Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins
Senecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Veterinary Quarterly |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/01652176.2024.2449082 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841554926504771584 |
|---|---|
| author | Parin Watcharavongtip Patumporn Jermsutjarit Angkana Tantituvanont Dachrit Nilubol |
| author_facet | Parin Watcharavongtip Patumporn Jermsutjarit Angkana Tantituvanont Dachrit Nilubol |
| author_sort | Parin Watcharavongtip |
| collection | DOAJ |
| description | Senecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non-structural viral protein has been developed, capable of distinguishing infected from vaccinated animals (DIVA). Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. The baculovirus-expressed SVA 3AB DIVA ELISA demonstrated a sensitivity of 96.67% and specificity of 96.67%. In contrast, the E. coli-expressed SVA 3AB DIVA ELISA achieved 100% sensitivity and 93.33% specificity. Both ELISAs strongly correlated with the reference method and showed no cross-reactivity with other pig pathogens. The E. coli system also provided a higher yield of expressed protein than the baculovirus system. These findings indicate that SVA DIVA ELISAs are effective alternatives for detecting SVA antibodies. They can be valuable tools for sero-surveillance and for evaluating immunity status tests to support and approve vaccination programs for pig herds in the future. |
| format | Article |
| id | doaj-art-34c9fe385c2c4d9aa277ed0c42d73bc8 |
| institution | Kabale University |
| issn | 0165-2176 1875-5941 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Veterinary Quarterly |
| spelling | doaj-art-34c9fe385c2c4d9aa277ed0c42d73bc82025-01-08T08:18:58ZengTaylor & Francis GroupVeterinary Quarterly0165-21761875-59412025-12-0145111110.1080/01652176.2024.2449082Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteinsParin Watcharavongtip0Patumporn Jermsutjarit1Angkana Tantituvanont2Dachrit Nilubol3Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandSwine Viral Evolution and Vaccine Development Research Unit, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandSenecavirus A (SVA) is the causative agent associated with porcine idiopathic vesicular disease (PIVD), a condition indistinguishable from other foreign vesicular diseases affecting pigs. This complicates differential diagnosis and impacts the global swine industry. A diagnostic ELISA based on a non-structural viral protein has been developed, capable of distinguishing infected from vaccinated animals (DIVA). Different expression systems (eukaryotic and prokaryotic) were used to express recombinant proteins. The baculovirus-expressed SVA 3AB DIVA ELISA demonstrated a sensitivity of 96.67% and specificity of 96.67%. In contrast, the E. coli-expressed SVA 3AB DIVA ELISA achieved 100% sensitivity and 93.33% specificity. Both ELISAs strongly correlated with the reference method and showed no cross-reactivity with other pig pathogens. The E. coli system also provided a higher yield of expressed protein than the baculovirus system. These findings indicate that SVA DIVA ELISAs are effective alternatives for detecting SVA antibodies. They can be valuable tools for sero-surveillance and for evaluating immunity status tests to support and approve vaccination programs for pig herds in the future.https://www.tandfonline.com/doi/10.1080/01652176.2024.2449082Senecavirus Aantibodyindirect ELISADIVApig |
| spellingShingle | Parin Watcharavongtip Patumporn Jermsutjarit Angkana Tantituvanont Dachrit Nilubol Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins Veterinary Quarterly Senecavirus A antibody indirect ELISA DIVA pig |
| title | Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins |
| title_full | Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins |
| title_fullStr | Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins |
| title_full_unstemmed | Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins |
| title_short | Development of a differentiating of infected from vaccinated animal (DIVA) ELISA to detect antibodies against Senecavirus A in pigs using two expression systems of non-structural proteins |
| title_sort | development of a differentiating of infected from vaccinated animal diva elisa to detect antibodies against senecavirus a in pigs using two expression systems of non structural proteins |
| topic | Senecavirus A antibody indirect ELISA DIVA pig |
| url | https://www.tandfonline.com/doi/10.1080/01652176.2024.2449082 |
| work_keys_str_mv | AT parinwatcharavongtip developmentofadifferentiatingofinfectedfromvaccinatedanimaldivaelisatodetectantibodiesagainstsenecavirusainpigsusingtwoexpressionsystemsofnonstructuralproteins AT patumpornjermsutjarit developmentofadifferentiatingofinfectedfromvaccinatedanimaldivaelisatodetectantibodiesagainstsenecavirusainpigsusingtwoexpressionsystemsofnonstructuralproteins AT angkanatantituvanont developmentofadifferentiatingofinfectedfromvaccinatedanimaldivaelisatodetectantibodiesagainstsenecavirusainpigsusingtwoexpressionsystemsofnonstructuralproteins AT dachritnilubol developmentofadifferentiatingofinfectedfromvaccinatedanimaldivaelisatodetectantibodiesagainstsenecavirusainpigsusingtwoexpressionsystemsofnonstructuralproteins |