lea Alig Carlalberta Verna Eva M. Kulik Michael M. Bornstein Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study Journal of Oral Microbiology Probiotics biofilm antibacterial therapy Streptococcus salivarius K12 bacteriocin-like inhibitory substances Streptococcus salivarius M18 |
| title | Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study |
| title_full | Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study |
| title_fullStr | Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study |
| title_full_unstemmed | Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study |
| title_short | Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study |
| title_sort | antimicrobial effect of probiotic bacteriocins on streptococcus mutans biofilm in a dynamic oral flow chamber model an in vitro study |
| topic | Probiotics biofilm antibacterial therapy Streptococcus salivarius K12 bacteriocin-like inhibitory substances Streptococcus salivarius M18 |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2304971 |
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