The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy
The existence of intra-amniotic and placental microbiomes during pregnancy has been the source of considerable debate, with research yielding conflicting evidence. This study evaluated investigations into the putative presence of these microbiomes in healthy pregnancies by identifying design and dat...
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MDPI AG
2024-10-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/13/11/888 |
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| author | Kumar Uddipto Julie A. Quinlivan George L. Mendz |
| author_facet | Kumar Uddipto Julie A. Quinlivan George L. Mendz |
| author_sort | Kumar Uddipto |
| collection | DOAJ |
| description | The existence of intra-amniotic and placental microbiomes during pregnancy has been the source of considerable debate, with research yielding conflicting evidence. This study evaluated investigations into the putative presence of these microbiomes in healthy pregnancies by identifying design and data interpretation issues, particularly concerning contamination in samples with low-density bacterial DNA. Positive findings from diverse populations suggest a consistent presence of microbiota in the intra-amniotic space. Negative conclusions regarding the existence of these prenatal microbiomes emphasise the impact of contamination in the analysis of samples with low-density bacterial DNA. This study concluded that there is no definitive evidence to refute the existence of intra-amniotic microbiomes in healthy pregnancies. Also, it provides suggestions for controlling potential contamination factors in future research on intra-amniotic and placental microbial populations. |
| format | Article |
| id | doaj-art-343a9c497b564b9c80cf2d8bc51038f0 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-343a9c497b564b9c80cf2d8bc51038f02024-11-26T17:52:20ZengMDPI AGBiology2079-77372024-10-01131188810.3390/biology13110888The Existence of an Intra-Amniotic Microbiome: Assessing a ControversyKumar Uddipto0Julie A. Quinlivan1George L. Mendz2School of Medicine, Sydney Program, The University of Notre Dame Australia, 160 Oxford St., Darlinghurst, NSW 2010, AustraliaInstitute for Health Research, The University of Notre Dame Australia, 32 Mouat St., Fremantle, WA 6160, AustraliaSchool of Medicine, Sydney Program, The University of Notre Dame Australia, 160 Oxford St., Darlinghurst, NSW 2010, AustraliaThe existence of intra-amniotic and placental microbiomes during pregnancy has been the source of considerable debate, with research yielding conflicting evidence. This study evaluated investigations into the putative presence of these microbiomes in healthy pregnancies by identifying design and data interpretation issues, particularly concerning contamination in samples with low-density bacterial DNA. Positive findings from diverse populations suggest a consistent presence of microbiota in the intra-amniotic space. Negative conclusions regarding the existence of these prenatal microbiomes emphasise the impact of contamination in the analysis of samples with low-density bacterial DNA. This study concluded that there is no definitive evidence to refute the existence of intra-amniotic microbiomes in healthy pregnancies. Also, it provides suggestions for controlling potential contamination factors in future research on intra-amniotic and placental microbial populations.https://www.mdpi.com/2079-7737/13/11/888bacterial taxaamplicon sequencingintra-amniotic microbiomeenvironmental contamination |
| spellingShingle | Kumar Uddipto Julie A. Quinlivan George L. Mendz The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy Biology bacterial taxa amplicon sequencing intra-amniotic microbiome environmental contamination |
| title | The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy |
| title_full | The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy |
| title_fullStr | The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy |
| title_full_unstemmed | The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy |
| title_short | The Existence of an Intra-Amniotic Microbiome: Assessing a Controversy |
| title_sort | existence of an intra amniotic microbiome assessing a controversy |
| topic | bacterial taxa amplicon sequencing intra-amniotic microbiome environmental contamination |
| url | https://www.mdpi.com/2079-7737/13/11/888 |
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