Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis
Background Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model to provide a tool to investigate bacterial and viral pathogens in periodontitis.Methods The...
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2023.2292382 |
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| author | Anna Golda Anna Gasiorek Ewelina Dobosz Zuzanna Oruba Richard J. Lamont Jan Potempa Joanna Koziel |
| author_facet | Anna Golda Anna Gasiorek Ewelina Dobosz Zuzanna Oruba Richard J. Lamont Jan Potempa Joanna Koziel |
| author_sort | Anna Golda |
| collection | DOAJ |
| description | Background Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model to provide a tool to investigate bacterial and viral pathogens in periodontitis.Methods The OTG model composed of gingival fibroblasts (GFs) and telomerase-immortalized gingival keratinocytes (TIGKs) was constructed and applied to study infections by Porphyromonas gingivalis and herpes simplex virus 1 (HSV-1). Immunohistochemical staining, confocal microscopy, qPCR, titration techniques, and colony-forming unit counts were applied to interrogate epithelial markers expression, monitor P. gingivalis and HSV-1 presence, and evaluate the immune response along with the efficiency of antimicrobial drugs.Results The OTG model resembled the morphology of the human gingiva. During infection, both pathogens penetrated deep into the tissue and persisted for a few days with P. gingivalis also forming a biofilm on the cell surface. The infection triggered the expression of inflammatory mediators in cells and both pathogens were efficiently eliminated by specific antimicrobials.Conclusions Presented OTG model constitutes a simple and convenient tool to study the interaction between bacterial and viral pathogens within the gingival tissue, including penetration, persistence and biofilm formation. It is also suitable to examine the efficiency of antimicrobial drugs. |
| format | Article |
| id | doaj-art-3171bda982b540109ca9f533075f28a2 |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-3171bda982b540109ca9f533075f28a22024-11-26T06:52:11ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972024-12-0116110.1080/20002297.2023.2292382Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitisAnna Golda0Anna Gasiorek1Ewelina Dobosz2Zuzanna Oruba3Richard J. Lamont4Jan Potempa5Joanna Koziel6Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, PolandDepartment of Periodontology, Preventive Dentistry and Oral Pathology, Jagiellonian University Medical College, Faculty of Medicine, Krakow, PolandDepartment of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, University of Louisville, Louisville, KY, USADepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, PolandDepartment of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology of Jagiellonian University, Krakow, PolandBackground Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model to provide a tool to investigate bacterial and viral pathogens in periodontitis.Methods The OTG model composed of gingival fibroblasts (GFs) and telomerase-immortalized gingival keratinocytes (TIGKs) was constructed and applied to study infections by Porphyromonas gingivalis and herpes simplex virus 1 (HSV-1). Immunohistochemical staining, confocal microscopy, qPCR, titration techniques, and colony-forming unit counts were applied to interrogate epithelial markers expression, monitor P. gingivalis and HSV-1 presence, and evaluate the immune response along with the efficiency of antimicrobial drugs.Results The OTG model resembled the morphology of the human gingiva. During infection, both pathogens penetrated deep into the tissue and persisted for a few days with P. gingivalis also forming a biofilm on the cell surface. The infection triggered the expression of inflammatory mediators in cells and both pathogens were efficiently eliminated by specific antimicrobials.Conclusions Presented OTG model constitutes a simple and convenient tool to study the interaction between bacterial and viral pathogens within the gingival tissue, including penetration, persistence and biofilm formation. It is also suitable to examine the efficiency of antimicrobial drugs.https://www.tandfonline.com/doi/10.1080/20002297.2023.2292382Organotypicgingival modelPorphyromonas gingivalisherpes simplex virus-1infectiongingival tissue |
| spellingShingle | Anna Golda Anna Gasiorek Ewelina Dobosz Zuzanna Oruba Richard J. Lamont Jan Potempa Joanna Koziel Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis Journal of Oral Microbiology Organotypic gingival model Porphyromonas gingivalis herpes simplex virus-1 infection gingival tissue |
| title | Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| title_full | Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| title_fullStr | Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| title_full_unstemmed | Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| title_short | Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| title_sort | organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis |
| topic | Organotypic gingival model Porphyromonas gingivalis herpes simplex virus-1 infection gingival tissue |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2023.2292382 |
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