Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides

<i>Vitis vinifera</i> is highly susceptible to grapevine trunk diseases, with <i>Neofusicoccum parvum</i> recognized as a highly destructive pathogen. This study investigates the biocontrol potential of five <i>Trichoderma</i> species (<i>T. harzianum</i&...

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Main Authors: Aranzazu Gomez-Garay, Sergio Astudillo Calderón, Mª Luisa Tello Mariscal, Beatriz Pintos López
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/12/2766
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author Aranzazu Gomez-Garay
Sergio Astudillo Calderón
Mª Luisa Tello Mariscal
Beatriz Pintos López
author_facet Aranzazu Gomez-Garay
Sergio Astudillo Calderón
Mª Luisa Tello Mariscal
Beatriz Pintos López
author_sort Aranzazu Gomez-Garay
collection DOAJ
description <i>Vitis vinifera</i> is highly susceptible to grapevine trunk diseases, with <i>Neofusicoccum parvum</i> recognized as a highly destructive pathogen. This study investigates the biocontrol potential of five <i>Trichoderma</i> species (<i>T. harzianum</i>, <i>T. viride</i>, <i>T. asperellum</i>, and <i>T. virens</i>) against <i>N. parvum</i>, evaluating multiple biocontrol mechanisms (mycoparasitism, competition for nutrients, production of volatile organic compounds (VOCs), and antibiosis) as well as their compatibility with the fungicides copper oxychloride and sulfur. Results demonstrated that (1) <i>Trichoderma harzianum</i> effectively suppressed <i>N. parvum</i> through VOC production, mycoparasitism, and nutrient competition, significantly reducing pathogen growth <i>in planta</i> while showing compatibility with both fungicides, highlighting its suitability for integrated disease management; (2) <i>Trichoderma viride</i> showed high inhibition of <i>N. parvum</i> in vitro, but its phytotoxicity <i>in planta</i> limits its field application. These findings support <i>T. harzianum</i> as a promising agent within integrated disease management strategies, offering a sustainable alternative to reduce chemical fungicide reliance in controlling grapevine trunk pathogens.
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spelling doaj-art-3478f13e7c1e40be99118e6e7792e7a32024-12-27T14:03:53ZengMDPI AGAgronomy2073-43952024-11-011412276610.3390/agronomy14122766Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical FungicidesAranzazu Gomez-Garay0Sergio Astudillo Calderón1Mª Luisa Tello Mariscal2Beatriz Pintos López3Research Group FiVe-A, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, SpainResearch Group FiVe-A, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, SpainInstituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria—Spanish National Research Council, Ministry of Science, Innovation and Universities, 28040 Madrid, SpainResearch Group FiVe-A, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain<i>Vitis vinifera</i> is highly susceptible to grapevine trunk diseases, with <i>Neofusicoccum parvum</i> recognized as a highly destructive pathogen. This study investigates the biocontrol potential of five <i>Trichoderma</i> species (<i>T. harzianum</i>, <i>T. viride</i>, <i>T. asperellum</i>, and <i>T. virens</i>) against <i>N. parvum</i>, evaluating multiple biocontrol mechanisms (mycoparasitism, competition for nutrients, production of volatile organic compounds (VOCs), and antibiosis) as well as their compatibility with the fungicides copper oxychloride and sulfur. Results demonstrated that (1) <i>Trichoderma harzianum</i> effectively suppressed <i>N. parvum</i> through VOC production, mycoparasitism, and nutrient competition, significantly reducing pathogen growth <i>in planta</i> while showing compatibility with both fungicides, highlighting its suitability for integrated disease management; (2) <i>Trichoderma viride</i> showed high inhibition of <i>N. parvum</i> in vitro, but its phytotoxicity <i>in planta</i> limits its field application. These findings support <i>T. harzianum</i> as a promising agent within integrated disease management strategies, offering a sustainable alternative to reduce chemical fungicide reliance in controlling grapevine trunk pathogens.https://www.mdpi.com/2073-4395/14/12/2766biocontrolgrapevine trunk disease<i>N. parvum</i><i>Trichoderma</i> spp.integrated disease management
spellingShingle Aranzazu Gomez-Garay
Sergio Astudillo Calderón
Mª Luisa Tello Mariscal
Beatriz Pintos López
Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
Agronomy
biocontrol
grapevine trunk disease
<i>N. parvum</i>
<i>Trichoderma</i> spp.
integrated disease management
title Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
title_full Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
title_fullStr Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
title_full_unstemmed Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
title_short Effective Control of <i>Neofusicoccum parvum</i> in Grapevines: Combining <i>Trichoderma</i> spp. with Chemical Fungicides
title_sort effective control of i neofusicoccum parvum i in grapevines combining i trichoderma i spp with chemical fungicides
topic biocontrol
grapevine trunk disease
<i>N. parvum</i>
<i>Trichoderma</i> spp.
integrated disease management
url https://www.mdpi.com/2073-4395/14/12/2766
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