Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane.
Simian Virus 40 (SV40) is a paradigm pathogen with multivalent binding sites for the sphingolipid GM1, via which it induces its endocytosis for infection. Here we report that SV40 also utilizes cell surface integrins to activate signaling networks required for infection, even in the absence of the p...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0055799&type=printable |
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| author | Lilli Stergiou Manuel Bauer Waltraud Mair Damaris Bausch-Fluck Nir Drayman Bernd Wollscheid Ariella Oppenheim Lucas Pelkmans |
| author_facet | Lilli Stergiou Manuel Bauer Waltraud Mair Damaris Bausch-Fluck Nir Drayman Bernd Wollscheid Ariella Oppenheim Lucas Pelkmans |
| author_sort | Lilli Stergiou |
| collection | DOAJ |
| description | Simian Virus 40 (SV40) is a paradigm pathogen with multivalent binding sites for the sphingolipid GM1, via which it induces its endocytosis for infection. Here we report that SV40 also utilizes cell surface integrins to activate signaling networks required for infection, even in the absence of the previously implicated glycosphingolipids. We identify ILK, PDK1, the RhoGAP GRAF1 and RhoA as core nodes of the signaling network activated upon SV40 engagement of integrins. We show that integrin-mediated signaling through host SV40 engagement induces the de-phosphorylation of Ezrin leading to uncoupling of the plasma membrane and cortical actin. Our results provide functional evidence for a mechanism by which SV40 activates signal transduction in human epithelial cells via integrins in the context of clathrin-independent endocytosis. |
| format | Article |
| id | doaj-art-3273076133e1463f9451c3ba1a548437 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-3273076133e1463f9451c3ba1a5484372025-01-16T05:31:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5579910.1371/journal.pone.0055799Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane.Lilli StergiouManuel BauerWaltraud MairDamaris Bausch-FluckNir DraymanBernd WollscheidAriella OppenheimLucas PelkmansSimian Virus 40 (SV40) is a paradigm pathogen with multivalent binding sites for the sphingolipid GM1, via which it induces its endocytosis for infection. Here we report that SV40 also utilizes cell surface integrins to activate signaling networks required for infection, even in the absence of the previously implicated glycosphingolipids. We identify ILK, PDK1, the RhoGAP GRAF1 and RhoA as core nodes of the signaling network activated upon SV40 engagement of integrins. We show that integrin-mediated signaling through host SV40 engagement induces the de-phosphorylation of Ezrin leading to uncoupling of the plasma membrane and cortical actin. Our results provide functional evidence for a mechanism by which SV40 activates signal transduction in human epithelial cells via integrins in the context of clathrin-independent endocytosis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0055799&type=printable |
| spellingShingle | Lilli Stergiou Manuel Bauer Waltraud Mair Damaris Bausch-Fluck Nir Drayman Bernd Wollscheid Ariella Oppenheim Lucas Pelkmans Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. PLoS ONE |
| title | Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. |
| title_full | Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. |
| title_fullStr | Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. |
| title_full_unstemmed | Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. |
| title_short | Integrin-mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane. |
| title_sort | integrin mediated signaling induced by simian virus 40 leads to transient uncoupling of cortical actin and the plasma membrane |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0055799&type=printable |
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