Determination of extended spectrum beta-lactamase producing escherichia coli strains in chicken meat samples
<b>Aim:</b> The aim of this study was to determine the prevalence of extended spectrum ?-lactamase (ESBL) producing E.coli in retailed chicken thigh, breast and wing meats, with and without packaging.<p> <b>Materials and Methods: </b>In the study, retailed 30 chicken th...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-10-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1296 |
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| Summary: | <b>Aim:</b> The aim of this study was to determine the prevalence of extended
spectrum ?-lactamase (ESBL) producing E.coli in retailed
chicken thigh, breast and wing meats, with and without packaging.<p>
<b>Materials and Methods: </b>In the study, retailed 30 chicken thigh, 30
chicken breast and 30 chicken wing samples, were used as materials.
After pre-enrichment, each sample was plated to the Brillance ESBL
medium and incubated. Up to 5 suspected colonies were selected
and plated to the EMB medium. Then the isolates were identified as
E. coli by IMViC test. In order to determine the ESBL production of
the isolates phenotypically combined disc diffusion test was performed
according to the CSLI.<p>
<b>Results:</b> From the 159 suspicious isolates, 152 (95.5%) of them
identified as E.coli. According to combined disc diffusion test 28
(18.4%) of the isolates were ESBL positive. 17 (60%) ESBL producing
isolates were found in the breast meat samples while 9 (32%)
isolates from thigh and 2 (7%) isolates from wings were positive for
ESBL production. The distribution of ESBL producing E. coli isolates
in packaged and unpackaged samples were determined as 71% and
28%, respectively.<p>
<b>Conclusion:</b> The presence of ESBL producing E. coli in chicken meats
indicates that foods may also be an important source for the transmission
of this resistance to human. Although foods are regularly
examined in the context of pathogenic bacteria, bacteria producing
GSBL are not covered by this. For this reason, it is suggested to determine
the isolates genotypically about this resistance by analysis. |
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| ISSN: | 1309-6958 2146-1953 |