Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone
Abstract Coconut oil is eatable oil with many nutritional and cosmetic applications. In this investigation coconut oil was subjected to 0 to 5 L/min of ozone for 3 h and the chemical composition of both crude and ozonized oil was valued via Gas Chromatography-Mass Spectrometry (GC–MS). Some biologic...
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| Format: | Article |
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SpringerOpen
2025-01-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-024-01813-1 |
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| author | Mohammed S. Almuhayawi Mohammed H. Alruhaili Muyassar K. Tarabulsi Soad K. Al Jaouni Abdulmajeed A. Alqurashi Faisal A. Alraddadi Duaa A. Bukhari Hibah M. Albasri Moayad S. Waznah Samy Selim |
| author_facet | Mohammed S. Almuhayawi Mohammed H. Alruhaili Muyassar K. Tarabulsi Soad K. Al Jaouni Abdulmajeed A. Alqurashi Faisal A. Alraddadi Duaa A. Bukhari Hibah M. Albasri Moayad S. Waznah Samy Selim |
| author_sort | Mohammed S. Almuhayawi |
| collection | DOAJ |
| description | Abstract Coconut oil is eatable oil with many nutritional and cosmetic applications. In this investigation coconut oil was subjected to 0 to 5 L/min of ozone for 3 h and the chemical composition of both crude and ozonized oil was valued via Gas Chromatography-Mass Spectrometry (GC–MS). Some biological tests were done including antibacterial action versus Helicobacter pylori, anti-biofilm activity versus H. pylori, anti-hemolytic activity in the existence of H. pylori, anti-Alzheimer action, and cytotoxic effect towards A-413 cancer cell line to determine the activity of coconut oil and upon exposure to ozone. Fifteen compounds were detected in the coconut oil crude and ozonized oils where the fatty acid esters were the most common molecules in crude coconut oil, whereas alkenes were the most predominant compounds in ozonized coconut oil. A slight elevation of antibacterial action towards H. pylori from 23.0 ± 0.1 to 28.2 ± 0.5 mm was displayed upon exposure of the coconut oil to ozone. Both crude and ozonized coconut oil showed a bactericidal effect with MICs = 62.5 ± 0.1, 125.0 ± 0.2 µg/mL and MBCs = 15.62 ± 0.2, 31.25 0.2 µg/mL for crude and ozonized oil, respectively. A significant elevation in anti-biofilm activity was found upon using 25% of MBCs of ozonized oil relative to crude oil. A dramatic rise was observed in anti-hemolytic activity upon using 25 and 75% of MICs of ozonized oil relative to crude one. A notable elevation of anti-Alzheimer impact was evident upon exposing coconut oil to ozone. Besides, the cytotoxic impact towards A-431 cells was slightly increased after exposing the oil to ozone. The current results suggest a new technique to expose coconut oil to ozone to improve some of its in vitro pharmaceutical applications. |
| format | Article |
| id | doaj-art-2ffb2b7459934dae94d69d3af624fc32 |
| institution | Kabale University |
| issn | 2191-0855 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj-art-2ffb2b7459934dae94d69d3af624fc322025-01-05T12:47:31ZengSpringerOpenAMB Express2191-08552025-01-0115111210.1186/s13568-024-01813-1Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozoneMohammed S. Almuhayawi0Mohammed H. Alruhaili1Muyassar K. Tarabulsi2Soad K. Al Jaouni3Abdulmajeed A. Alqurashi4Faisal A. Alraddadi5Duaa A. Bukhari6Hibah M. Albasri7Moayad S. Waznah8Samy Selim9Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz UniversityDepartment of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz UniversityDepartment of Basic Medical Sciences, College of Medicine, University of JeddahDepartment of Hematology/Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz UniversityDepartment of Biology, College of Science, Taibah UniversityDepartment of Biology, College of Science, Taibah UniversityDepartment of Biology, College of Science, Taibah UniversityDepartment of Biology, College of Science, Taibah UniversityDepartment of Biology, College of Science, Taibah UniversityDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf UniversityAbstract Coconut oil is eatable oil with many nutritional and cosmetic applications. In this investigation coconut oil was subjected to 0 to 5 L/min of ozone for 3 h and the chemical composition of both crude and ozonized oil was valued via Gas Chromatography-Mass Spectrometry (GC–MS). Some biological tests were done including antibacterial action versus Helicobacter pylori, anti-biofilm activity versus H. pylori, anti-hemolytic activity in the existence of H. pylori, anti-Alzheimer action, and cytotoxic effect towards A-413 cancer cell line to determine the activity of coconut oil and upon exposure to ozone. Fifteen compounds were detected in the coconut oil crude and ozonized oils where the fatty acid esters were the most common molecules in crude coconut oil, whereas alkenes were the most predominant compounds in ozonized coconut oil. A slight elevation of antibacterial action towards H. pylori from 23.0 ± 0.1 to 28.2 ± 0.5 mm was displayed upon exposure of the coconut oil to ozone. Both crude and ozonized coconut oil showed a bactericidal effect with MICs = 62.5 ± 0.1, 125.0 ± 0.2 µg/mL and MBCs = 15.62 ± 0.2, 31.25 0.2 µg/mL for crude and ozonized oil, respectively. A significant elevation in anti-biofilm activity was found upon using 25% of MBCs of ozonized oil relative to crude oil. A dramatic rise was observed in anti-hemolytic activity upon using 25 and 75% of MICs of ozonized oil relative to crude one. A notable elevation of anti-Alzheimer impact was evident upon exposing coconut oil to ozone. Besides, the cytotoxic impact towards A-431 cells was slightly increased after exposing the oil to ozone. The current results suggest a new technique to expose coconut oil to ozone to improve some of its in vitro pharmaceutical applications.https://doi.org/10.1186/s13568-024-01813-1CoconutOzoneH. pyloriGas chromatographyAnti-hemolyticAnti-Alzheimer |
| spellingShingle | Mohammed S. Almuhayawi Mohammed H. Alruhaili Muyassar K. Tarabulsi Soad K. Al Jaouni Abdulmajeed A. Alqurashi Faisal A. Alraddadi Duaa A. Bukhari Hibah M. Albasri Moayad S. Waznah Samy Selim Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone AMB Express Coconut Ozone H. pylori Gas chromatography Anti-hemolytic Anti-Alzheimer |
| title | Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| title_full | Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| title_fullStr | Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| title_full_unstemmed | Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| title_short | Pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| title_sort | pharmacological activities and phytochemical evaluation of coconut crude oil and upon exposure to ozone |
| topic | Coconut Ozone H. pylori Gas chromatography Anti-hemolytic Anti-Alzheimer |
| url | https://doi.org/10.1186/s13568-024-01813-1 |
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