The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes
Aim: The purpose of this study was to evaluate the impact of gender and peripheral blood parameters on the characteristics of Leucocyte-and Platelet-Rich Fibrin (L-PRF) membranes and to describe histologically three different zones of L-PRF membranes. Methods: Blood was collected from twenty healthy...
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| Format: | Article |
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Elsevier
2022-11-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426822001117 |
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| author | Catherine Andrade Aldana Felipe Ugarte Amenabar Carolina Inostroza Silva Paulo Diaz Calderon David Rosenberg Messina Nelson Pinto Carrasco Marc Quirynen |
| author_facet | Catherine Andrade Aldana Felipe Ugarte Amenabar Carolina Inostroza Silva Paulo Diaz Calderon David Rosenberg Messina Nelson Pinto Carrasco Marc Quirynen |
| author_sort | Catherine Andrade Aldana |
| collection | DOAJ |
| description | Aim: The purpose of this study was to evaluate the impact of gender and peripheral blood parameters on the characteristics of Leucocyte-and Platelet-Rich Fibrin (L-PRF) membranes and to describe histologically three different zones of L-PRF membranes. Methods: Blood was collected from twenty healthy donors (10 men and 10 women). Peripheral blood parameters including leucocyte and platelet counts, and fibrinogen levels were recorded. L-PRF membranes were prepared to quantify the release of growth factors (PDGF, VEGF, BMP-2, and BMP-9) at 1, 2, 3 and 7 days and for histological examination. Three zones within each L-PRF membrane (face, body, and tail) were analysed separately, quantifying the area of leucocytes, platelets, and fibrin in percentage. The Young's modulus of the membranes was also considered (during tensile and compression tests). Results: Women had significantly higher fibrinogen levels in their peripheral blood, and a higher release of BMP-9, whereas men showed a significantly higher Young's modulus in compression tests. The histology revealed significant differences in cellular content and fibrin concentration between the 3 areas, with the face being biologically the richest. Conclusion: Several factors influenced the final characteristics of L-PRF membranes. These need to be taken into consideration when interpreting the results of research, but especially in clinical practice. |
| format | Article |
| id | doaj-art-2e3a992822af4864b329d1e9081ae38d |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-2e3a992822af4864b329d1e9081ae38d2024-11-23T06:29:36ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682022-11-01126753759The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranesCatherine Andrade Aldana0Felipe Ugarte Amenabar1Carolina Inostroza Silva2Paulo Diaz Calderon3David Rosenberg Messina4Nelson Pinto Carrasco5Marc Quirynen6Department of Periodontology and Oral Implantology, Faculty of Dentistry, Universidad de Los Andes, Santiago, Chile; Department of Oral Health Sciences, Periodontology, KU Leuven & Dentistry, University Hospitals, KU Leuven, Leuven, Belgium; Corresponding author. Monseñor Álvaro del Portillo 12.455, Las Condes, Santiago, Chile.Department of Periodontology and Oral Implantology, Faculty of Dentistry, Universidad de Los Andes, Santiago, ChileCenter of Biology and Oral Regeneration, Faculty of Dentistry, Universidad de Los Andes, Santiago, ChileBiopolymer Research and Engineering Laboratory (BIOPREL), School of Nutrition and Dietetics, Faculty of Medicine, Universidad de Los Andes, Santiago, ChileDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universidad de Los Andes, Santiago, ChileDepartment of Periodontology and Oral Implantology, Faculty of Dentistry, Universidad de Los Andes, Santiago, ChileDepartment of Oral Health Sciences, Periodontology, KU Leuven & Dentistry, University Hospitals, KU Leuven, Leuven, BelgiumAim: The purpose of this study was to evaluate the impact of gender and peripheral blood parameters on the characteristics of Leucocyte-and Platelet-Rich Fibrin (L-PRF) membranes and to describe histologically three different zones of L-PRF membranes. Methods: Blood was collected from twenty healthy donors (10 men and 10 women). Peripheral blood parameters including leucocyte and platelet counts, and fibrinogen levels were recorded. L-PRF membranes were prepared to quantify the release of growth factors (PDGF, VEGF, BMP-2, and BMP-9) at 1, 2, 3 and 7 days and for histological examination. Three zones within each L-PRF membrane (face, body, and tail) were analysed separately, quantifying the area of leucocytes, platelets, and fibrin in percentage. The Young's modulus of the membranes was also considered (during tensile and compression tests). Results: Women had significantly higher fibrinogen levels in their peripheral blood, and a higher release of BMP-9, whereas men showed a significantly higher Young's modulus in compression tests. The histology revealed significant differences in cellular content and fibrin concentration between the 3 areas, with the face being biologically the richest. Conclusion: Several factors influenced the final characteristics of L-PRF membranes. These need to be taken into consideration when interpreting the results of research, but especially in clinical practice.http://www.sciencedirect.com/science/article/pii/S2212426822001117FibrinogenGrowth factorsHistologyLeucocytesL-PRFPlatelet concentrates |
| spellingShingle | Catherine Andrade Aldana Felipe Ugarte Amenabar Carolina Inostroza Silva Paulo Diaz Calderon David Rosenberg Messina Nelson Pinto Carrasco Marc Quirynen The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes Journal of Oral Biology and Craniofacial Research Fibrinogen Growth factors Histology Leucocytes L-PRF Platelet concentrates |
| title | The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes |
| title_full | The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes |
| title_fullStr | The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes |
| title_full_unstemmed | The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes |
| title_short | The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes |
| title_sort | impact of gender and peripheral blood parameters on the characteristics of l prf membranes |
| topic | Fibrinogen Growth factors Histology Leucocytes L-PRF Platelet concentrates |
| url | http://www.sciencedirect.com/science/article/pii/S2212426822001117 |
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