Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae
Introduction: The emergence of multidrug-resistant pathogens, especially carbapenem-resistant Klebsiella pneumoniae (CRKP), is a pressing public health issue. The limited therapeutic choices further compound this. The escalating resistance to colistin, a crucial last-resort antibiotic, underscores...
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| Format: | Article |
| Language: | Spanish |
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ECIMED
2025-01-01
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| Series: | Revista Cubana de Medicina Militar |
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| Online Access: | https://revmedmilitar.sld.cu/index.php/mil/article/view/71523 |
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| author | Phu Thien Truong Ngoc Bich Tran Hoang Huy Pham Van Thi Hue Tran Hoang Thanh Phan Binh Thai Pham Lam Thi Ngoc Tran Truc Thi Thanh Nguyen Mai Le Phuong Dat Quoc Ngo |
| author_facet | Phu Thien Truong Ngoc Bich Tran Hoang Huy Pham Van Thi Hue Tran Hoang Thanh Phan Binh Thai Pham Lam Thi Ngoc Tran Truc Thi Thanh Nguyen Mai Le Phuong Dat Quoc Ngo |
| author_sort | Phu Thien Truong |
| collection | DOAJ |
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Introduction: The emergence of multidrug-resistant pathogens, especially carbapenem-resistant Klebsiella pneumoniae (CRKP), is a pressing public health issue. The limited therapeutic choices further compound this. The escalating resistance to colistin, a crucial last-resort antibiotic, underscores the need for precise and dependable antimicrobial susceptibility testing (AST) methods.
Objectives: To evaluate the performance of the Colistin Broth Disk Elution (CBDE) method in comparison to the reference Broth Microdilution (rBMD) method for detecting colistin resistance in CRKP isolates.
Methods: A cross-sectional study involving 129 non-duplicate CRKP isolates was conducted at Cho Ray Hospital from October 2023 to December 2023. Colistin susceptibility was assessed using both the BMD and CBDE methods. The agreement between the two methods was measured in terms of categorical agreement (CA) and essential agreement (EA) based on Clinical and Laboratory Standards Institute (CLSI) criteria.
Results: Of the 129 CRKP isolates, 23.26 % were identified as colistin-resistant by both BMD and CBDE methods. The CA between the two methods was 100 %, and the EA was 91.47 %, with no major errors (ME) or very major errors (VME) detected.
Conclusion: The CBDE method has shown a high level of agreement with the reference BMD method, making it a reliable alternative for the routine detection of colistin resistance in microbiology laboratories. The study's findings strongly support using CBDE as a practical and effective AST method for colistin, thereby contributing to antibiotic stewardship.
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| format | Article |
| id | doaj-art-304a8a8d3c094bedaed7c1a05eb71f97 |
| institution | Kabale University |
| issn | 1561-3046 |
| language | Spanish |
| publishDate | 2025-01-01 |
| publisher | ECIMED |
| record_format | Article |
| series | Revista Cubana de Medicina Militar |
| spelling | doaj-art-304a8a8d3c094bedaed7c1a05eb71f972025-01-14T19:00:02ZspaECIMEDRevista Cubana de Medicina Militar1561-30462025-01-01541Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniaePhu Thien Truong0https://orcid.org/0000-0003-0868-2811Ngoc Bich Tran1https://orcid.org/0000-0002-2255-6398Hoang Huy Pham2https://orcid.org/0009-0008-2090-5268Van Thi Hue Tran3https://orcid.org/0009-0007-0677-2553Hoang Thanh Phan4https://orcid.org/0009-0003-6737-7216Binh Thai Pham5https://orcid.org/0000-0002-2475-8006Lam Thi Ngoc Tran6https://orcid.org/0009-0005-9125-4771Truc Thi Thanh Nguyen7https://orcid.org/0009-0008-6117-4270Mai Le Phuong8https://orcid.org/0009-0001-9625-2522Dat Quoc Ngo9https://orcid.org/0000-0003-1461-0216University of Medicine and Pharmacy at Ho Chi Minh City. Ho Chi Minh City. Cho Ray Hospital. Ho Chi Minh City.university of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh cityuniversity of Medicine and Pharmacy at Ho Chi Minh city Introduction: The emergence of multidrug-resistant pathogens, especially carbapenem-resistant Klebsiella pneumoniae (CRKP), is a pressing public health issue. The limited therapeutic choices further compound this. The escalating resistance to colistin, a crucial last-resort antibiotic, underscores the need for precise and dependable antimicrobial susceptibility testing (AST) methods. Objectives: To evaluate the performance of the Colistin Broth Disk Elution (CBDE) method in comparison to the reference Broth Microdilution (rBMD) method for detecting colistin resistance in CRKP isolates. Methods: A cross-sectional study involving 129 non-duplicate CRKP isolates was conducted at Cho Ray Hospital from October 2023 to December 2023. Colistin susceptibility was assessed using both the BMD and CBDE methods. The agreement between the two methods was measured in terms of categorical agreement (CA) and essential agreement (EA) based on Clinical and Laboratory Standards Institute (CLSI) criteria. Results: Of the 129 CRKP isolates, 23.26 % were identified as colistin-resistant by both BMD and CBDE methods. The CA between the two methods was 100 %, and the EA was 91.47 %, with no major errors (ME) or very major errors (VME) detected. Conclusion: The CBDE method has shown a high level of agreement with the reference BMD method, making it a reliable alternative for the routine detection of colistin resistance in microbiology laboratories. The study's findings strongly support using CBDE as a practical and effective AST method for colistin, thereby contributing to antibiotic stewardship. https://revmedmilitar.sld.cu/index.php/mil/article/view/71523antimicrobial susceptibility testingcarbapenem resistancecolistinKlebsiella pneumoniaeMicrodilution |
| spellingShingle | Phu Thien Truong Ngoc Bich Tran Hoang Huy Pham Van Thi Hue Tran Hoang Thanh Phan Binh Thai Pham Lam Thi Ngoc Tran Truc Thi Thanh Nguyen Mai Le Phuong Dat Quoc Ngo Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae Revista Cubana de Medicina Militar antimicrobial susceptibility testing carbapenem resistance colistin Klebsiella pneumoniae Microdilution |
| title | Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae |
| title_full | Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae |
| title_fullStr | Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae |
| title_full_unstemmed | Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae |
| title_short | Colistin broth disk elution for the detection of Colistin resistance among Carbapenem-resistant Klebsiella pneumoniae |
| title_sort | colistin broth disk elution for the detection of colistin resistance among carbapenem resistant klebsiella pneumoniae |
| topic | antimicrobial susceptibility testing carbapenem resistance colistin Klebsiella pneumoniae Microdilution |
| url | https://revmedmilitar.sld.cu/index.php/mil/article/view/71523 |
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