The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes
Abstract Background Universal stress proteins (USPs) are prevalent in various bacteria to cope with different adverse stresses, while their possible effects on secondary metabolisms of hosts are unclear. Tiancimycins (TNMs) are ten-membered endiynes possessing excellent potential for development of...
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BMC
2024-12-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-024-02613-9 |
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| author | Huiming Liu Zhiying Fan Nian Tong Jing Lin Yong Huang Yanwen Duan Xiangcheng Zhu |
| author_facet | Huiming Liu Zhiying Fan Nian Tong Jing Lin Yong Huang Yanwen Duan Xiangcheng Zhu |
| author_sort | Huiming Liu |
| collection | DOAJ |
| description | Abstract Background Universal stress proteins (USPs) are prevalent in various bacteria to cope with different adverse stresses, while their possible effects on secondary metabolisms of hosts are unclear. Tiancimycins (TNMs) are ten-membered endiynes possessing excellent potential for development of anticancer antibody-drug conjugates. During our efforts to improve TNMs titer, a high-producing strain Streptomyces sp. CB03234-S had been obtained and its possible high yield mechanism is being continuously explored to further enhance TNMs production. Results In this work, the whole-genome resequencing and analysis results revealed a notable 583 kb terminal deletion containing 8 highly expressed usp genes in the genome of CB03234-S. The individual complementation of lost USPs in CB03234-S all showed differential effects on secondary metabolism, especially TNMs production. Among them, the overexpression of USP3 increased TNMs titer from 12.8 ± 0.2 to 31.1 ± 2.3 mg/L, while the overexpression of USP8 significantly reduced TNMs titer to only 1.0 ± 0.1 mg/L, but activated the production of porphyrin-type compounds. Subsequent genetic manipulations on USP3/USP8 orthologs in Streptomyces. coelicolor A3(2) and Streptomyces sp. CB00271 also presented clear effects on the secondary metabolisms of hosts. Further sequence similarity network analysis and Streptomyces-based pan‑genomic analysis suggested that the USP3/USP8 orthologs are widely distributed across Streptomyces. Conclusion Our studies shed light on the potential effects of USPs on secondary metabolisms of streptomycetes for the first time, and USPs could become novel targets for exploring and exploiting natural products in streptomycetes. |
| format | Article |
| id | doaj-art-2edc528bbe9b44f8bb59759e7dafc53a |
| institution | Kabale University |
| issn | 1475-2859 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj-art-2edc528bbe9b44f8bb59759e7dafc53a2024-12-22T12:56:55ZengBMCMicrobial Cell Factories1475-28592024-12-0123111010.1186/s12934-024-02613-9The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetesHuiming Liu0Zhiying Fan1Nian Tong2Jing Lin3Yong Huang4Yanwen Duan5Xiangcheng Zhu6Xiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityXiangya International Academy of Translational Medicine, Central South UniversityAbstract Background Universal stress proteins (USPs) are prevalent in various bacteria to cope with different adverse stresses, while their possible effects on secondary metabolisms of hosts are unclear. Tiancimycins (TNMs) are ten-membered endiynes possessing excellent potential for development of anticancer antibody-drug conjugates. During our efforts to improve TNMs titer, a high-producing strain Streptomyces sp. CB03234-S had been obtained and its possible high yield mechanism is being continuously explored to further enhance TNMs production. Results In this work, the whole-genome resequencing and analysis results revealed a notable 583 kb terminal deletion containing 8 highly expressed usp genes in the genome of CB03234-S. The individual complementation of lost USPs in CB03234-S all showed differential effects on secondary metabolism, especially TNMs production. Among them, the overexpression of USP3 increased TNMs titer from 12.8 ± 0.2 to 31.1 ± 2.3 mg/L, while the overexpression of USP8 significantly reduced TNMs titer to only 1.0 ± 0.1 mg/L, but activated the production of porphyrin-type compounds. Subsequent genetic manipulations on USP3/USP8 orthologs in Streptomyces. coelicolor A3(2) and Streptomyces sp. CB00271 also presented clear effects on the secondary metabolisms of hosts. Further sequence similarity network analysis and Streptomyces-based pan‑genomic analysis suggested that the USP3/USP8 orthologs are widely distributed across Streptomyces. Conclusion Our studies shed light on the potential effects of USPs on secondary metabolisms of streptomycetes for the first time, and USPs could become novel targets for exploring and exploiting natural products in streptomycetes.https://doi.org/10.1186/s12934-024-02613-9Universal stress proteinsTiancimycinsSecondary metabolismsStreptomycetesNatural products |
| spellingShingle | Huiming Liu Zhiying Fan Nian Tong Jing Lin Yong Huang Yanwen Duan Xiangcheng Zhu The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes Microbial Cell Factories Universal stress proteins Tiancimycins Secondary metabolisms Streptomycetes Natural products |
| title | The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| title_full | The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| title_fullStr | The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| title_full_unstemmed | The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| title_short | The exploration of high production of tiancimycins in Streptomyces sp. CB03234-S revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| title_sort | exploration of high production of tiancimycins in streptomyces sp cb03234 s revealed potential influences of universal stress proteins on secondary metabolisms of streptomycetes |
| topic | Universal stress proteins Tiancimycins Secondary metabolisms Streptomycetes Natural products |
| url | https://doi.org/10.1186/s12934-024-02613-9 |
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