Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats
Aim: This study aimed to evaluate the therapeutic efficacy of Trolox and neocuproine treatment in an experimental rat model of mild traumatic brain injury (mTBI).Material and Methods: Forty rats were grouped as Control, TBI, Trolox, and neocuproine. After the Marmarou Weight Drop Model was used to c...
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Duzce University
2024-12-01
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| Series: | Düzce Tıp Fakültesi Dergisi |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/4267547 |
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| author | Fatma Yesilyurt Güven Akçay Selma Yaman |
| author_facet | Fatma Yesilyurt Güven Akçay Selma Yaman |
| author_sort | Fatma Yesilyurt |
| collection | DOAJ |
| description | Aim: This study aimed to evaluate the therapeutic efficacy of Trolox and neocuproine treatment in an experimental rat model of mild traumatic brain injury (mTBI).Material and Methods: Forty rats were grouped as Control, TBI, Trolox, and neocuproine. After the Marmarou Weight Drop Model was used to create TBI, 80 mg/kg/day Trolox (i.p.), and 100 μM/day Neocuproine (i.p.) treatments were applied in the 2nd hour. Open field, attention, and short-term memory novel object recognition tests were performed to measure locomotor activity. IL-1β, IL-10, TNF-α, and TGF-β levels in the hippocampus tissues of the rats were analyzed by the ELISA method.Results: Open field and novel object recognition test results showed that the total path traveled was significantly decreased in the TBI group compared to the control group (p<0.001). A significant increase in locomotor functions was observed in the Trolox (p=0.018) and neocuproine (p=0.002) groups compared to TBI. Short-term memory test results decreased in TBI compared to control (p<0.001), while they increased in the Trolox and neocuproine groups compared to TBI (both p<0.001). Hippocampus IL-1β (p=0.012) and TNF-α (p=0.011) levels increased, while IL-10 (p=0.031) and TGF-β (p=0.007) levels decreased compared to control in the TBI group. While all proinflammatory cytokine levels decreased, antiinflammatory cytokine levels increased in the Trolox and neocuproine groups compared to TBI.Conclusion: The present findings showed that Trolox and neocuproine treatments in TBI provided significant improvements in short-term memory, and locomotor activity levels by reducing proinflammatory cytokine production, and increasing antiinflammatory cytokine production. |
| format | Article |
| id | doaj-art-32f3f8c556a545e693e4ea72b85ae761 |
| institution | Kabale University |
| issn | 1307-671X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Duzce University |
| record_format | Article |
| series | Düzce Tıp Fakültesi Dergisi |
| spelling | doaj-art-32f3f8c556a545e693e4ea72b85ae7612024-12-25T18:31:09ZengDuzce UniversityDüzce Tıp Fakültesi Dergisi1307-671X2024-12-0126326326810.18678/dtfd.156233497Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in RatsFatma Yesilyurt0https://orcid.org/0000-0002-1336-6322Güven Akçay1https://orcid.org/0000-0003-3418-8825Selma Yaman2https://orcid.org/0000-0002-9301-9119ATATURK UNIVERSITYBOLU ABANT IZZET BAYSAL UNIVERSITYKahramanmaraş Sütçü İmam Ünv. Tıp Fak. Biyofizik ADAim: This study aimed to evaluate the therapeutic efficacy of Trolox and neocuproine treatment in an experimental rat model of mild traumatic brain injury (mTBI).Material and Methods: Forty rats were grouped as Control, TBI, Trolox, and neocuproine. After the Marmarou Weight Drop Model was used to create TBI, 80 mg/kg/day Trolox (i.p.), and 100 μM/day Neocuproine (i.p.) treatments were applied in the 2nd hour. Open field, attention, and short-term memory novel object recognition tests were performed to measure locomotor activity. IL-1β, IL-10, TNF-α, and TGF-β levels in the hippocampus tissues of the rats were analyzed by the ELISA method.Results: Open field and novel object recognition test results showed that the total path traveled was significantly decreased in the TBI group compared to the control group (p<0.001). A significant increase in locomotor functions was observed in the Trolox (p=0.018) and neocuproine (p=0.002) groups compared to TBI. Short-term memory test results decreased in TBI compared to control (p<0.001), while they increased in the Trolox and neocuproine groups compared to TBI (both p<0.001). Hippocampus IL-1β (p=0.012) and TNF-α (p=0.011) levels increased, while IL-10 (p=0.031) and TGF-β (p=0.007) levels decreased compared to control in the TBI group. While all proinflammatory cytokine levels decreased, antiinflammatory cytokine levels increased in the Trolox and neocuproine groups compared to TBI.Conclusion: The present findings showed that Trolox and neocuproine treatments in TBI provided significant improvements in short-term memory, and locomotor activity levels by reducing proinflammatory cytokine production, and increasing antiinflammatory cytokine production.https://dergipark.org.tr/en/download/article-file/4267547neokuprintrolokstravmatik beyin hasarılokomotor aktivitenöroinflamasyonneocuproinetroloxtraumatic brain injurylocomotor activityneuroinflammation |
| spellingShingle | Fatma Yesilyurt Güven Akçay Selma Yaman Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats Düzce Tıp Fakültesi Dergisi neokuprin troloks travmatik beyin hasarı lokomotor aktivite nöroinflamasyon neocuproine trolox traumatic brain injury locomotor activity neuroinflammation |
| title | Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats |
| title_full | Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats |
| title_fullStr | Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats |
| title_full_unstemmed | Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats |
| title_short | Therapeutic Effects of Trolox and Neocuproine on Experimental Mild Traumatic Brain Injury in Rats |
| title_sort | therapeutic effects of trolox and neocuproine on experimental mild traumatic brain injury in rats |
| topic | neokuprin troloks travmatik beyin hasarı lokomotor aktivite nöroinflamasyon neocuproine trolox traumatic brain injury locomotor activity neuroinflammation |
| url | https://dergipark.org.tr/en/download/article-file/4267547 |
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