The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity
Objective: Aquaporins (AQPs) are a large family of proteins that help transport water and small molecules. AQP7 is an AQP responsible for the extracellular transport of glycerol produced by lipolysis in adipocytes. Perilipin 1 (PLIN1) regulates lipolysis on the surface of lipid droplets in adipocyte...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2024-12-01
|
| Series: | Turkish Journal of Immunology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-93063 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841525604117118976 |
|---|---|
| author | Jülide Tozkır Nihayet Fırat Ebru Göncü Onur Ersoy Pınar Tayfur Orkide Palabıyık |
| author_facet | Jülide Tozkır Nihayet Fırat Ebru Göncü Onur Ersoy Pınar Tayfur Orkide Palabıyık |
| author_sort | Jülide Tozkır |
| collection | DOAJ |
| description | Objective: Aquaporins (AQPs) are a large family of proteins that help transport water and small molecules. AQP7 is an AQP responsible for the extracellular transport of glycerol produced by lipolysis in adipocytes. Perilipin 1 (PLIN1) regulates lipolysis on the surface of lipid droplets in adipocytes and promotes the transport of glycerol out of the cell via AQP7. It is not known exactly how AQP are regulated in thymic tissue. The goal of this study was to evaluate the expression status of AQP7 and PLIN1 in thymic tissue during thymic involution and to determine how these expression patterns are affected by high-fructose diet and voluntary physical activity.
Materials and Methods: In this study, 18 adult female Sprague-Dawley rats were assigned to three groups: control (C), fructose (F), and fructose + physical activity (FA). Fructose was added at 20% to the drinking water of the F and FA groups. At the end of 8 weeks, rats were euthanized under appropriate conditions, and tissue and blood samples were collected. Histological evaluation of the thymus was performed by hematoxylin-eosin staining. The expression levels of AQP7 and PLIN1 were determined real-time reverse transcription polymerase chain reaction using appropriate primers.
Results: The weight of the thymus tissue decreased in the FA group compared with that in the F group due to exercise (p=0.015), but the cortex and medulla structure were histologically preserved. AQP7 was significantly decreased in the F group compared with the C and FA groups (p=0.0079 and p=0.0127, respectively).
Conclusion: AQPs and their associated molecules can be strategic targets for slowing or reversing thymic involution. |
| format | Article |
| id | doaj-art-3238594a975a4dd28f4a192345fc1cca |
| institution | Kabale University |
| issn | 1301-109X 2147-8325 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Immunology |
| spelling | doaj-art-3238594a975a4dd28f4a192345fc1cca2025-01-17T08:29:35ZengGalenos Publishing HouseTurkish Journal of Immunology1301-109X2147-83252024-12-0112310611310.4274/tji.galenos.2024.93063TJI-93063The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical ActivityJülide Tozkır0Nihayet Fırat1Ebru Göncü2Onur Ersoy3Pınar Tayfur4Orkide Palabıyık5Trakya University Vocational School of Health Services, Edirne, TurkeyTrakya University Institute of Health Sciences, Department of Physiology, Edirne, TurkeyTrakya University Medical Research and Application Centre, Department of Medical Genetics, Edirne, TurkeyTrakya University Vocational School of Health Services, Edirne, TurkeyBahçeşehir University Faculty of Health Science, İstanbul, TurkeyTrakya University Vocational School of Health Services, Edirne, TurkeyObjective: Aquaporins (AQPs) are a large family of proteins that help transport water and small molecules. AQP7 is an AQP responsible for the extracellular transport of glycerol produced by lipolysis in adipocytes. Perilipin 1 (PLIN1) regulates lipolysis on the surface of lipid droplets in adipocytes and promotes the transport of glycerol out of the cell via AQP7. It is not known exactly how AQP are regulated in thymic tissue. The goal of this study was to evaluate the expression status of AQP7 and PLIN1 in thymic tissue during thymic involution and to determine how these expression patterns are affected by high-fructose diet and voluntary physical activity. Materials and Methods: In this study, 18 adult female Sprague-Dawley rats were assigned to three groups: control (C), fructose (F), and fructose + physical activity (FA). Fructose was added at 20% to the drinking water of the F and FA groups. At the end of 8 weeks, rats were euthanized under appropriate conditions, and tissue and blood samples were collected. Histological evaluation of the thymus was performed by hematoxylin-eosin staining. The expression levels of AQP7 and PLIN1 were determined real-time reverse transcription polymerase chain reaction using appropriate primers. Results: The weight of the thymus tissue decreased in the FA group compared with that in the F group due to exercise (p=0.015), but the cortex and medulla structure were histologically preserved. AQP7 was significantly decreased in the F group compared with the C and FA groups (p=0.0079 and p=0.0127, respectively). Conclusion: AQPs and their associated molecules can be strategic targets for slowing or reversing thymic involution.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-93063thymusaquaglyceroporin 7perilipin 1fructosevoluntary physical activity |
| spellingShingle | Jülide Tozkır Nihayet Fırat Ebru Göncü Onur Ersoy Pınar Tayfur Orkide Palabıyık The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity Turkish Journal of Immunology thymus aquaglyceroporin 7 perilipin 1 fructose voluntary physical activity |
| title | The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity |
| title_full | The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity |
| title_fullStr | The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity |
| title_full_unstemmed | The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity |
| title_short | The Expression of Thymic AQP7 and Perilipin 1 (PLIN1) in Rats Fed a High-Fructose Diet is Modified by Voluntary Physical Activity |
| title_sort | expression of thymic aqp7 and perilipin 1 plin1 in rats fed a high fructose diet is modified by voluntary physical activity |
| topic | thymus aquaglyceroporin 7 perilipin 1 fructose voluntary physical activity |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tji&un=TJI-93063 |
| work_keys_str_mv | AT julidetozkır theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT nihayetfırat theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT ebrugoncu theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT onurersoy theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT pınartayfur theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT orkidepalabıyık theexpressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT julidetozkır expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT nihayetfırat expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT ebrugoncu expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT onurersoy expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT pınartayfur expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity AT orkidepalabıyık expressionofthymicaqp7andperilipin1plin1inratsfedahighfructosedietismodifiedbyvoluntaryphysicalactivity |