Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities
The complex structure of the plant cell wall makes it difficult to use the biomass produced by biosynthesis. For this reason, the search for new strains of microorganisms capable of efficiently degrading fiber is a topic of interest. For these reasons, the present study aimed to evaluate both the mi...
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2024-11-01
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| Series: | Journal of Fungi |
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| author | Maryen Alberto Vazquez Luis Rodrigo Saa Elaine Valiño Livio Torta Vito Armando Laudicina |
| author_facet | Maryen Alberto Vazquez Luis Rodrigo Saa Elaine Valiño Livio Torta Vito Armando Laudicina |
| author_sort | Maryen Alberto Vazquez |
| collection | DOAJ |
| description | The complex structure of the plant cell wall makes it difficult to use the biomass produced by biosynthesis. For this reason, the search for new strains of microorganisms capable of efficiently degrading fiber is a topic of interest. For these reasons, the present study aimed to evaluate both the microbiological and enzymatic characteristics of the fungus <i>Curvularia kusanoi</i> L7strain. For this, its growth in different culture media was evaluated. Wheat straw mineralization was evaluated by gas chromatography assisted by infrared spectroscopy. The production of endo- and exoglucanase, laccase, and peroxidase enzymes in submerged solid fermentation of wheat and sugarcane bagasse were characterized. The strain efficiently mineralized raw wheat straw, showing a significant decrease in signals associated with cellulose, hemicellulose, and lignin in the infrared spectra. High enzyme productions were achieved in submerged solid fermentation of both substrates, highlighting the high production of endoglucanases in sugarcane bagasse (2.87 IU/mL) and laccases in wheat (1.64 IU/mL). It is concluded that <i>C. kusanoi</i> L7 is an ascomycete with a versatile enzyme production that allows it to exhaustively degrade complex fibers such as raw wheat straw and sugar cane bagasse, making it a microorganism with great potential in the bioconversion of plant biomass. |
| format | Article |
| id | doaj-art-33f0a28478814a638cf5f38bcf0da0c9 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-33f0a28478814a638cf5f38bcf0da0c92024-12-27T14:33:48ZengMDPI AGJournal of Fungi2309-608X2024-11-01101280710.3390/jof10120807Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation PotentialitiesMaryen Alberto Vazquez0Luis Rodrigo Saa1Elaine Valiño2Livio Torta3Vito Armando Laudicina4Institute of Animal Science (ICA), San José de Las Lajas 32700, CubaLaboratorio de Sanidad Animal y Zoonosis, Departmento de Ciencias Biologicas y Agropecuria, Escuela de Ingeniería Agropecuaria, Universidad Técnica Particular de Loja, Loja 1101608, EcuadorInstitute of Animal Science (ICA), San José de Las Lajas 32700, CubaDipartmento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, 90133 Palermo, ItalyDipartmento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, 90133 Palermo, ItalyThe complex structure of the plant cell wall makes it difficult to use the biomass produced by biosynthesis. For this reason, the search for new strains of microorganisms capable of efficiently degrading fiber is a topic of interest. For these reasons, the present study aimed to evaluate both the microbiological and enzymatic characteristics of the fungus <i>Curvularia kusanoi</i> L7strain. For this, its growth in different culture media was evaluated. Wheat straw mineralization was evaluated by gas chromatography assisted by infrared spectroscopy. The production of endo- and exoglucanase, laccase, and peroxidase enzymes in submerged solid fermentation of wheat and sugarcane bagasse were characterized. The strain efficiently mineralized raw wheat straw, showing a significant decrease in signals associated with cellulose, hemicellulose, and lignin in the infrared spectra. High enzyme productions were achieved in submerged solid fermentation of both substrates, highlighting the high production of endoglucanases in sugarcane bagasse (2.87 IU/mL) and laccases in wheat (1.64 IU/mL). It is concluded that <i>C. kusanoi</i> L7 is an ascomycete with a versatile enzyme production that allows it to exhaustively degrade complex fibers such as raw wheat straw and sugar cane bagasse, making it a microorganism with great potential in the bioconversion of plant biomass.https://www.mdpi.com/2309-608X/10/12/807fungicell wall degrading enzymescarbon mineralizationfiber modification |
| spellingShingle | Maryen Alberto Vazquez Luis Rodrigo Saa Elaine Valiño Livio Torta Vito Armando Laudicina Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities Journal of Fungi fungi cell wall degrading enzymes carbon mineralization fiber modification |
| title | Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities |
| title_full | Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities |
| title_fullStr | Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities |
| title_full_unstemmed | Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities |
| title_short | Microbiological Aspects and Enzymatic Characterization of <i>Curvularia kusanoi</i> L7: Ascomycete with Great Biomass Degradation Potentialities |
| title_sort | microbiological aspects and enzymatic characterization of i curvularia kusanoi i l7 ascomycete with great biomass degradation potentialities |
| topic | fungi cell wall degrading enzymes carbon mineralization fiber modification |
| url | https://www.mdpi.com/2309-608X/10/12/807 |
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