Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study
<b>Background/Objective</b>: Anti-tumor effects of plasma-activated medium (PAM) were demonstrated using various malignant tumors. However, the anti-tumor effect of PAM on synovial sarcoma remains unclear. Therefore, we aimed to investigate the anti-tumor effects of PAM on synovial sarco...
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2025-02-01
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| author | Hana Yao Hiromitsu Toyoda Naoki Takada Naoto Oebisu Kumi Orita Yoshitaka Ban Kosuke Saito Katsumasa Nakazawa Yuto Kobayashi Hiroshi Taniwaki Chinatsu Ohira Jun-Seok Oh Tatsuru Shirafuji Hidetomi Terai Hiroaki Nakamura |
| author_facet | Hana Yao Hiromitsu Toyoda Naoki Takada Naoto Oebisu Kumi Orita Yoshitaka Ban Kosuke Saito Katsumasa Nakazawa Yuto Kobayashi Hiroshi Taniwaki Chinatsu Ohira Jun-Seok Oh Tatsuru Shirafuji Hidetomi Terai Hiroaki Nakamura |
| author_sort | Hana Yao |
| collection | DOAJ |
| description | <b>Background/Objective</b>: Anti-tumor effects of plasma-activated medium (PAM) were demonstrated using various malignant tumors. However, the anti-tumor effect of PAM on synovial sarcoma remains unclear. Therefore, we aimed to investigate the anti-tumor effects of PAM on synovial sarcoma and its underlying mechanisms, focusing on the quantitative analyses of both intracellular reactive oxygen species (ROS) and cell apoptosis. <b>Methods</b>: The human synovial sarcoma cell line HS-SY-II was used to investigate the cell viability after PAM treatment. We investigated the anti-tumor effects and side effects of local PAM injection in a synovial sarcoma xenograft murine model. Moreover, we observed PAM-induced intracellular ROS accumulation and cell apoptosis and assessed the involvement of intracellular ROS in the anti-tumor effects of PAM using an intracellular ROS scavenger. <b>Results</b>: PAM significantly decreased the viability of synovial sarcoma cells compared with untreated Dulbecco’s modified Eagle medium. Local PAM injection into a synovial sarcoma xenograft murine model significantly suppressed tumor growth, including tumor volume (<i>p</i> < 0.001) and weight (<i>p</i> = 0.031), without side effects. Regarding anti-tumor mechanisms, PAM induced significant cell apoptosis and intracellular ROS accumulation (<i>p</i> < 0.001). The intracellular ROS scavenger significantly inhibited the anti-tumor effect of PAM (<i>p</i> < 0.001). <b>Conclusions</b>: We confirmed the anti-tumor effects of PAM on synovial sarcoma in vitro and in vivo, as well as the absence of side effects. The underlying mechanism was suggested to involve cell apoptosis induced by intracellular ROS accumulation. Considering the various clinical issues associated with the existing treatments of synovial sarcoma, PAM is a promising new option. |
| format | Article |
| id | doaj-art-33e86a5dfd9b4176ad92a8c5d24cec9a |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj-art-33e86a5dfd9b4176ad92a8c5d24cec9a2025-08-20T03:43:02ZengMDPI AGBiomedicines2227-90592025-02-0113353410.3390/biomedicines13030534Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo StudyHana Yao0Hiromitsu Toyoda1Naoki Takada2Naoto Oebisu3Kumi Orita4Yoshitaka Ban5Kosuke Saito6Katsumasa Nakazawa7Yuto Kobayashi8Hiroshi Taniwaki9Chinatsu Ohira10Jun-Seok Oh11Tatsuru Shirafuji12Hidetomi Terai13Hiroaki Nakamura14Department of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Osaka City Juso Hospital, Osaka 532-0034, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Physics and Electronics, Graduate School of Engineering, Osaka Metropolitan University, Osaka 558-8585, JapanDepartment of Physics and Electronics, Graduate School of Engineering, Osaka Metropolitan University, Osaka 558-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDepartment of Orthopaedic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, Japan<b>Background/Objective</b>: Anti-tumor effects of plasma-activated medium (PAM) were demonstrated using various malignant tumors. However, the anti-tumor effect of PAM on synovial sarcoma remains unclear. Therefore, we aimed to investigate the anti-tumor effects of PAM on synovial sarcoma and its underlying mechanisms, focusing on the quantitative analyses of both intracellular reactive oxygen species (ROS) and cell apoptosis. <b>Methods</b>: The human synovial sarcoma cell line HS-SY-II was used to investigate the cell viability after PAM treatment. We investigated the anti-tumor effects and side effects of local PAM injection in a synovial sarcoma xenograft murine model. Moreover, we observed PAM-induced intracellular ROS accumulation and cell apoptosis and assessed the involvement of intracellular ROS in the anti-tumor effects of PAM using an intracellular ROS scavenger. <b>Results</b>: PAM significantly decreased the viability of synovial sarcoma cells compared with untreated Dulbecco’s modified Eagle medium. Local PAM injection into a synovial sarcoma xenograft murine model significantly suppressed tumor growth, including tumor volume (<i>p</i> < 0.001) and weight (<i>p</i> = 0.031), without side effects. Regarding anti-tumor mechanisms, PAM induced significant cell apoptosis and intracellular ROS accumulation (<i>p</i> < 0.001). The intracellular ROS scavenger significantly inhibited the anti-tumor effect of PAM (<i>p</i> < 0.001). <b>Conclusions</b>: We confirmed the anti-tumor effects of PAM on synovial sarcoma in vitro and in vivo, as well as the absence of side effects. The underlying mechanism was suggested to involve cell apoptosis induced by intracellular ROS accumulation. Considering the various clinical issues associated with the existing treatments of synovial sarcoma, PAM is a promising new option.https://www.mdpi.com/2227-9059/13/3/534anti-tumor effectcell apoptosisintracellular reactive oxygen species (ROS)non-thermal atmospheric pressure plasmaplasma-activated medium (PAM)synovial sarcoma |
| spellingShingle | Hana Yao Hiromitsu Toyoda Naoki Takada Naoto Oebisu Kumi Orita Yoshitaka Ban Kosuke Saito Katsumasa Nakazawa Yuto Kobayashi Hiroshi Taniwaki Chinatsu Ohira Jun-Seok Oh Tatsuru Shirafuji Hidetomi Terai Hiroaki Nakamura Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study Biomedicines anti-tumor effect cell apoptosis intracellular reactive oxygen species (ROS) non-thermal atmospheric pressure plasma plasma-activated medium (PAM) synovial sarcoma |
| title | Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study |
| title_full | Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study |
| title_fullStr | Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study |
| title_full_unstemmed | Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study |
| title_short | Anti-Tumor Effect of Non-Thermal Atmospheric Pressure Plasma-Activated Medium on Synovial Sarcoma: An In Vitro and In Vivo Study |
| title_sort | anti tumor effect of non thermal atmospheric pressure plasma activated medium on synovial sarcoma an in vitro and in vivo study |
| topic | anti-tumor effect cell apoptosis intracellular reactive oxygen species (ROS) non-thermal atmospheric pressure plasma plasma-activated medium (PAM) synovial sarcoma |
| url | https://www.mdpi.com/2227-9059/13/3/534 |
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