RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer
Abstract Ovarian cancer is a disease with increasing incidence worldwide, and there is an urgent need for chemotherapy and biological targeted therapy. Epithelial-mesenchymal transformation (EMT) is an important initiation stage for tumor cells to acquire the ability to invade and metastasize. A gro...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-023-01103-7 |
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| author | Qiao Ping Xu Kui Deng Zhen Zhang Hongkai Shang |
| author_facet | Qiao Ping Xu Kui Deng Zhen Zhang Hongkai Shang |
| author_sort | Qiao Ping Xu |
| collection | DOAJ |
| description | Abstract Ovarian cancer is a disease with increasing incidence worldwide, and there is an urgent need for chemotherapy and biological targeted therapy. Epithelial-mesenchymal transformation (EMT) is an important initiation stage for tumor cells to acquire the ability to invade and metastasize. A growing number of findings suggest that human Schlafen family member 5(SLFN5) plays a key role in malignancy. However, the role of SLFN5 in ovarian cancer cells has not been fully elucidated. Samples were collected from patients with ovarian cancer diagnosed in Hangzhou First People's Hospital, and the expression of SLFN5 was detected by fluorescence quantitative PCR. The relationship between SLFN5 expression and the progression and malignancy of ovarian cancer was analyzed by using the expression profile data from the Cancer Genome Atlas (TCGA) database. The mRNA expression levels of SLFN5 related upstream and downstream signaling pathways were studied by fluorescence quantitative PCR. Silencing SLFN5 was performed by siRNA transfection. The expression of SLFN5 and transfer-related proteins was examined by Western blot. Transwell and wound healing experiments investigated the migration and invasion ability of ovarian cancer cells. TCGA database analysis results showed that in the population with high SLFN5 expression, compared with the group with low SLFN5 expression, OS was worse (P = 0.011). SLFN5 silencing had a significant inhibitory effect on EMT and invasion movement of ovarian cancer cells. RT-PCR method was used to detect the mRNA changes of SLFN5 in ovarian cancer tissue and adjacent tissue. It was found that the expression of SLFN5 in ovarian cancer tissue was increased, with a significant difference (P < 0.05). Together, these results suggest that SLFN5 may play a synergistic role in tumorigenesis and development of ovarian cancer cells, providing a potential target for future drug development for the treatment of ovarian cancer. |
| format | Article |
| id | doaj-art-2fd6ec4dd7c347fa8add4056433b8340 |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-2fd6ec4dd7c347fa8add4056433b83402024-12-15T12:11:39ZengBMCJournal of Ovarian Research1757-22152023-02-0116111410.1186/s13048-023-01103-7RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancerQiao Ping Xu0Kui Deng1Zhen Zhang2Hongkai Shang3Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou, First People’s HospitalWestlake Institute for Advanced StudyDepartment of Oncology, Hangzhou Cancer HospitalDepartment of Gynecology, Affiliated Hangzhou First People’s Hospital, Zhejiang University School of MedicineAbstract Ovarian cancer is a disease with increasing incidence worldwide, and there is an urgent need for chemotherapy and biological targeted therapy. Epithelial-mesenchymal transformation (EMT) is an important initiation stage for tumor cells to acquire the ability to invade and metastasize. A growing number of findings suggest that human Schlafen family member 5(SLFN5) plays a key role in malignancy. However, the role of SLFN5 in ovarian cancer cells has not been fully elucidated. Samples were collected from patients with ovarian cancer diagnosed in Hangzhou First People's Hospital, and the expression of SLFN5 was detected by fluorescence quantitative PCR. The relationship between SLFN5 expression and the progression and malignancy of ovarian cancer was analyzed by using the expression profile data from the Cancer Genome Atlas (TCGA) database. The mRNA expression levels of SLFN5 related upstream and downstream signaling pathways were studied by fluorescence quantitative PCR. Silencing SLFN5 was performed by siRNA transfection. The expression of SLFN5 and transfer-related proteins was examined by Western blot. Transwell and wound healing experiments investigated the migration and invasion ability of ovarian cancer cells. TCGA database analysis results showed that in the population with high SLFN5 expression, compared with the group with low SLFN5 expression, OS was worse (P = 0.011). SLFN5 silencing had a significant inhibitory effect on EMT and invasion movement of ovarian cancer cells. RT-PCR method was used to detect the mRNA changes of SLFN5 in ovarian cancer tissue and adjacent tissue. It was found that the expression of SLFN5 in ovarian cancer tissue was increased, with a significant difference (P < 0.05). Together, these results suggest that SLFN5 may play a synergistic role in tumorigenesis and development of ovarian cancer cells, providing a potential target for future drug development for the treatment of ovarian cancer.https://doi.org/10.1186/s13048-023-01103-7Ovarian cancerSLFN5Epithelial-mesenchymal transitionInvasion |
| spellingShingle | Qiao Ping Xu Kui Deng Zhen Zhang Hongkai Shang RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer Journal of Ovarian Research Ovarian cancer SLFN5 Epithelial-mesenchymal transition Invasion |
| title | RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| title_full | RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| title_fullStr | RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| title_full_unstemmed | RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| title_short | RETRACTED ARTICLE: SLFN5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| title_sort | retracted article slfn5 promotes reversible epithelial and mesenchymal transformation in ovarian cancer |
| topic | Ovarian cancer SLFN5 Epithelial-mesenchymal transition Invasion |
| url | https://doi.org/10.1186/s13048-023-01103-7 |
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