Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation
Abstract Sleep fragmentation (SF) is increasingly recognized as a contributing factor to postoperative cognitive dysfunction (POCD). Given the critical roles of somatostatin (SST) interneurons, associated gamma‐aminobutyric acid (GABA)ergic neurotransmitters, and hippocampal perfusion in sleep‐relat...
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Wiley
2024-12-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70153 |
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| author | Yun Li Jiafeng Yu Ningzhi Yang Siwen Long Yize Li Lina Zhao Yonghao Yu |
| author_facet | Yun Li Jiafeng Yu Ningzhi Yang Siwen Long Yize Li Lina Zhao Yonghao Yu |
| author_sort | Yun Li |
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| description | Abstract Sleep fragmentation (SF) is increasingly recognized as a contributing factor to postoperative cognitive dysfunction (POCD). Given the critical roles of somatostatin (SST) interneurons, associated gamma‐aminobutyric acid (GABA)ergic neurotransmitters, and hippocampal perfusion in sleep‐related cognition, this study examined changes in these mechanisms in preoperative SF affecting POCD induced by anesthesia/surgery in aged male mice. The Morris water maze (MWM), novel object recognition (NOR), and Y maze tests were utilized to evaluate POCD. Arterial spin labeling (ASL) was employed to measure hippocampal regional cerebral blood flow (rCBF). In vitro assays quantified the levels of GABAergic metabolites—such as SST, neuropeptide Y (NPY), glutamic acid decarboxylase 1 (GAD1), vesicular GABA transporter (VGAT), and GABA and the distribution of SST interneurons in the hippocampus through enzyme‐linked immunosorbent assay and immunofluorescence. Preoperative 24‐h SF exacerbated anesthesia/surgery‐induced spatial memory impairments observed in the MWM, NOR, and Y maze tests. Preoperative 24‐h SF significantly increased the number of SST interneurons in hippocampal CA1, elevated hippocampal levels of SST, NPY, GAD1, and GABA, and reduced the rCBF. Preoperative SF aggravated POCD in aged male mice, with an increased number of SST interneurons in hippocampal CA1, elevated hippocampal GABAergic metabolites, and a further reduction in rCBF. |
| format | Article |
| id | doaj-art-2f53936eed72460b9c20e6119cab8f9c |
| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
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| series | Physiological Reports |
| spelling | doaj-art-2f53936eed72460b9c20e6119cab8f9c2025-01-10T11:14:29ZengWileyPhysiological Reports2051-817X2024-12-011223n/an/a10.14814/phy2.70153Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentationYun Li0Jiafeng Yu1Ningzhi Yang2Siwen Long3Yize Li4Lina Zhao5Yonghao Yu6Department of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaDepartment of Critical Care Medicine Tianjin Medical University General Hospital Tianjin ChinaDepartment of Anesthesiology Tianjin Medical University General Hospital Tianjin ChinaAbstract Sleep fragmentation (SF) is increasingly recognized as a contributing factor to postoperative cognitive dysfunction (POCD). Given the critical roles of somatostatin (SST) interneurons, associated gamma‐aminobutyric acid (GABA)ergic neurotransmitters, and hippocampal perfusion in sleep‐related cognition, this study examined changes in these mechanisms in preoperative SF affecting POCD induced by anesthesia/surgery in aged male mice. The Morris water maze (MWM), novel object recognition (NOR), and Y maze tests were utilized to evaluate POCD. Arterial spin labeling (ASL) was employed to measure hippocampal regional cerebral blood flow (rCBF). In vitro assays quantified the levels of GABAergic metabolites—such as SST, neuropeptide Y (NPY), glutamic acid decarboxylase 1 (GAD1), vesicular GABA transporter (VGAT), and GABA and the distribution of SST interneurons in the hippocampus through enzyme‐linked immunosorbent assay and immunofluorescence. Preoperative 24‐h SF exacerbated anesthesia/surgery‐induced spatial memory impairments observed in the MWM, NOR, and Y maze tests. Preoperative 24‐h SF significantly increased the number of SST interneurons in hippocampal CA1, elevated hippocampal levels of SST, NPY, GAD1, and GABA, and reduced the rCBF. Preoperative SF aggravated POCD in aged male mice, with an increased number of SST interneurons in hippocampal CA1, elevated hippocampal GABAergic metabolites, and a further reduction in rCBF.https://doi.org/10.14814/phy2.70153aginghippocampusmagnetic resonance imagingPOCDsleep fragmentationsomatostatin |
| spellingShingle | Yun Li Jiafeng Yu Ningzhi Yang Siwen Long Yize Li Lina Zhao Yonghao Yu Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation Physiological Reports aging hippocampus magnetic resonance imaging POCD sleep fragmentation somatostatin |
| title | Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation |
| title_full | Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation |
| title_fullStr | Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation |
| title_full_unstemmed | Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation |
| title_short | Alterations in hippocampal somatostatin interneurons, GABAergic metabolism, and ASL perfusion in an aged male mouse model of POCD aggravated by sleep fragmentation |
| title_sort | alterations in hippocampal somatostatin interneurons gabaergic metabolism and asl perfusion in an aged male mouse model of pocd aggravated by sleep fragmentation |
| topic | aging hippocampus magnetic resonance imaging POCD sleep fragmentation somatostatin |
| url | https://doi.org/10.14814/phy2.70153 |
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