Heat application in live cell imaging
Thermal heating of biological samples allows to reversibly manipulate cellular processes with high temporal and spatial resolution. Manifold heating techniques in combination with live‐cell imaging were developed, commonly tailored to customized applications. They include Peltier elements and microf...
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| Format: | Article |
| Language: | English |
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Wiley
2024-12-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13912 |
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| _version_ | 1846143850588930048 |
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| author | Linda Sistemich Simon Ebbinghaus |
| author_facet | Linda Sistemich Simon Ebbinghaus |
| author_sort | Linda Sistemich |
| collection | DOAJ |
| description | Thermal heating of biological samples allows to reversibly manipulate cellular processes with high temporal and spatial resolution. Manifold heating techniques in combination with live‐cell imaging were developed, commonly tailored to customized applications. They include Peltier elements and microfluidics for homogenous sample heating as well as infrared lasers and radiation absorption by nanostructures for spot heating. A prerequisite of all techniques is that the induced temperature changes are measured precisely which can be the main challenge considering subcellular structures or multicellular organisms as target regions. This article discusses heating and temperature sensing techniques for live‐cell imaging, leading to future applications in cell biology. |
| format | Article |
| id | doaj-art-350735dc18764ad192e1436815f0f33d |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-350735dc18764ad192e1436815f0f33d2024-12-02T09:49:10ZengWileyFEBS Open Bio2211-54632024-12-0114121940195410.1002/2211-5463.13912Heat application in live cell imagingLinda Sistemich0Simon Ebbinghaus1Chair of Biophysical Chemistry Ruhr‐University Bochum GermanyChair of Biophysical Chemistry Ruhr‐University Bochum GermanyThermal heating of biological samples allows to reversibly manipulate cellular processes with high temporal and spatial resolution. Manifold heating techniques in combination with live‐cell imaging were developed, commonly tailored to customized applications. They include Peltier elements and microfluidics for homogenous sample heating as well as infrared lasers and radiation absorption by nanostructures for spot heating. A prerequisite of all techniques is that the induced temperature changes are measured precisely which can be the main challenge considering subcellular structures or multicellular organisms as target regions. This article discusses heating and temperature sensing techniques for live‐cell imaging, leading to future applications in cell biology.https://doi.org/10.1002/2211-5463.13912fluorescence microscopylive‐cell imagingmicroheatingthermometry |
| spellingShingle | Linda Sistemich Simon Ebbinghaus Heat application in live cell imaging FEBS Open Bio fluorescence microscopy live‐cell imaging microheating thermometry |
| title | Heat application in live cell imaging |
| title_full | Heat application in live cell imaging |
| title_fullStr | Heat application in live cell imaging |
| title_full_unstemmed | Heat application in live cell imaging |
| title_short | Heat application in live cell imaging |
| title_sort | heat application in live cell imaging |
| topic | fluorescence microscopy live‐cell imaging microheating thermometry |
| url | https://doi.org/10.1002/2211-5463.13912 |
| work_keys_str_mv | AT lindasistemich heatapplicationinlivecellimaging AT simonebbinghaus heatapplicationinlivecellimaging |