Marker-assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L
Four varieties of Brassica napus, Xiangyou 15, Huyou 15, You 88 and Huyou 17, were crossed with a temporary maintainer (TAM) named HYZ1-TAM respectively and produced four F<sub>2</sub> populations. In these populations, molecular markers associated with Bnms3 and Bnrf were used to select...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.008 |
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| Summary: | Four varieties of Brassica napus, Xiangyou 15, Huyou 15, You 88 and Huyou 17, were crossed with a temporary maintainer (TAM) named HYZ1-TAM respectively and produced four F<sub>2</sub> populations. In these populations, molecular markers associated with Bnms3 and Bnrf were used to select new temporary maintainer plants which were identified by test-cross. Sixty-two plants with TAM marker-genotype were selected by marker-assisted selection (MAS) from three hundred and fifty-five plants of four F<sub>2</sub> populations. Fifty-seven plants (with the accuracy of 91.94%) were TAM genotype proved by test-cross. Compared with traditional method, it is very efficient to select TAM by MAS. In the Bnms4 locus, all the four varieties have a same genotype which is ms4ms4. |
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| ISSN: | 1008-9209 2097-5155 |