Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i>
The cultivation of <i>Fritillaria ussuriensis</i> faces challenges due to the prevalent <i>Fritillaria</i> wilt disease, hindering large-scale production. To address this, we aimed to understand the disease’s characteristics and develop effective prevention measures. Microbia...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/13/11/940 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846154231516495872 |
|---|---|
| author | Hao Wu Jingjing Lu Simeng Zhao Jingyi Fei Zhimiao Qu Min Zhao Hongyan Yang |
| author_facet | Hao Wu Jingjing Lu Simeng Zhao Jingyi Fei Zhimiao Qu Min Zhao Hongyan Yang |
| author_sort | Hao Wu |
| collection | DOAJ |
| description | The cultivation of <i>Fritillaria ussuriensis</i> faces challenges due to the prevalent <i>Fritillaria</i> wilt disease, hindering large-scale production. To address this, we aimed to understand the disease’s characteristics and develop effective prevention measures. Microbial communities of diseased <i>F. ussuriensis</i> plants were analyzed, pathogenic and antagonistic strains were screened, and biocontrol feasibility was tested. We identified <i>Botryotinia</i> predominance in aboveground parts and variations in <i>Mrakia</i>, <i>Humicola</i>, <i>llyonectria</i>, and <i>Fusarium</i> in underground parts. The pathogens <i>Fusarium oxysporum</i> IFM-1 and <i>Fusarium solani</i> IFM-52 isolated from diseased <i>F. ussuriensis</i> not only caused severe <i>Fritillaria</i> wilt but were also pathogenic to <i>Lilium lancifolium</i> and <i>Allium cepa</i> var. <i>aggregatum</i> in Liliaceae. The antagonistic <i>Bacillus tequilensis</i> LFM-30 and <i>Trichoderma koningiopsis</i> IFM-47 isolated from diseased plants significantly alleviated plant wilt and showed promise in preventing wilt disease caused by <i>Fusarium</i> in Liliaceae plants. Our study highlights distinct microbial differences between healthy and diseased <i>F. ussuriensis</i> and underscores the pathogenicity of <i>Fusarium</i>. Using <i>T. koningiopsis</i> and <i>B. tequilensis</i> either singly or in combination could offer effective biocontrol against <i>F. solani</i> and <i>F. oxysporum</i>, benefiting <i>F. ussuriensis</i> and related Liliaceae plants. |
| format | Article |
| id | doaj-art-33b711e7042a4574acb8d18a87feb89a |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-33b711e7042a4574acb8d18a87feb89a2024-11-26T17:52:32ZengMDPI AGBiology2079-77372024-11-01131194010.3390/biology13110940Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i>Hao Wu0Jingjing Lu1Simeng Zhao2Jingyi Fei3Zhimiao Qu4Min Zhao5Hongyan Yang6College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaThe cultivation of <i>Fritillaria ussuriensis</i> faces challenges due to the prevalent <i>Fritillaria</i> wilt disease, hindering large-scale production. To address this, we aimed to understand the disease’s characteristics and develop effective prevention measures. Microbial communities of diseased <i>F. ussuriensis</i> plants were analyzed, pathogenic and antagonistic strains were screened, and biocontrol feasibility was tested. We identified <i>Botryotinia</i> predominance in aboveground parts and variations in <i>Mrakia</i>, <i>Humicola</i>, <i>llyonectria</i>, and <i>Fusarium</i> in underground parts. The pathogens <i>Fusarium oxysporum</i> IFM-1 and <i>Fusarium solani</i> IFM-52 isolated from diseased <i>F. ussuriensis</i> not only caused severe <i>Fritillaria</i> wilt but were also pathogenic to <i>Lilium lancifolium</i> and <i>Allium cepa</i> var. <i>aggregatum</i> in Liliaceae. The antagonistic <i>Bacillus tequilensis</i> LFM-30 and <i>Trichoderma koningiopsis</i> IFM-47 isolated from diseased plants significantly alleviated plant wilt and showed promise in preventing wilt disease caused by <i>Fusarium</i> in Liliaceae plants. Our study highlights distinct microbial differences between healthy and diseased <i>F. ussuriensis</i> and underscores the pathogenicity of <i>Fusarium</i>. Using <i>T. koningiopsis</i> and <i>B. tequilensis</i> either singly or in combination could offer effective biocontrol against <i>F. solani</i> and <i>F. oxysporum</i>, benefiting <i>F. ussuriensis</i> and related Liliaceae plants.https://www.mdpi.com/2079-7737/13/11/940<i>Fritillaria</i>wiltmicrobial community<i>Bacillus</i><i>Trichoderma</i> |
| spellingShingle | Hao Wu Jingjing Lu Simeng Zhao Jingyi Fei Zhimiao Qu Min Zhao Hongyan Yang Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> Biology <i>Fritillaria</i> wilt microbial community <i>Bacillus</i> <i>Trichoderma</i> |
| title | Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> |
| title_full | Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> |
| title_fullStr | Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> |
| title_full_unstemmed | Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> |
| title_short | Microbial Community of Wilted <i>Fritillaria ussuriensis</i> and Biocontrol Effects of <i>Bacillus tequilensis</i> and <i>Trichoderma koningiopsis</i> |
| title_sort | microbial community of wilted i fritillaria ussuriensis i and biocontrol effects of i bacillus tequilensis i and i trichoderma koningiopsis i |
| topic | <i>Fritillaria</i> wilt microbial community <i>Bacillus</i> <i>Trichoderma</i> |
| url | https://www.mdpi.com/2079-7737/13/11/940 |
| work_keys_str_mv | AT haowu microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT jingjinglu microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT simengzhao microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT jingyifei microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT zhimiaoqu microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT minzhao microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi AT hongyanyang microbialcommunityofwiltedifritillariaussuriensisiandbiocontroleffectsofibacillustequilensisianditrichodermakoningiopsisi |