Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics
Background: Saliva, an oral secretion is considered an essential biological modulator involved in maintaining oral homeostasis. Increased glucose levels in diabetic patients' saliva may have an impact on diversity of microbes. Comparing the salivary microflora of diabetic and non-diabetic cohor...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Journal of Oral Biology and Craniofacial Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426823001410 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846159447289757696 |
|---|---|
| author | Jyotsana Agarwal Pragya Pandey Shailendra K. Saxena Swatantra Kumar |
| author_facet | Jyotsana Agarwal Pragya Pandey Shailendra K. Saxena Swatantra Kumar |
| author_sort | Jyotsana Agarwal |
| collection | DOAJ |
| description | Background: Saliva, an oral secretion is considered an essential biological modulator involved in maintaining oral homeostasis. Increased glucose levels in diabetic patients' saliva may have an impact on diversity of microbes. Comparing the salivary microflora of diabetic and non-diabetic cohorts will help in diagnosis and risk assessment of oral health complications. This will provide greater knowledge about the contribution of oral microbes to the development of oral illnesses. The association between salivary microbiota and diabetic state is less explored in the North Indian population, hence current observational study was performed to analyze the salivary microflora of diabetic and non-diabetic individuals using metagenomic analysis. Materials and methods: This single-center non-randomized observational trial was conducted in Uttar Pradesh, India. Participants were enrolled into either diabetic (n = 68) or non-diabetic groups (n = 68) based on their diabetes status. Following saliva collection, DNA was extracted and metagenomic sequencing was performed. Results: Phylum Bacteroidetes and Fusobacteria were significantly abundant in diabetic individuals (p < 0.0001), while Proteobacteria was significantly higher among non-diabetic individuals (p < 0.0001). No statistical difference in phylum Actinobacteria and Firmicutes among diabetics and non-diabetics. Veillonella, Prevotella, Porphyromonas, Leptotrichia, Lactobacillus, and Streptococcus were greater in diabetics whereas the abundance of Capnocytophaga and Neisseria was more among non-diabetics (p < 0.05). Conclusions: The genera Veillonella, Prevotella, Porphyromonas, Leptotrichia, Lactobacillus, and Streptococcus were comparatively over the odds with the diabetics in India. The association between microbiota in diabetic population and risk related to increase in occurrence of caries, gingivitis, and periodontitis in diabetic population prevalence should be investigated. |
| format | Article |
| id | doaj-art-2e11cb69ec14430b9d1e017b25d6815c |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-2e11cb69ec14430b9d1e017b25d6815c2024-11-23T06:30:33ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-01-011412226Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomicsJyotsana Agarwal0Pragya Pandey1Shailendra K. Saxena2Swatantra Kumar3Department of Conservative Dentistry & Endodontics, King George's Medical University, Lucknow, IndiaDepartment of Conservative Dentistry & Endodontics, King George's Medical University, Lucknow, India; Corresponding author. Conservative Dentistry & Endodontics, King George's Medical University, Shahmina Shah road, Chowk, Lucknow, 226003, India.Centre for Advanced Research, King George's Medical University, Lucknow, IndiaCentre for Advanced Research, King George's Medical University, Lucknow, IndiaBackground: Saliva, an oral secretion is considered an essential biological modulator involved in maintaining oral homeostasis. Increased glucose levels in diabetic patients' saliva may have an impact on diversity of microbes. Comparing the salivary microflora of diabetic and non-diabetic cohorts will help in diagnosis and risk assessment of oral health complications. This will provide greater knowledge about the contribution of oral microbes to the development of oral illnesses. The association between salivary microbiota and diabetic state is less explored in the North Indian population, hence current observational study was performed to analyze the salivary microflora of diabetic and non-diabetic individuals using metagenomic analysis. Materials and methods: This single-center non-randomized observational trial was conducted in Uttar Pradesh, India. Participants were enrolled into either diabetic (n = 68) or non-diabetic groups (n = 68) based on their diabetes status. Following saliva collection, DNA was extracted and metagenomic sequencing was performed. Results: Phylum Bacteroidetes and Fusobacteria were significantly abundant in diabetic individuals (p < 0.0001), while Proteobacteria was significantly higher among non-diabetic individuals (p < 0.0001). No statistical difference in phylum Actinobacteria and Firmicutes among diabetics and non-diabetics. Veillonella, Prevotella, Porphyromonas, Leptotrichia, Lactobacillus, and Streptococcus were greater in diabetics whereas the abundance of Capnocytophaga and Neisseria was more among non-diabetics (p < 0.05). Conclusions: The genera Veillonella, Prevotella, Porphyromonas, Leptotrichia, Lactobacillus, and Streptococcus were comparatively over the odds with the diabetics in India. The association between microbiota in diabetic population and risk related to increase in occurrence of caries, gingivitis, and periodontitis in diabetic population prevalence should be investigated.http://www.sciencedirect.com/science/article/pii/S2212426823001410DiabeticsMetagenomicsMicrobiotaSalivaDental diseases |
| spellingShingle | Jyotsana Agarwal Pragya Pandey Shailendra K. Saxena Swatantra Kumar Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics Journal of Oral Biology and Craniofacial Research Diabetics Metagenomics Microbiota Saliva Dental diseases |
| title | Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics |
| title_full | Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics |
| title_fullStr | Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics |
| title_full_unstemmed | Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics |
| title_short | Comparative analysis of salivary microbiota in diabetic and non-diabetic individuals of North India using metagenomics |
| title_sort | comparative analysis of salivary microbiota in diabetic and non diabetic individuals of north india using metagenomics |
| topic | Diabetics Metagenomics Microbiota Saliva Dental diseases |
| url | http://www.sciencedirect.com/science/article/pii/S2212426823001410 |
| work_keys_str_mv | AT jyotsanaagarwal comparativeanalysisofsalivarymicrobiotaindiabeticandnondiabeticindividualsofnorthindiausingmetagenomics AT pragyapandey comparativeanalysisofsalivarymicrobiotaindiabeticandnondiabeticindividualsofnorthindiausingmetagenomics AT shailendraksaxena comparativeanalysisofsalivarymicrobiotaindiabeticandnondiabeticindividualsofnorthindiausingmetagenomics AT swatantrakumar comparativeanalysisofsalivarymicrobiotaindiabeticandnondiabeticindividualsofnorthindiausingmetagenomics |