miR-92b-3p靶向肌细胞增强子2D抑制心肌细胞肥大

miR-92b-3p靶向肌细胞增强子2D抑制心肌细胞肥大

【目的】探讨微小RNA microRNA-92b-3p（miR-92b-3p）对心肌细胞肥大的调控作用及其作用靶基因。【方法】建 立血管紧张素Ⅱ（Ang-Ⅱ）诱导的C57BL/6小鼠心肌肥厚模型。通过尾静脉注射胆固醇修饰的miR-92b-3p模拟物（agomiR- 92b-3p）来增加小鼠心肌中miR-92b-3p水平，分别设立3个实验组：agomiR-negative control （agomiR-NC）+saline组，agomiR- NC+Ang-Ⅱ组，agomiR-92b-3p+Ang-Ⅱ组。用Ang-Ⅱ诱导乳小鼠心肌细胞建立心肌细胞肥大模型；双荧光素酶报告基因实验 检测mi...

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Bibliographic Details
Main Authors: 胡志琴, 朱杰宁, 林秋雄, 符永恒, 张梦珍, 吴书林, 单志新
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 2017-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:
心肌肥厚
microRNA-92b-3p
MEF2D
Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43567819/
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