Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis
Nuclear magnetic resonance (NMR) spectroscopy is gaining prominence as a vital quantitative method for sample analysis, with significant progress being made in the investigation of heteronuclei like phosphorus, a key element in numerous physiological functions. This paper provides a comprehensive re...
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2024-12-01
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| author | Yaqin Liu Lina Gao Zeling Yu |
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| description | Nuclear magnetic resonance (NMR) spectroscopy is gaining prominence as a vital quantitative method for sample analysis, with significant progress being made in the investigation of heteronuclei like phosphorus, a key element in numerous physiological functions. This paper provides a comprehensive review of the principles, methodologies, and applications of quantitative <sup>31</sup>P nuclear magnetic resonance (qNMR) spectroscopy. It begins with an introduction to the fundamental principles of NMR spectroscopy, highlighting the specific advantages of qNMR and the unique properties of the <sup>31</sup>P nucleus, including its high natural abundance and broad chemical shift range. While <sup>1</sup>H qNMR is widely used, signal overlap in complex mixtures can limit its accuracy. Additionally, this work explores diverse applications of <sup>31</sup>P qNMR across fields such as food analysis, pharmaceuticals, and biology, emphasizing its contributions to real-time drug quantification, metabolomics, and environmental analysis. A key advantage of <sup>31</sup>P NMR is its ability to provide exclusive detection and direct quantification of phosphorus in phosphorus-containing compounds. The internal standard method is favored for its simplicity, as it avoids the need for calibration curves, while the external standard method is better suited for natural products with established reference materials. This review aims to consolidate the applied prospects of <sup>31</sup>P qNMR, emphasizing its potential to expand the horizons of quantitative detection technologies and facilitate advancements in future research and practical applications. |
| format | Article |
| id | doaj-art-2e03f7587b9947eda16e1438dc471bfc |
| institution | Kabale University |
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| spelling | doaj-art-2e03f7587b9947eda16e1438dc471bfc2025-01-10T13:15:09ZengMDPI AGApplied Sciences2076-34172024-12-0115132310.3390/app15010323Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound AnalysisYaqin Liu0Lina Gao1Zeling Yu2Department of Chemistry, Zhejiang University, Hangzhou 310058, ChinaDepartment of Chemistry, Zhejiang University, Hangzhou 310058, ChinaDepartment of Chemistry, Zhejiang University, Hangzhou 310058, ChinaNuclear magnetic resonance (NMR) spectroscopy is gaining prominence as a vital quantitative method for sample analysis, with significant progress being made in the investigation of heteronuclei like phosphorus, a key element in numerous physiological functions. This paper provides a comprehensive review of the principles, methodologies, and applications of quantitative <sup>31</sup>P nuclear magnetic resonance (qNMR) spectroscopy. It begins with an introduction to the fundamental principles of NMR spectroscopy, highlighting the specific advantages of qNMR and the unique properties of the <sup>31</sup>P nucleus, including its high natural abundance and broad chemical shift range. While <sup>1</sup>H qNMR is widely used, signal overlap in complex mixtures can limit its accuracy. Additionally, this work explores diverse applications of <sup>31</sup>P qNMR across fields such as food analysis, pharmaceuticals, and biology, emphasizing its contributions to real-time drug quantification, metabolomics, and environmental analysis. A key advantage of <sup>31</sup>P NMR is its ability to provide exclusive detection and direct quantification of phosphorus in phosphorus-containing compounds. The internal standard method is favored for its simplicity, as it avoids the need for calibration curves, while the external standard method is better suited for natural products with established reference materials. This review aims to consolidate the applied prospects of <sup>31</sup>P qNMR, emphasizing its potential to expand the horizons of quantitative detection technologies and facilitate advancements in future research and practical applications.https://www.mdpi.com/2076-3417/15/1/323nuclear magnetic resonance spectroscopy (NMR)quantitative <sup>31</sup>P NMRphosphorus-containing compoundspharmaceutical analysisbiological applications |
| spellingShingle | Yaqin Liu Lina Gao Zeling Yu Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis Applied Sciences nuclear magnetic resonance spectroscopy (NMR) quantitative <sup>31</sup>P NMR phosphorus-containing compounds pharmaceutical analysis biological applications |
| title | Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis |
| title_full | Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis |
| title_fullStr | Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis |
| title_full_unstemmed | Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis |
| title_short | Quantitative <sup>31</sup>P NMR Spectroscopy: Principles, Methodologies, and Applications in Phosphorus-Containing Compound Analysis |
| title_sort | quantitative sup 31 sup p nmr spectroscopy principles methodologies and applications in phosphorus containing compound analysis |
| topic | nuclear magnetic resonance spectroscopy (NMR) quantitative <sup>31</sup>P NMR phosphorus-containing compounds pharmaceutical analysis biological applications |
| url | https://www.mdpi.com/2076-3417/15/1/323 |
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