Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i>
<i>Pseudomonas plecoglossicida</i> infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on the protein composition of exosomes derived from the surface m...
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MDPI AG
2024-11-01
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| author | Dong Yang Xiaowan Ma Shengping Zhong Jiasen Guo Dewei Cheng Xuyang Chen Teng Huang Lixing Huang Ying Qiao Theerakamol Pengsakul |
| author_facet | Dong Yang Xiaowan Ma Shengping Zhong Jiasen Guo Dewei Cheng Xuyang Chen Teng Huang Lixing Huang Ying Qiao Theerakamol Pengsakul |
| author_sort | Dong Yang |
| collection | DOAJ |
| description | <i>Pseudomonas plecoglossicida</i> infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on the protein composition of exosomes derived from the surface mucus of the hybrid grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂. Two hundred healthy fish were randomly separated into challenge and control groups. Fish from the challenge group received 10<sup>3</sup> CFU/g of the bacterial pathogen <i>P. plecoglossicida</i> via intraperitoneal injection, while sterile PBS was used as a negative control. After injection, the mucus was collected and the exosomes were extracted for proteomic analysis. The results of proteomic analysis revealed that <i>P. plecoglossicida</i> infection significantly increased the levels of innate immune proteins, including lysosomal and peroxisomal proteins, within the exosomes. Furthermore, the CAD protein was found to play a pivotal role in the protein interaction networks involved in the response to <i>P. plecoglossicida</i> infection. Intriguingly, we also observed a significant increase in the levels of metal-binding proteins within the exosomes, providing important evidence of nutritional immunity on the surfaces of the fish hosts. Notably, several proteins, such as plasma kallikrein, Annexin A5, eukaryotic translation initiation factor 3 subunit M, and S-methyl-5-thioadenosine phosphorylase, exhibited a remarkable increase in abundance in exosomes after infection. These proteins show promising potential as noninvasive biomarkers for the diagnosis of visceral white spot disease. The study contributes to the understanding of the host response to <i>P. plecoglossicida</i> infection and may aid policymakers in implementing appropriate intervention measures for effective risk management of this devastating disease. |
| format | Article |
| id | doaj-art-2f70b0e9bf364d988a8c97f9bb37538a |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-2f70b0e9bf364d988a8c97f9bb37538a2024-12-13T16:21:04ZengMDPI AGAnimals2076-26152024-11-011423340110.3390/ani14233401Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i>Dong Yang0Xiaowan Ma1Shengping Zhong2Jiasen Guo3Dewei Cheng4Xuyang Chen5Teng Huang6Lixing Huang7Ying Qiao8Theerakamol Pengsakul9Guangxi University, Nanning 530200, ChinaGuangxi University, Nanning 530200, ChinaInstitute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, ChinaGuangxi University, Nanning 530200, ChinaFisheries College, Jimei University, Xiamen 361000, ChinaGuangxi University, Nanning 530200, ChinaHealth and Environmental Research Center, Faculty of Environmental Management, Prince of Songkla University, Hat Yai 90110, Thailand<i>Pseudomonas plecoglossicida</i> infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on the protein composition of exosomes derived from the surface mucus of the hybrid grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂. Two hundred healthy fish were randomly separated into challenge and control groups. Fish from the challenge group received 10<sup>3</sup> CFU/g of the bacterial pathogen <i>P. plecoglossicida</i> via intraperitoneal injection, while sterile PBS was used as a negative control. After injection, the mucus was collected and the exosomes were extracted for proteomic analysis. The results of proteomic analysis revealed that <i>P. plecoglossicida</i> infection significantly increased the levels of innate immune proteins, including lysosomal and peroxisomal proteins, within the exosomes. Furthermore, the CAD protein was found to play a pivotal role in the protein interaction networks involved in the response to <i>P. plecoglossicida</i> infection. Intriguingly, we also observed a significant increase in the levels of metal-binding proteins within the exosomes, providing important evidence of nutritional immunity on the surfaces of the fish hosts. Notably, several proteins, such as plasma kallikrein, Annexin A5, eukaryotic translation initiation factor 3 subunit M, and S-methyl-5-thioadenosine phosphorylase, exhibited a remarkable increase in abundance in exosomes after infection. These proteins show promising potential as noninvasive biomarkers for the diagnosis of visceral white spot disease. The study contributes to the understanding of the host response to <i>P. plecoglossicida</i> infection and may aid policymakers in implementing appropriate intervention measures for effective risk management of this devastating disease.https://www.mdpi.com/2076-2615/14/23/3401<i>Pseudomonas plecoglossicida</i>exosomesproteomic analysisbiomarker |
| spellingShingle | Dong Yang Xiaowan Ma Shengping Zhong Jiasen Guo Dewei Cheng Xuyang Chen Teng Huang Lixing Huang Ying Qiao Theerakamol Pengsakul Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> Animals <i>Pseudomonas plecoglossicida</i> exosomes proteomic analysis biomarker |
| title | Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> |
| title_full | Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> |
| title_fullStr | Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> |
| title_full_unstemmed | Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> |
| title_short | Mucosal Exosome Proteomics of Hybrid Grouper <i>Epinephelus fuscoguttatus</i>♀ × <i>E. lanceolatus</i>♂ Infected by <i>Pseudomonas plecoglossicida</i> |
| title_sort | mucosal exosome proteomics of hybrid grouper i epinephelus fuscoguttatus i ♀ i e lanceolatus i ♂ infected by i pseudomonas plecoglossicida i |
| topic | <i>Pseudomonas plecoglossicida</i> exosomes proteomic analysis biomarker |
| url | https://www.mdpi.com/2076-2615/14/23/3401 |
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