Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV
Japanese encephalitis virus (JEV) genotype IV (GIV) is one of the least common and most neglected genotypes worldwide, having been identified only on a few Indonesian islands until it was recently found to be the cause of outbreaks that occurred in several Australian states in early 2022. Given the...
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2024.2438661 |
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| author | Astri Nur Faizah Daisuke Kobayashi Faustus Akankperiwen Azerigyik Ryo Matsumura Izumi Kai Yoshihide Maekawa Yukiko Higa Kentaro Itokawa Toshinori Sasaki Kris Cahyo Mulyatno Sri Subekti Maria Inge Lusida Etik Ainun Rohmah Yasuko Mori Yusuf Ozbel Chizu Sanjoba Tran Vu Phong Tran Cong Tu Shinji Kasai Kyoko Sawabe Haruhiko Isawa |
| author_facet | Astri Nur Faizah Daisuke Kobayashi Faustus Akankperiwen Azerigyik Ryo Matsumura Izumi Kai Yoshihide Maekawa Yukiko Higa Kentaro Itokawa Toshinori Sasaki Kris Cahyo Mulyatno Sri Subekti Maria Inge Lusida Etik Ainun Rohmah Yasuko Mori Yusuf Ozbel Chizu Sanjoba Tran Vu Phong Tran Cong Tu Shinji Kasai Kyoko Sawabe Haruhiko Isawa |
| author_sort | Astri Nur Faizah |
| collection | DOAJ |
| description | Japanese encephalitis virus (JEV) genotype IV (GIV) is one of the least common and most neglected genotypes worldwide, having been identified only on a few Indonesian islands until it was recently found to be the cause of outbreaks that occurred in several Australian states in early 2022. Given the limited availability of information, the vector range for JEV GIV remains unknown; thus, understanding this range could prove invaluable for future prevention efforts in new areas. Herein, we experimentally exposed four mosquito colonies originated from various countries with no previous reports of GIV to JEV GIV strain 19CxBa-83-Cv, which was isolated from Culex vishnui Theobald collected in Bali in 2019. At 7 and 14 days post-JEV GIV exposure through a membrane feeding method, mosquito bodies, head–wings–legs, and saliva were harvested for infection, dissemination, and transmission efficiency analyses. The results showed robust transmission efficiencies of the virus by Culex tritaeniorhynchus Giles (∼74%) and Aedes albopictus Skuse (∼52%) from Japan, followed by Culex quinquefasciatus Say from Vietnam (∼35%) and Culex pipiens form molestus from Turkey (∼18%). Although significant differences were observed, we found that the four mosquito species could transmit JEV GIV. The efficiency of biological transmission of this restricted genotype by mosquitoes from various origins suggests that these mosquito species could support localized transmission if the genotype were introduced to their respective areas. This study emphasizes the importance of remaining vigilant and continuing arbovirus surveillance in all locations. |
| format | Article |
| id | doaj-art-36226f2b95654812a1d02be61e492590 |
| institution | Kabale University |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-36226f2b95654812a1d02be61e4925902025-01-03T06:05:48ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2024.2438661Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IVAstri Nur Faizah0Daisuke Kobayashi1Faustus Akankperiwen Azerigyik2Ryo Matsumura3Izumi Kai4Yoshihide Maekawa5Yukiko Higa6Kentaro Itokawa7Toshinori Sasaki8Kris Cahyo Mulyatno9Sri Subekti10Maria Inge Lusida11Etik Ainun Rohmah12Yasuko Mori13Yusuf Ozbel14Chizu Sanjoba15Tran Vu Phong16Tran Cong Tu17Shinji Kasai18Kyoko Sawabe19Haruhiko Isawa20Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanInstitute of Tropical Diseases, Airlangga University, Surabaya, IndonesiaFaculty of Fisheries and Marine, Universitas Airlangga, Surabaya, IndonesiaInstitute of Tropical Diseases, Airlangga University, Surabaya, IndonesiaInstitute of Tropical Diseases, Airlangga University, Surabaya, IndonesiaDivision of Clinical Virology, Center for Infectious Diseases, Kobe University, Kobe, JapanFaculty of Medicine, Ege University, Bornova, Izmir, TurkiyeLaboratory of Molecular Immunology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, JapanDepartment of Medical Entomology and Zoology, National Institute of Hygiene and Epidemiology, Hanoi, VietnamDepartment of Medical Entomology and Zoology, National Institute of Hygiene and Epidemiology, Hanoi, VietnamDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanDepartment of Medical Entomology, National Institute of Infectious Diseases, Shinjuku, JapanJapanese encephalitis virus (JEV) genotype IV (GIV) is one of the least common and most neglected genotypes worldwide, having been identified only on a few Indonesian islands until it was recently found to be the cause of outbreaks that occurred in several Australian states in early 2022. Given the limited availability of information, the vector range for JEV GIV remains unknown; thus, understanding this range could prove invaluable for future prevention efforts in new areas. Herein, we experimentally exposed four mosquito colonies originated from various countries with no previous reports of GIV to JEV GIV strain 19CxBa-83-Cv, which was isolated from Culex vishnui Theobald collected in Bali in 2019. At 7 and 14 days post-JEV GIV exposure through a membrane feeding method, mosquito bodies, head–wings–legs, and saliva were harvested for infection, dissemination, and transmission efficiency analyses. The results showed robust transmission efficiencies of the virus by Culex tritaeniorhynchus Giles (∼74%) and Aedes albopictus Skuse (∼52%) from Japan, followed by Culex quinquefasciatus Say from Vietnam (∼35%) and Culex pipiens form molestus from Turkey (∼18%). Although significant differences were observed, we found that the four mosquito species could transmit JEV GIV. The efficiency of biological transmission of this restricted genotype by mosquitoes from various origins suggests that these mosquito species could support localized transmission if the genotype were introduced to their respective areas. This study emphasizes the importance of remaining vigilant and continuing arbovirus surveillance in all locations.https://www.tandfonline.com/doi/10.1080/22221751.2024.2438661Japanese encephalitis virusgenotype IVAe. albopictusCx. pipiensCx. tritaeniorhynchusCx. quinquefasciatus |
| spellingShingle | Astri Nur Faizah Daisuke Kobayashi Faustus Akankperiwen Azerigyik Ryo Matsumura Izumi Kai Yoshihide Maekawa Yukiko Higa Kentaro Itokawa Toshinori Sasaki Kris Cahyo Mulyatno Sri Subekti Maria Inge Lusida Etik Ainun Rohmah Yasuko Mori Yusuf Ozbel Chizu Sanjoba Tran Vu Phong Tran Cong Tu Shinji Kasai Kyoko Sawabe Haruhiko Isawa Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV Emerging Microbes and Infections Japanese encephalitis virus genotype IV Ae. albopictus Cx. pipiens Cx. tritaeniorhynchus Cx. quinquefasciatus |
| title | Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV |
| title_full | Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV |
| title_fullStr | Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV |
| title_full_unstemmed | Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV |
| title_short | Mosquito populations originating from nonendemic areas have the potential to transmit recently emerging Japanese encephalitis virus genotype IV |
| title_sort | mosquito populations originating from nonendemic areas have the potential to transmit recently emerging japanese encephalitis virus genotype iv |
| topic | Japanese encephalitis virus genotype IV Ae. albopictus Cx. pipiens Cx. tritaeniorhynchus Cx. quinquefasciatus |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2024.2438661 |
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