Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer

Colorectal cancer (CRC) has a postoperative recurrence rate of up to 50%. Local treatment at the surgical site offers a new strategy to prevent recurrence, minimizing systemic side effects. However, these treatments often face issues like poor stability, insufficient targeting, and significant local...

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Main Authors: Man Zhang, Ke Li, Junhao Kou, Guozhi Lu, Ling Qiu, Chunzhao Yang, Yongchun Liu, Qi Xue, Peng Yang
Format: Article
Language:English
Published: Wiley-VCH 2024-11-01
Series:Small Science
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Online Access:https://doi.org/10.1002/smsc.202400323
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author Man Zhang
Ke Li
Junhao Kou
Guozhi Lu
Ling Qiu
Chunzhao Yang
Yongchun Liu
Qi Xue
Peng Yang
author_facet Man Zhang
Ke Li
Junhao Kou
Guozhi Lu
Ling Qiu
Chunzhao Yang
Yongchun Liu
Qi Xue
Peng Yang
author_sort Man Zhang
collection DOAJ
description Colorectal cancer (CRC) has a postoperative recurrence rate of up to 50%. Local treatment at the surgical site offers a new strategy to prevent recurrence, minimizing systemic side effects. However, these treatments often face issues like poor stability, insufficient targeting, and significant local side effects. Research shows glucose starvation can induce disulfidptosis, a novel cell death pathway, in CRC cells, suitable for precise intervention in residual tumor cells postsurgery. This study develops a biocompatible 2D therapeutic platform using amyloid‐like protein nanofilm technology for precise local synergistic intervention in disulfidptosis and photodynamic therapy (PDT) after CRC surgery. The nanofilm boasts excellent drug‐loading capacity, enzyme activity retention, ultrathin dimensions, wet adhesion, and biocompatibility. A model simulating incomplete tumor resection post‐CRC surgery shows that the GCI@nanofilm effectively inhibits recurrence through an enzyme‐catalyzed cascade reaction inducing disulfidptosis and synergizing with PDT. In summary, GCI@nanofilm overcomes multiple challenges in local postsurgical intervention, providing a foundation for precise and efficient treatment of residual tumors.
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institution Kabale University
issn 2688-4046
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publishDate 2024-11-01
publisher Wiley-VCH
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series Small Science
spelling doaj-art-3236ab98d7ab48b788e42a0a7cfc7b932024-11-11T15:33:39ZengWiley-VCHSmall Science2688-40462024-11-01411n/an/a10.1002/smsc.202400323Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal CancerMan Zhang0Ke Li1Junhao Kou2Guozhi Lu3Ling Qiu4Chunzhao Yang5Yongchun Liu6Qi Xue7Peng Yang8Department of General Surgery Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine Southern Medical University Guangdong 510315 ChinaXi'an Key Laboratory for Prevention and Treatment of Common Aging Diseases Translational and Research Centre for Prevention and Therapy of Chronic Disease Institute of Basic and Translational Medicine Xi'an Medical University Xi'an 710021 ChinaCollege of Pharmacy Xi'an Medical University Xi'an 710021 ChinaCollege of Clinical Medicine Xi'an Medical University Xi'an 710021 ChinaDepartment of General Surgery Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine Southern Medical University Guangdong 510315 ChinaDepartment of General Surgery Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine Southern Medical University Guangdong 510315 ChinaKey Laboratory of Applied Surface and Colloid Chemistry Ministry of Education School of Chemistry and Chemical Engineering Shaanxi Normal University Xi'an 710119 ChinaDepartment of General Surgery Southern Medical University Hospital of Integrated Traditional Chinese and Western Medicine Southern Medical University Guangdong 510315 ChinaKey Laboratory of Applied Surface and Colloid Chemistry Ministry of Education School of Chemistry and Chemical Engineering Shaanxi Normal University Xi'an 710119 ChinaColorectal cancer (CRC) has a postoperative recurrence rate of up to 50%. Local treatment at the surgical site offers a new strategy to prevent recurrence, minimizing systemic side effects. However, these treatments often face issues like poor stability, insufficient targeting, and significant local side effects. Research shows glucose starvation can induce disulfidptosis, a novel cell death pathway, in CRC cells, suitable for precise intervention in residual tumor cells postsurgery. This study develops a biocompatible 2D therapeutic platform using amyloid‐like protein nanofilm technology for precise local synergistic intervention in disulfidptosis and photodynamic therapy (PDT) after CRC surgery. The nanofilm boasts excellent drug‐loading capacity, enzyme activity retention, ultrathin dimensions, wet adhesion, and biocompatibility. A model simulating incomplete tumor resection post‐CRC surgery shows that the GCI@nanofilm effectively inhibits recurrence through an enzyme‐catalyzed cascade reaction inducing disulfidptosis and synergizing with PDT. In summary, GCI@nanofilm overcomes multiple challenges in local postsurgical intervention, providing a foundation for precise and efficient treatment of residual tumors.https://doi.org/10.1002/smsc.2024003232D protein filmcolorectal cancer recurrencedisulfidptosissynergismwet interface adhesion
spellingShingle Man Zhang
Ke Li
Junhao Kou
Guozhi Lu
Ling Qiu
Chunzhao Yang
Yongchun Liu
Qi Xue
Peng Yang
Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
Small Science
2D protein film
colorectal cancer recurrence
disulfidptosis
synergism
wet interface adhesion
title Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
title_full Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
title_fullStr Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
title_full_unstemmed Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
title_short Functionalized Amyloid‐Like Protein Nanofilm‐Mediated Synergistic Disulfidptosis and Photodynamic Therapy for Preventing Postoperative Recurrence of Colorectal Cancer
title_sort functionalized amyloid like protein nanofilm mediated synergistic disulfidptosis and photodynamic therapy for preventing postoperative recurrence of colorectal cancer
topic 2D protein film
colorectal cancer recurrence
disulfidptosis
synergism
wet interface adhesion
url https://doi.org/10.1002/smsc.202400323
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