Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics
Oleuropein is a phenolic compound commonly found in cosmetic ingredients including olive leaves and jasmine flowers with various skin-beneficial effects. Here, we evaluated oleuropein’s anti-inflammatory and antioxidant activities in human skin cells. In a cell-based inflammasome model with human mo...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1496078/full |
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| author | Huifang Li Ni Deng Jiayi Yang Yang Zhao Xiaoxuan Jin Ang Cai Navindra P. Seeram Hang Ma Dongli Li Huilan Yang Chang Liu Chang Liu |
| author_facet | Huifang Li Ni Deng Jiayi Yang Yang Zhao Xiaoxuan Jin Ang Cai Navindra P. Seeram Hang Ma Dongli Li Huilan Yang Chang Liu Chang Liu |
| author_sort | Huifang Li |
| collection | DOAJ |
| description | Oleuropein is a phenolic compound commonly found in cosmetic ingredients including olive leaves and jasmine flowers with various skin-beneficial effects. Here, we evaluated oleuropein’s anti-inflammatory and antioxidant activities in human skin cells. In a cell-based inflammasome model with human monocytes (THP-1 cells), oleuropein (12–200 µM) reduced proinflammatory cytokine interleukin (IL)-6 by 38.8%–45.5%, respectively. Oleuropein (50 and 100 µM) also alleviated oxidative stress in keratinocytes (HaCaT cells) by reducing H2O2-induced cell death by 6.4% and 9.2%, respectively. Additionally, biological evaluations revealed that oleuropein’s antioxidant effects were attributed to its mitigation of reactive oxygen species in HaCaT cells. Furthermore, a multiplexed gene assay identified IL-1β and thioredoxin-interacting proteins as potential molecular targets involved in oleuropein’s protective effects in HaCaT cells. This was supported by findings from several cellular assays showing that oleuropein reduced the level of IL-1β and inhibited the activity of caspase-1/IL-1 converting enzyme, as well as ameliorated pyroptosis in HaCaT cells. Moreover, a bottom-up proteomics study was conducted to explore potential molecular targets and signaling pathways involved in oleuropein’s antioxidant activities. Taken together, findings from this study expand the understanding of oleuropein’s skin protective effects against oxidative and inflammatory stresses, which support that oleuropein is a promising natural cosmeceutical for skincare applications. |
| format | Article |
| id | doaj-art-359065ab5b3e4412958aa911ab9847db |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-359065ab5b3e4412958aa911ab9847db2025-01-08T06:12:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.14960781496078Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomicsHuifang Li0Ni Deng1Jiayi Yang2Yang Zhao3Xiaoxuan Jin4Ang Cai5Navindra P. Seeram6Hang Ma7Dongli Li8Huilan Yang9Chang Liu10Chang Liu11Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesProteomics Facility, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesBioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesOutpatient Department, Southern Theater Command General Hospital, Guangzhou, ChinaOutpatient Department, Southern Theater Command General Hospital, Guangzhou, ChinaProteomics Facility, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesBioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesBioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesSchool of Pharmacy and Food Engineering, Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, ChinaOutpatient Department, Southern Theater Command General Hospital, Guangzhou, ChinaBioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesProteomics Facility, College of Pharmacy, University of Rhode Island, Kingston, RI, United StatesOleuropein is a phenolic compound commonly found in cosmetic ingredients including olive leaves and jasmine flowers with various skin-beneficial effects. Here, we evaluated oleuropein’s anti-inflammatory and antioxidant activities in human skin cells. In a cell-based inflammasome model with human monocytes (THP-1 cells), oleuropein (12–200 µM) reduced proinflammatory cytokine interleukin (IL)-6 by 38.8%–45.5%, respectively. Oleuropein (50 and 100 µM) also alleviated oxidative stress in keratinocytes (HaCaT cells) by reducing H2O2-induced cell death by 6.4% and 9.2%, respectively. Additionally, biological evaluations revealed that oleuropein’s antioxidant effects were attributed to its mitigation of reactive oxygen species in HaCaT cells. Furthermore, a multiplexed gene assay identified IL-1β and thioredoxin-interacting proteins as potential molecular targets involved in oleuropein’s protective effects in HaCaT cells. This was supported by findings from several cellular assays showing that oleuropein reduced the level of IL-1β and inhibited the activity of caspase-1/IL-1 converting enzyme, as well as ameliorated pyroptosis in HaCaT cells. Moreover, a bottom-up proteomics study was conducted to explore potential molecular targets and signaling pathways involved in oleuropein’s antioxidant activities. Taken together, findings from this study expand the understanding of oleuropein’s skin protective effects against oxidative and inflammatory stresses, which support that oleuropein is a promising natural cosmeceutical for skincare applications.https://www.frontiersin.org/articles/10.3389/fphar.2024.1496078/fulloleuropeinskinantioxidantinflammasomecaspaseproteomics |
| spellingShingle | Huifang Li Ni Deng Jiayi Yang Yang Zhao Xiaoxuan Jin Ang Cai Navindra P. Seeram Hang Ma Dongli Li Huilan Yang Chang Liu Chang Liu Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics Frontiers in Pharmacology oleuropein skin antioxidant inflammasome caspase proteomics |
| title | Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics |
| title_full | Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics |
| title_fullStr | Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics |
| title_full_unstemmed | Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics |
| title_short | Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics |
| title_sort | anti inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom up proteomics |
| topic | oleuropein skin antioxidant inflammasome caspase proteomics |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1496078/full |
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