Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine
The aim of this study was to perform a comparison between quercetin and deferoxamine (DFO) on the heart, liver, and duodenum in iron overloaded rabbits. Four groups of adult rabbits (each of seven) used in the current study were treated as follows for 28 days: control (C): were injected intr...
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University of Baghdad, College of Veterinary Medicine
2023-12-01
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| Series: | The Iraqi Journal of Veterinary Medicine |
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| Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1444 |
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| author | Muntasser A Awad Baraa Al-Okaily |
| author_facet | Muntasser A Awad Baraa Al-Okaily |
| author_sort | Muntasser A Awad |
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The aim of this study was to perform a comparison between quercetin and deferoxamine (DFO) on the heart, liver, and duodenum in iron overloaded rabbits. Four groups of adult rabbits (each of seven) used in the current study were treated as follows for 28 days: control (C): were injected intraperitoneally (IP) normal saline every 72 hours + normal saline orally daily. T1, T2, and T3 groups were IP injected with iron dextran 100 mg/kg every 72 hours, T1 was left without treatment, group T2 was administrated with quercetin 350 mg/kg orally daily, and group T3 was injected with DFO 125 mg/kg intramuscularly (IM) daily. At the end of experiment, all animals were sacrificed, and tissue specimens were collected from the heart, liver, and duodenum for histopathological study. The results of T1 group showed edema, and iron deposition between the myocardial cells. The T2 group showed slight edema between the myocardium. T3 group showed edema, thickening, and congestion of blood vessels, all groups showed a positive result of the Prussian blue stain (PBS) compared to the control. The liver sections for the T1 group revealed iron overload, fibrosis, and hepatocyte necrosis. T2 and T3 groups showed mild vacuolation of hepatocytes, a decrease in iron overload, and these groups showed a positive result of the PBS compared to the control. Duodenum sections for T1 group showed hemochromatosis. while T2 group showed mild iron deposition. Positive result of PBS in T1 and T3 compared to the T2 and control were observed. Conclusion: Quercetin mitigates histological changes in iron overloaded rabbits due to its properties as an iron chelating agent compared to DFO.
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| format | Article |
| id | doaj-art-318aabd5acae4c5bafa69fd2e272ddb3 |
| institution | Kabale University |
| issn | 1609-5693 2410-7409 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | University of Baghdad, College of Veterinary Medicine |
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| series | The Iraqi Journal of Veterinary Medicine |
| spelling | doaj-art-318aabd5acae4c5bafa69fd2e272ddb32025-01-01T20:23:24ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092023-12-0147210.30539/5kcacw03Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with DeferoxamineMuntasser A Awad0https://orcid.org/0000-0001-9824-0755Baraa Al-Okaily1https://orcid.org/0000-0002-2691-2089Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqDepartment of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad The aim of this study was to perform a comparison between quercetin and deferoxamine (DFO) on the heart, liver, and duodenum in iron overloaded rabbits. Four groups of adult rabbits (each of seven) used in the current study were treated as follows for 28 days: control (C): were injected intraperitoneally (IP) normal saline every 72 hours + normal saline orally daily. T1, T2, and T3 groups were IP injected with iron dextran 100 mg/kg every 72 hours, T1 was left without treatment, group T2 was administrated with quercetin 350 mg/kg orally daily, and group T3 was injected with DFO 125 mg/kg intramuscularly (IM) daily. At the end of experiment, all animals were sacrificed, and tissue specimens were collected from the heart, liver, and duodenum for histopathological study. The results of T1 group showed edema, and iron deposition between the myocardial cells. The T2 group showed slight edema between the myocardium. T3 group showed edema, thickening, and congestion of blood vessels, all groups showed a positive result of the Prussian blue stain (PBS) compared to the control. The liver sections for the T1 group revealed iron overload, fibrosis, and hepatocyte necrosis. T2 and T3 groups showed mild vacuolation of hepatocytes, a decrease in iron overload, and these groups showed a positive result of the PBS compared to the control. Duodenum sections for T1 group showed hemochromatosis. while T2 group showed mild iron deposition. Positive result of PBS in T1 and T3 compared to the T2 and control were observed. Conclusion: Quercetin mitigates histological changes in iron overloaded rabbits due to its properties as an iron chelating agent compared to DFO. https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1444quercetindeferoxamineiron overloadheartliver |
| spellingShingle | Muntasser A Awad Baraa Al-Okaily Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine The Iraqi Journal of Veterinary Medicine quercetin deferoxamine iron overload heart liver |
| title | Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine |
| title_full | Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine |
| title_fullStr | Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine |
| title_full_unstemmed | Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine |
| title_short | Histopathological Changes of Heart, Liver, and Duodenum in Iron Overload: Comparing the Role of Quercetin with Deferoxamine |
| title_sort | histopathological changes of heart liver and duodenum in iron overload comparing the role of quercetin with deferoxamine |
| topic | quercetin deferoxamine iron overload heart liver |
| url | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1444 |
| work_keys_str_mv | AT muntasseraawad histopathologicalchangesofheartliverandduodenuminironoverloadcomparingtheroleofquercetinwithdeferoxamine AT baraaalokaily histopathologicalchangesofheartliverandduodenuminironoverloadcomparingtheroleofquercetinwithdeferoxamine |