Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC
As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl parabe...
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250135 |
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| author | Yisheng Chen Xingjun Xi |
| author_facet | Yisheng Chen Xingjun Xi |
| author_sort | Yisheng Chen |
| collection | DOAJ |
| description | As a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantification, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1%) and accuracy (spike-recovery rate 76.9%–112.2%). Finally, the suspected result was confirmed by determining its MS fingerprint, further strengthening the reliability of screening. |
| format | Article |
| id | doaj-art-33fbbb5785014dba967aa73059b63b26 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-33fbbb5785014dba967aa73059b63b262025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331602160710.26599/FSHW.2022.9250135Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLCYisheng Chen0Xingjun Xi1College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, ChinaInstitute of Agricultural Food Standardization, China National Institute of Standardization, Beijing 100191, ChinaAs a widely used food preservative, methyl paraben was experimentally evidenced with serious hormonelike adverse effects. Herein, a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice. First, the detectability of the bioautography to the analyte on different layer materials was estimated, revealing that normal silica gel was the best choice. After that, the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices. The separation result was then coupled to the optimized bioautography, enabling instant and straightforward screening of the targeted compound. For accurate quantification, bioluminescent inhibition pattern caused by the analyte was processed by image analysis, giving useful sensitivity (LOD > 16 mg/kg), precision (RSD < 10.1%) and accuracy (spike-recovery rate 76.9%–112.2%). Finally, the suspected result was confirmed by determining its MS fingerprint, further strengthening the reliability of screening.https://www.sciopen.com/article/10.26599/FSHW.2022.9250135high performance thin-layer chromatographybio-screeningbioluminescenceimage analysismethyl paraben |
| spellingShingle | Yisheng Chen Xingjun Xi Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC Food Science and Human Wellness high performance thin-layer chromatography bio-screening bioluminescence image analysis methyl paraben |
| title | Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC |
| title_full | Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC |
| title_fullStr | Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC |
| title_full_unstemmed | Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC |
| title_short | Bio-screening and quantification of methyl paraben in vinegar and coconut juice separated by HPTLC |
| title_sort | bio screening and quantification of methyl paraben in vinegar and coconut juice separated by hptlc |
| topic | high performance thin-layer chromatography bio-screening bioluminescence image analysis methyl paraben |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250135 |
| work_keys_str_mv | AT yishengchen bioscreeningandquantificationofmethylparabeninvinegarandcoconutjuiceseparatedbyhptlc AT xingjunxi bioscreeningandquantificationofmethylparabeninvinegarandcoconutjuiceseparatedbyhptlc |