Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes
Alveolar bone healing is influenced by various local and systemic factors, including the local inflammatory response. This study aimed to evaluate the role of inflammatory responsiveness in alveolar bone healing using 8-week-old male and female mice (N = 5/time/group) strains selected for maximum (A...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/13/12/972 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105689929285632 |
|---|---|
| author | Priscila Maria Colavite Michelle de Campos Soriani Azevedo Carolina Fávaro Francisconi Angélica Cristina Fonseca André Petenucci Tabanez Jéssica Lima Melchiades Daniela Carignatto Passadori Andrea Borrego Marcelo De Franco Ana Paula Favaro Trombone Gustavo Pompermaier Garlet |
| author_facet | Priscila Maria Colavite Michelle de Campos Soriani Azevedo Carolina Fávaro Francisconi Angélica Cristina Fonseca André Petenucci Tabanez Jéssica Lima Melchiades Daniela Carignatto Passadori Andrea Borrego Marcelo De Franco Ana Paula Favaro Trombone Gustavo Pompermaier Garlet |
| author_sort | Priscila Maria Colavite |
| collection | DOAJ |
| description | Alveolar bone healing is influenced by various local and systemic factors, including the local inflammatory response. This study aimed to evaluate the role of inflammatory responsiveness in alveolar bone healing using 8-week-old male and female mice (N = 5/time/group) strains selected for maximum (AIRmax) or minimum (AIRmin) acute inflammatory response carrying distinct homozygous RR/SS <i>Slc11a1</i> genotypes, namely AIRminRR, AIRminSS, AIRmaxRR, and AIRmaxSS mice. After upper right incisor extraction, bone healing was analyzed at 0, 3, 7, and 14 days using micro-computed tomography, histomorphometry, birefringence, immunohistochemistry, and PCRArray analysis. AIRmaxSS and AIRminRR presented the highest and lowest inflammatory readouts, respectively, associated with lowest repair levels in both strains, while intermediate inflammatory phenotypes observed in AIRminSS and AIRmaxRR were associated with higher repair levels in such strains. The better healing outcomes are associated with intermediate inflammatory cell counts, a balanced expression of pro- and anti-inflammatory cytokines and chemokines, increased expression of growth and osteogenic factors and MSCs markers. Our results demonstrate that extreme high and low inflammatory responses are not ideal for a proper bone repair outcome, while an intermediate and transitory inflammation is associated with a proper alveolar bone healing outcome. |
| format | Article |
| id | doaj-art-2ed53b185ab341cfa3c5babc5e345014 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-2ed53b185ab341cfa3c5babc5e3450142024-12-27T14:11:53ZengMDPI AGBiology2079-77372024-11-01131297210.3390/biology13120972Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory PhenotypesPriscila Maria Colavite0Michelle de Campos Soriani Azevedo1Carolina Fávaro Francisconi2Angélica Cristina Fonseca3André Petenucci Tabanez4Jéssica Lima Melchiades5Daniela Carignatto Passadori6Andrea Borrego7Marcelo De Franco8Ana Paula Favaro Trombone9Gustavo Pompermaier Garlet10Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilLaboratory of Immunogenetics, Butantan Institute, Secretary of Health, Government of the State of São Paulo, Sao Paulo CEP 05503-900, SP, BrazilLaboratory of Immunogenetics, Butantan Institute, Secretary of Health, Government of the State of São Paulo, Sao Paulo CEP 05503-900, SP, BrazilDepartment of Health Science, Universidade do Sagrado Coração, Bauru CEP 17011-160, SP, BrazilDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisola, 9-75, Bauru CEP 17012-901, SP, BrazilAlveolar bone healing is influenced by various local and systemic factors, including the local inflammatory response. This study aimed to evaluate the role of inflammatory responsiveness in alveolar bone healing using 8-week-old male and female mice (N = 5/time/group) strains selected for maximum (AIRmax) or minimum (AIRmin) acute inflammatory response carrying distinct homozygous RR/SS <i>Slc11a1</i> genotypes, namely AIRminRR, AIRminSS, AIRmaxRR, and AIRmaxSS mice. After upper right incisor extraction, bone healing was analyzed at 0, 3, 7, and 14 days using micro-computed tomography, histomorphometry, birefringence, immunohistochemistry, and PCRArray analysis. AIRmaxSS and AIRminRR presented the highest and lowest inflammatory readouts, respectively, associated with lowest repair levels in both strains, while intermediate inflammatory phenotypes observed in AIRminSS and AIRmaxRR were associated with higher repair levels in such strains. The better healing outcomes are associated with intermediate inflammatory cell counts, a balanced expression of pro- and anti-inflammatory cytokines and chemokines, increased expression of growth and osteogenic factors and MSCs markers. Our results demonstrate that extreme high and low inflammatory responses are not ideal for a proper bone repair outcome, while an intermediate and transitory inflammation is associated with a proper alveolar bone healing outcome.https://www.mdpi.com/2079-7737/13/12/972bone repairinflammation<i>Slc11a1</i> |
| spellingShingle | Priscila Maria Colavite Michelle de Campos Soriani Azevedo Carolina Fávaro Francisconi Angélica Cristina Fonseca André Petenucci Tabanez Jéssica Lima Melchiades Daniela Carignatto Passadori Andrea Borrego Marcelo De Franco Ana Paula Favaro Trombone Gustavo Pompermaier Garlet Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes Biology bone repair inflammation <i>Slc11a1</i> |
| title | Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes |
| title_full | Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes |
| title_fullStr | Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes |
| title_full_unstemmed | Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes |
| title_short | Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes |
| title_sort | intermediate and transitory inflammation mediate proper alveolar bone healing outcome in contrast to extreme low high responses evidence from mice strains selected for distinct inflammatory phenotypes |
| topic | bone repair inflammation <i>Slc11a1</i> |
| url | https://www.mdpi.com/2079-7737/13/12/972 |
| work_keys_str_mv | AT priscilamariacolavite intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT michelledecampossorianiazevedo intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT carolinafavarofrancisconi intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT angelicacristinafonseca intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT andrepetenuccitabanez intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT jessicalimamelchiades intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT danielacarignattopassadori intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT andreaborrego intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT marcelodefranco intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT anapaulafavarotrombone intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes AT gustavopompermaiergarlet intermediateandtransitoryinflammationmediateproperalveolarbonehealingoutcomeincontrasttoextremelowhighresponsesevidencefrommicestrainsselectedfordistinctinflammatoryphenotypes |