Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform

Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform

Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO2 laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is...

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Main Authors: Supawee Inlumphan, Winadda Wongwiriyapan, Narathon Khemasiri, Prapakorn Rattanawarinchai, Piyawan Leepheng, Panuwat Luengrojanakul, Tuksadon Wuttikhun, Michiko Obata, Masatsugu Fujishige, Kenji Takeuchi, Mayuree Phonyiem Reilly, Teerayut Uwanno, Mati Horprathum, Supanit Porntheeraphat, Kannika Sitthisuwannakul, Supranee Phanthanawiboon, Annop Klamchuen
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Sensors and Actuators Reports
Subjects:
Laser-induced graphene
Serology
Immunosensor
Electrochemical sensor
Dengue virus
Online Access:http://www.sciencedirect.com/science/article/pii/S2666053924000924
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Summary:Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO2 laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.
ISSN:2666-0539

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