Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor

Xanthan is microbial polysaccharide with outstanding rheological properties, non-toxic nature, biodegradability, and biocompatibility. This biopolymer is widely used in food, biomedical, pharmaceutical, petrochemical, chemical and textile industry. Industrial xanthan pr...

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Main Authors: Ida Zahović, Jelena Dodić, Damjan Vučurović, Bojana Bajić, Siniša Dodić, Zorana Trivunović
Format: Article
Language:English
Published: University of Szeged 2024-12-01
Series:Analecta
Online Access:https://www.analecta.hu/index.php/analecta/article/view/45836
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author Ida Zahović
Jelena Dodić
Damjan Vučurović
Bojana Bajić
Siniša Dodić
Zorana Trivunović
author_facet Ida Zahović
Jelena Dodić
Damjan Vučurović
Bojana Bajić
Siniša Dodić
Zorana Trivunović
author_sort Ida Zahović
collection DOAJ
description Xanthan is microbial polysaccharide with outstanding rheological properties, non-toxic nature, biodegradability, and biocompatibility. This biopolymer is widely used in food, biomedical, pharmaceutical, petrochemical, chemical and textile industry. Industrial xanthan production is generally conducted by aerobic submerged cultivation of Xanthomonas campestris strains on the media with glucose or sucrose under optimal conditions. Results from previous research indicate that xanthan can be successfully produced on media containing crude glycerol from biodiesel industry by different Xanthomonas species. The aim of this study was to examine the course of xanthan biosynthesis by the reference strain X. campestris ATCC 13951 in lab-scale bioreactor on medium containing crude glycerol generated in domestic biodiesel factory. The bioprocess was monitored by the analysis of cultivation medium samples taken in predetermined time intervals, and its success was estimated based on the xanthan concentration in the medium, separated biopolymer average molecular weight and degree of nutrients conversion. At the end of bioprocess, cultivation medium contained 12.34 g/L of xanthan with the average molecular weight of 3.04∙105 g/mol. Within this study, the achieved degree of glycerol, total nitrogen and total phosphorous conversion were 75.91%, 53.27% and 38.96%, respectively.
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publishDate 2024-12-01
publisher University of Szeged
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series Analecta
spelling doaj-art-31c09a618dcb441ca97f6f04d6afae2e2025-01-01T16:52:14ZengUniversity of SzegedAnalecta2064-79642024-12-01184213110.14232/analecta.2024.4.21-31Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale BioreactorIda Zahović0https://orcid.org/0000-0002-5735-5182Jelena Dodić1https://orcid.org/0000-0003-4858-1433Damjan Vučurović2https://orcid.org/0000-0002-0555-6089Bojana Bajić3https://orcid.org/0000-0001-9786-2376Siniša Dodić4https://orcid.org/0000-0002-3072-0549Zorana Trivunović5https://orcid.org/0000-0002-2889-219XUniversity of Novi SadUniversity of Novi SadUniversity of Novi SadUniversity of Novi SadUniversity of Novi SadUniversity of Novi Sad Xanthan is microbial polysaccharide with outstanding rheological properties, non-toxic nature, biodegradability, and biocompatibility. This biopolymer is widely used in food, biomedical, pharmaceutical, petrochemical, chemical and textile industry. Industrial xanthan production is generally conducted by aerobic submerged cultivation of Xanthomonas campestris strains on the media with glucose or sucrose under optimal conditions. Results from previous research indicate that xanthan can be successfully produced on media containing crude glycerol from biodiesel industry by different Xanthomonas species. The aim of this study was to examine the course of xanthan biosynthesis by the reference strain X. campestris ATCC 13951 in lab-scale bioreactor on medium containing crude glycerol generated in domestic biodiesel factory. The bioprocess was monitored by the analysis of cultivation medium samples taken in predetermined time intervals, and its success was estimated based on the xanthan concentration in the medium, separated biopolymer average molecular weight and degree of nutrients conversion. At the end of bioprocess, cultivation medium contained 12.34 g/L of xanthan with the average molecular weight of 3.04∙105 g/mol. Within this study, the achieved degree of glycerol, total nitrogen and total phosphorous conversion were 75.91%, 53.27% and 38.96%, respectively.https://www.analecta.hu/index.php/analecta/article/view/45836
spellingShingle Ida Zahović
Jelena Dodić
Damjan Vučurović
Bojana Bajić
Siniša Dodić
Zorana Trivunović
Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
Analecta
title Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
title_full Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
title_fullStr Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
title_full_unstemmed Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
title_short Examination of Xanthan Production on Biodiesel Industry Effluent-based Medium in Lab-scale Bioreactor
title_sort examination of xanthan production on biodiesel industry effluent based medium in lab scale bioreactor
url https://www.analecta.hu/index.php/analecta/article/view/45836
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