TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients
Thymic stromal lymphopoietin (TSLP) has been identified as a crucial inflammatory cytokine in immune homeostasis. Previous studies have reported conflicting effects of TSLP on sepsis in mice, and the effect of TSLP on sepsis in humans has not been investigated. In this study, we used the ELISA to me...
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Wiley
2019-12-01
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| Online Access: | https://doi.org/10.1002/2211-5463.12746 |
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| author | Qichun Yu Yang Li Hao Wang Huawei Xiong |
| author_facet | Qichun Yu Yang Li Hao Wang Huawei Xiong |
| author_sort | Qichun Yu |
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| description | Thymic stromal lymphopoietin (TSLP) has been identified as a crucial inflammatory cytokine in immune homeostasis. Previous studies have reported conflicting effects of TSLP on sepsis in mice, and the effect of TSLP on sepsis in humans has not been investigated. In this study, we used the ELISA to measure serum levels of TSLP in patients with sepsis, and used flow cytometry and ELISA to evaluate the proinflammatory phenotype of circulating immune cells. In addition, we used quantitative RT‐PCR to examine the expression of proinflammatory cytokines [interleukin (IL)‐1β, IL‐6, tumor necrosis factor‐α, transferrin growth factor‐β, IL‐10, and matrix metalloproteinase] between patients with high and low levels of TSLP. Flow cytometry analysis was performed to evaluate the phagocytic and respiratory burst of circulating neutrophils. A significant increase in the production of proinflammatory cytokines by nonclassical monocytes and the number of interferon (IFN)‐γ+ CD4+ monocytes was observed in patients with high levels of TSLP. Furthermore, the number of IL‐10+ regulatory T cells was observed to be increased in patients with high levels of TSLP. We found that TSLP values greater than 350 pg·mL−1 were associated with a higher mortality rate and longer stays in intensive care (sensitivity of 89% and specificity of 79%). In patients with low levels of neutrophils, the area under curve was only 0.71 (based on the cutoff value in the diagnostic test evaluation; sensitivity of 62% and specificity of 68%). Our findings suggest that the serum levels of TSLP may be suitable as a biomarker for prediction of prognosis in a subgroup of patients with sepsis who are exhibiting hyperleukocytosis and a high neutrophil ratio. |
| format | Article |
| id | doaj-art-33ac68f62d4c41968e5e868cbb6e57d1 |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2019-12-01 |
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| spelling | doaj-art-33ac68f62d4c41968e5e868cbb6e57d12024-12-06T11:26:36ZengWileyFEBS Open Bio2211-54632019-12-019122137214810.1002/2211-5463.12746TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patientsQichun Yu0Yang Li1Hao Wang2Huawei Xiong3Department of Orthopedics The First Affiliated Hospital of Nanchang University ChinaDepartment of Emergency The First Affiliated Hospital of Nanchang University ChinaDepartment of Emergency The First Affiliated Hospital of Nanchang University ChinaDepartment of Emergency The First Affiliated Hospital of Nanchang University ChinaThymic stromal lymphopoietin (TSLP) has been identified as a crucial inflammatory cytokine in immune homeostasis. Previous studies have reported conflicting effects of TSLP on sepsis in mice, and the effect of TSLP on sepsis in humans has not been investigated. In this study, we used the ELISA to measure serum levels of TSLP in patients with sepsis, and used flow cytometry and ELISA to evaluate the proinflammatory phenotype of circulating immune cells. In addition, we used quantitative RT‐PCR to examine the expression of proinflammatory cytokines [interleukin (IL)‐1β, IL‐6, tumor necrosis factor‐α, transferrin growth factor‐β, IL‐10, and matrix metalloproteinase] between patients with high and low levels of TSLP. Flow cytometry analysis was performed to evaluate the phagocytic and respiratory burst of circulating neutrophils. A significant increase in the production of proinflammatory cytokines by nonclassical monocytes and the number of interferon (IFN)‐γ+ CD4+ monocytes was observed in patients with high levels of TSLP. Furthermore, the number of IL‐10+ regulatory T cells was observed to be increased in patients with high levels of TSLP. We found that TSLP values greater than 350 pg·mL−1 were associated with a higher mortality rate and longer stays in intensive care (sensitivity of 89% and specificity of 79%). In patients with low levels of neutrophils, the area under curve was only 0.71 (based on the cutoff value in the diagnostic test evaluation; sensitivity of 62% and specificity of 68%). Our findings suggest that the serum levels of TSLP may be suitable as a biomarker for prediction of prognosis in a subgroup of patients with sepsis who are exhibiting hyperleukocytosis and a high neutrophil ratio.https://doi.org/10.1002/2211-5463.12746cytokineproinflammatory phenotypesepsisthymic stromal lymphopoietinTSLP |
| spellingShingle | Qichun Yu Yang Li Hao Wang Huawei Xiong TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients FEBS Open Bio cytokine proinflammatory phenotype sepsis thymic stromal lymphopoietin TSLP |
| title | TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| title_full | TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| title_fullStr | TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| title_full_unstemmed | TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| title_short | TSLP induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| title_sort | tslp induces a proinflammatory phenotype in circulating innate cells and predicts prognosis in sepsis patients |
| topic | cytokine proinflammatory phenotype sepsis thymic stromal lymphopoietin TSLP |
| url | https://doi.org/10.1002/2211-5463.12746 |
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