Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects
Objectives: Advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) have recently been developed and used in periodontal therapy. Few studies have contrasted the antibacterial effectiveness of these autologous materials derived from individuals with healthy gums, gingiviti...
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Elsevier
2023-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426823000234 |
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| author | Thuy Anh Vu Pham Thao Thi Phuong Tran |
| author_facet | Thuy Anh Vu Pham Thao Thi Phuong Tran |
| author_sort | Thuy Anh Vu Pham |
| collection | DOAJ |
| description | Objectives: Advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) have recently been developed and used in periodontal therapy. Few studies have contrasted the antibacterial effectiveness of these autologous materials derived from individuals with healthy gums, gingivitis, and periodontitis. This study aimed to compare the antimicrobial effects of these PRF materials against the periodontal pathogenic bacterium Aggregatibacter actinomycetemcomitans (Aa) in patients with different periodontal conditions. Methods: Blood samples were collected from periodontally healthy individuals, patients with gingivitis, or patients with periodontitis to prepare A-PRF+ and i-PRF. The antibacterial capacity of these materials was evaluated through antibiofilm formation, biofilm susceptibility, and the time-kill assay over a 48-h period. Results: A-PRF+ and i-PRF from each patient groups interfered with Aa's ability to form biofilm on the test tube surface, and the effect of i-PRF was significantly different among the patient groups. In contrast, these plasma preparation had a weak impact on mature biofilm. For products from the gingivitis and periodontitis groups, these effects were significantly stronger for i-PRF than A-PRF+ (p = 0.012 and p = 0.004, respectively). All plasma preparations inhibited Aa growth in the first 12 h after application, and i-PRF exhibited a significantly greater antimicrobial effect than A-PRF + at each time point. Conclusion: A-PRF+ and i-PRF in all three patient groups could inhibit the growth of Aa in vitro, and i-PRF from patients with periodontitis exhibited a more significant effect than PRF from the other groups. |
| format | Article |
| id | doaj-art-359e8bb0d3f148a1b8e7eb387cdc4310 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-359e8bb0d3f148a1b8e7eb387cdc43102024-11-23T06:30:00ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682023-03-01132332336Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjectsThuy Anh Vu Pham0Thao Thi Phuong Tran1Division of Odonto-Stomatology, School of Medicine, Ho Chi Minh City, Viet Nam; Vietnam National University, Ho Chi Minh City, Viet Nam; Corresponding author. Division of Odonto-Stomatology, School of Medicine, Vietnam National University, Ho Chi Minh City, Viet Nam.Faculty of Odonto-Stomatology, Hong Bang International University, Viet NamObjectives: Advanced platelet-rich fibrin (A-PRF+) and injectable platelet-rich fibrin (i-PRF) have recently been developed and used in periodontal therapy. Few studies have contrasted the antibacterial effectiveness of these autologous materials derived from individuals with healthy gums, gingivitis, and periodontitis. This study aimed to compare the antimicrobial effects of these PRF materials against the periodontal pathogenic bacterium Aggregatibacter actinomycetemcomitans (Aa) in patients with different periodontal conditions. Methods: Blood samples were collected from periodontally healthy individuals, patients with gingivitis, or patients with periodontitis to prepare A-PRF+ and i-PRF. The antibacterial capacity of these materials was evaluated through antibiofilm formation, biofilm susceptibility, and the time-kill assay over a 48-h period. Results: A-PRF+ and i-PRF from each patient groups interfered with Aa's ability to form biofilm on the test tube surface, and the effect of i-PRF was significantly different among the patient groups. In contrast, these plasma preparation had a weak impact on mature biofilm. For products from the gingivitis and periodontitis groups, these effects were significantly stronger for i-PRF than A-PRF+ (p = 0.012 and p = 0.004, respectively). All plasma preparations inhibited Aa growth in the first 12 h after application, and i-PRF exhibited a significantly greater antimicrobial effect than A-PRF + at each time point. Conclusion: A-PRF+ and i-PRF in all three patient groups could inhibit the growth of Aa in vitro, and i-PRF from patients with periodontitis exhibited a more significant effect than PRF from the other groups.http://www.sciencedirect.com/science/article/pii/S2212426823000234Antimicrobial effectAggregatibacter actinomycetemcomitansAdvanced platelet rich fibrinInjectable platelet rich fibrinBacterial biofilmTime-kill assay |
| spellingShingle | Thuy Anh Vu Pham Thao Thi Phuong Tran Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects Journal of Oral Biology and Craniofacial Research Antimicrobial effect Aggregatibacter actinomycetemcomitans Advanced platelet rich fibrin Injectable platelet rich fibrin Bacterial biofilm Time-kill assay |
| title | Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| title_full | Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| title_fullStr | Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| title_full_unstemmed | Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| title_short | Antimicrobial effect against Aggregatibacter actinomycetemcomitans of advanced and injectable platelet-rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| title_sort | antimicrobial effect against aggregatibacter actinomycetemcomitans of advanced and injectable platelet rich fibrin from patients with periodontal diseases versus periodontally healthy subjects |
| topic | Antimicrobial effect Aggregatibacter actinomycetemcomitans Advanced platelet rich fibrin Injectable platelet rich fibrin Bacterial biofilm Time-kill assay |
| url | http://www.sciencedirect.com/science/article/pii/S2212426823000234 |
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