Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi
Abstract Plants are the rich source of biologically active compounds which can be obliging against various pathogenic microorganisms and cancerous diseases. The current study evaluated the antibacterial potential of aqueous, methanol, ethanol, and acetone extracts of Malus domestica (apple), Cinnamo...
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Nature Portfolio
2025-01-01
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| author | Asma Irshad Rabbia Jawad Qudsia Mushtaq Alexis Spalletta Patrick Martin Uzair Ishtiaq |
| author_facet | Asma Irshad Rabbia Jawad Qudsia Mushtaq Alexis Spalletta Patrick Martin Uzair Ishtiaq |
| author_sort | Asma Irshad |
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| description | Abstract Plants are the rich source of biologically active compounds which can be obliging against various pathogenic microorganisms and cancerous diseases. The current study evaluated the antibacterial potential of aqueous, methanol, ethanol, and acetone extracts of Malus domestica (apple), Cinnamomum verum (cinnamon) and Trachyspermum ammi (ajwain) via agar well diffusion methods and minimum inhibitory concentration (MIC) in (mm) against Staphylococcus aureus (ATCC 25923) and Salmonella typhi (ATCC 19430). The antioxidant properties including total phenolic content (TPC), total flavonoid content (TFC), DPPH and reducing power was determined by UV/VIS spectrophotometery and all the results interpreted through one way ANOVA (STATISTICA). In the results, methanolic and acetonic extracts of C. verum has shown maximum zone of inhibition (22.3 ± 0.58 mm) against S. aureus while for C. verum and T. ammi, ethanolic extracts has expressed the maximum zone of inhibition (22.3 ± 0.58 mm) against S. typhi and for M. domestica the methanolic extracts has exhibited highest zone of inhibition (18 ± 0.56 mm) among all other extracts of M. domestica. The MIC values were comparable with antimicrobial activity. Among the antioxidant activity analysis, the highest level of TPC has observed in aqueous extract of M. domestica 72.15 ± 1.80 mg GAE/g, while highest TFC was observed in methanolic extracts of M. domestica 15.62 ± 0.25 µg CE/g. The DPPH assay showed maximum percentage inhibition 123% in the methanolic extract of M. domestica, while highest reducing potential 13.42 ± 1.15 nm was observed in aqueous extract of C. verum. This study has compared three potential medicinal plants with biological active and eco-friendly components which play crucial role in therapeutics. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-2e48f7ce3cbb44e38efc4e0b88f99a962025-01-12T12:15:53ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-83506-0Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammiAsma Irshad0Rabbia Jawad1Qudsia Mushtaq2Alexis Spalletta3Patrick Martin4Uzair Ishtiaq5School of Biochemistry and Biotechnology, University of the PunjabSchool of Biological Sciences, University of the PunjabMicrobial Biotechnology Laboratory, Institute of zoology, University of the PunjabUnité Transformations & Agroressources, ULR7519, Univ. Artois - UnilasalleUnité Transformations & Agroressources, ULR7519, Univ. Artois - UnilasalleDepartment of Research and DevelopmentAbstract Plants are the rich source of biologically active compounds which can be obliging against various pathogenic microorganisms and cancerous diseases. The current study evaluated the antibacterial potential of aqueous, methanol, ethanol, and acetone extracts of Malus domestica (apple), Cinnamomum verum (cinnamon) and Trachyspermum ammi (ajwain) via agar well diffusion methods and minimum inhibitory concentration (MIC) in (mm) against Staphylococcus aureus (ATCC 25923) and Salmonella typhi (ATCC 19430). The antioxidant properties including total phenolic content (TPC), total flavonoid content (TFC), DPPH and reducing power was determined by UV/VIS spectrophotometery and all the results interpreted through one way ANOVA (STATISTICA). In the results, methanolic and acetonic extracts of C. verum has shown maximum zone of inhibition (22.3 ± 0.58 mm) against S. aureus while for C. verum and T. ammi, ethanolic extracts has expressed the maximum zone of inhibition (22.3 ± 0.58 mm) against S. typhi and for M. domestica the methanolic extracts has exhibited highest zone of inhibition (18 ± 0.56 mm) among all other extracts of M. domestica. The MIC values were comparable with antimicrobial activity. Among the antioxidant activity analysis, the highest level of TPC has observed in aqueous extract of M. domestica 72.15 ± 1.80 mg GAE/g, while highest TFC was observed in methanolic extracts of M. domestica 15.62 ± 0.25 µg CE/g. The DPPH assay showed maximum percentage inhibition 123% in the methanolic extract of M. domestica, while highest reducing potential 13.42 ± 1.15 nm was observed in aqueous extract of C. verum. This study has compared three potential medicinal plants with biological active and eco-friendly components which play crucial role in therapeutics.https://doi.org/10.1038/s41598-024-83506-0Plant extractsAntimicrobialAntioxidant |
| spellingShingle | Asma Irshad Rabbia Jawad Qudsia Mushtaq Alexis Spalletta Patrick Martin Uzair Ishtiaq Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi Scientific Reports Plant extracts Antimicrobial Antioxidant |
| title | Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi |
| title_full | Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi |
| title_fullStr | Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi |
| title_full_unstemmed | Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi |
| title_short | Determination of antibacterial and antioxidant potential of organic crude extracts from Malus domestica, Cinnamomum verum and Trachyspermum ammi |
| title_sort | determination of antibacterial and antioxidant potential of organic crude extracts from malus domestica cinnamomum verum and trachyspermum ammi |
| topic | Plant extracts Antimicrobial Antioxidant |
| url | https://doi.org/10.1038/s41598-024-83506-0 |
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