Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment
We developed a tailored 3D Agarose-well system integrated with reconstructed human skin equivalents to enhance skin penetration assessments. This system addresses common limitations in traditional trans-well reconstructions, such as dermal layer contraction and limited lateral diffusion, by entangli...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/10/11/691 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846153425588322304 |
|---|---|
| author | Chaewon Woo Jina Byun Sung Gyu Shin Heeseon Yoo Sungwoo Cho Donghun Lee Taezoon Park Jae Hyun Jeong |
| author_facet | Chaewon Woo Jina Byun Sung Gyu Shin Heeseon Yoo Sungwoo Cho Donghun Lee Taezoon Park Jae Hyun Jeong |
| author_sort | Chaewon Woo |
| collection | DOAJ |
| description | We developed a tailored 3D Agarose-well system integrated with reconstructed human skin equivalents to enhance skin penetration assessments. This system addresses common limitations in traditional trans-well reconstructions, such as dermal layer contraction and limited lateral diffusion, by entangling collagen fibrils within the Agarose-well. We evaluated the penetration behavior of three peptides, with and without skin-penetrating peptide (SPP) sequences, alongside adenosine, a known anti-wrinkle agent. Despite a SPP having a molecular weight approximately four times greater than that of adenosine, its kinetic constant was similar, with values of about 39 and 34, respectively. Moreover, this living skin equivalent system not only allowed for the evaluation of adenosine penetration, but also demonstrated its biological effects, with adenosine significantly enhancing procollagen synthesis by approximately 23% compared to the control. Overall, this novel strategy holds the potential for tailoring 3D Agarose-wells and advancing high-performance gel development, making it a promising approach for applications in tissue engineering, medical science, regenerative medicine, and cosmetics. |
| format | Article |
| id | doaj-art-342114ff5afb443983dbeea83aa0197f |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-342114ff5afb443983dbeea83aa0197f2024-11-26T18:05:23ZengMDPI AGGels2310-28612024-10-01101169110.3390/gels10110691Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration AssessmentChaewon Woo0Jina Byun1Sung Gyu Shin2Heeseon Yoo3Sungwoo Cho4Donghun Lee5Taezoon Park6Jae Hyun Jeong7Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Mechanical Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Industrial & Information Systems Engineering, Soongsil University, Seoul 06978, Republic of KoreaDepartment of Chemical Engineering, Soongsil University, Seoul 06978, Republic of KoreaWe developed a tailored 3D Agarose-well system integrated with reconstructed human skin equivalents to enhance skin penetration assessments. This system addresses common limitations in traditional trans-well reconstructions, such as dermal layer contraction and limited lateral diffusion, by entangling collagen fibrils within the Agarose-well. We evaluated the penetration behavior of three peptides, with and without skin-penetrating peptide (SPP) sequences, alongside adenosine, a known anti-wrinkle agent. Despite a SPP having a molecular weight approximately four times greater than that of adenosine, its kinetic constant was similar, with values of about 39 and 34, respectively. Moreover, this living skin equivalent system not only allowed for the evaluation of adenosine penetration, but also demonstrated its biological effects, with adenosine significantly enhancing procollagen synthesis by approximately 23% compared to the control. Overall, this novel strategy holds the potential for tailoring 3D Agarose-wells and advancing high-performance gel development, making it a promising approach for applications in tissue engineering, medical science, regenerative medicine, and cosmetics.https://www.mdpi.com/2310-2861/10/11/6913D agarose-wellskin penetrationhuman skin equivalentshigh-performance gels |
| spellingShingle | Chaewon Woo Jina Byun Sung Gyu Shin Heeseon Yoo Sungwoo Cho Donghun Lee Taezoon Park Jae Hyun Jeong Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment Gels 3D agarose-well skin penetration human skin equivalents high-performance gels |
| title | Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment |
| title_full | Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment |
| title_fullStr | Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment |
| title_full_unstemmed | Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment |
| title_short | Tailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment |
| title_sort | tailored 3d agarose well integrated with human skin equivalents for enhanced skin penetration assessment |
| topic | 3D agarose-well skin penetration human skin equivalents high-performance gels |
| url | https://www.mdpi.com/2310-2861/10/11/691 |
| work_keys_str_mv | AT chaewonwoo tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT jinabyun tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT sunggyushin tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT heeseonyoo tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT sungwoocho tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT donghunlee tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT taezoonpark tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment AT jaehyunjeong tailored3dagarosewellintegratedwithhumanskinequivalentsforenhancedskinpenetrationassessment |