Protocol for analyzing the function of the type VI secretion system of the oral symbiont Aggregatibacter aphrophilus in targeting pathobionts

Protocol for analyzing the function of the type VI secretion system of the oral symbiont Aggregatibacter aphrophilus in targeting pathobionts

Summary: Here, we present a protocol for evaluating type VI secretion system (T6SS)-dependent fitness of the oral symbiont A. aphrophilus using biofilm competition assays and metaproteomics. We describe steps for designing T6SS-specific mutants. We then detail procedures for using them in competitio...

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Bibliographic Details
Main Authors: Jan Oscarsson, Kai Bao, Akiko Shiratsuchi, Jonas Grossmann, Witold Wolski, Kyaw Min Aung, Mark Lindholm, Anders Johansson, Ferdousi Rahman Mowsumi, Sun Nyunt Wai, Georgios N. Belibasakis, Nagihan Bostanci
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
genomics
microbiology
molecular biology
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400580X
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