EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE
The results of the effect of pulse-periodic X-ray exposure (PPXE) generated by Sinus-150 accelerator on tumor and normal cells of mice have been presented. Chromosomal aberrations (metaphase analysis) of bone marrow cells were studied. Using the cultured Lewis lung carcinoma cell lines, the level of...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Russian Academy of Sciences, Tomsk National Research Medical Center
2016-02-01
|
| Series: | Сибирский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.siboncoj.ru/jour/article/view/271 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849253238186967040 |
|---|---|
| author | М. А. Buldakov N. V. Litvyakov I. А. Klimov О. P. Kutenkov А. А. Melnikov М. А. Bolshakov V. V. Rostov N. V. Cherdyntseva |
| author_facet | М. А. Buldakov N. V. Litvyakov I. А. Klimov О. P. Kutenkov А. А. Melnikov М. А. Bolshakov V. V. Rostov N. V. Cherdyntseva |
| author_sort | М. А. Buldakov |
| collection | DOAJ |
| description | The results of the effect of pulse-periodic X-ray exposure (PPXE) generated by Sinus-150 accelerator on tumor and normal cells of mice have been presented. Chromosomal aberrations (metaphase analysis) of bone marrow cells were studied. Using the cultured Lewis lung carcinoma cell lines, the level of apoptotic cells and cells with high concentration of active oxygen forms (AOF) was assessed by the flow cytofluorometry analysis (FACSCantoII, BD). In vivo X-ray radiation in the pulse-periodic mode lead to the moderate increase in chromosomal aberrations of bone marrow cells of mice. PPXE induced apoptotic death in Lewis lung carcinoma cells in vitro due to AOF production. Dose-effect relation was not observed at the irradiation doses 0.2–1.0 Gy. The data obtained show that further studies are necessary to justify the use of PPXE in tumor therapy. |
| format | Article |
| id | doaj-art-34a301eedb8943a4bcb36f3d3f52ffc9 |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2016-02-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-34a301eedb8943a4bcb36f3d3f52ffc92025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682016-02-01053438271EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICEМ. А. Buldakov0N. V. Litvyakov1I. А. Klimov2О. P. KutenkovА. А. Melnikov3М. А. Bolshakov4V. V. Rostov5N. V. Cherdyntseva6Tomsk Cancer Research Institute Tomsk State University, TomskTomsk Cancer Research Institute Tomsk State University, TomskTomsk Cancer Research InstituteTomsk Cancer Research InstituteInstitute of High-Current Electronics, Siberian Division, Russian Academy of Sciences, Tomsk Tomsk State University, TomskInstitute of High-Current Electronics, Siberian Division, Russian Academy of Sciences, Tomsk Tomsk State University, TomskTomsk Cancer Research Institute Tomsk State University, TomskThe results of the effect of pulse-periodic X-ray exposure (PPXE) generated by Sinus-150 accelerator on tumor and normal cells of mice have been presented. Chromosomal aberrations (metaphase analysis) of bone marrow cells were studied. Using the cultured Lewis lung carcinoma cell lines, the level of apoptotic cells and cells with high concentration of active oxygen forms (AOF) was assessed by the flow cytofluorometry analysis (FACSCantoII, BD). In vivo X-ray radiation in the pulse-periodic mode lead to the moderate increase in chromosomal aberrations of bone marrow cells of mice. PPXE induced apoptotic death in Lewis lung carcinoma cells in vitro due to AOF production. Dose-effect relation was not observed at the irradiation doses 0.2–1.0 Gy. The data obtained show that further studies are necessary to justify the use of PPXE in tumor therapy.https://www.siboncoj.ru/jour/article/view/271pulse-periodic x-ray exposurechromosomal aberrationsapoptosisbone marrowexperimental tumors |
| spellingShingle | М. А. Buldakov N. V. Litvyakov I. А. Klimov О. P. Kutenkov А. А. Melnikov М. А. Bolshakov V. V. Rostov N. V. Cherdyntseva EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE Сибирский онкологический журнал pulse-periodic x-ray exposure chromosomal aberrations apoptosis bone marrow experimental tumors |
| title | EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE |
| title_full | EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE |
| title_fullStr | EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE |
| title_full_unstemmed | EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE |
| title_short | EFFECT OF PULSE PERIODIC X-RAYS ON TUMOR AND BONE MARROW CELLS OF MICE |
| title_sort | effect of pulse periodic x rays on tumor and bone marrow cells of mice |
| topic | pulse-periodic x-ray exposure chromosomal aberrations apoptosis bone marrow experimental tumors |
| url | https://www.siboncoj.ru/jour/article/view/271 |
| work_keys_str_mv | AT mabuldakov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT nvlitvyakov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT iaklimov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT opkutenkov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT aamelnikov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT mabolshakov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT vvrostov effectofpulseperiodicxraysontumorandbonemarrowcellsofmice AT nvcherdyntseva effectofpulseperiodicxraysontumorandbonemarrowcellsofmice |