Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury
Abstract Oxidative stress and inflammation are indispensable components of ischemia-reperfusion (IR) injury. In this study, we investigated the effects of low and high doses of caftaric acid (CA) on reducing kidney and remote organ damage induced by IR. We divided Wistar rats into four groups: sham,...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-82912-8 |
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| author | Fazile Nur Ekinci Akdemir Mustafa Can Güler Ersen Eraslan Ayhan Tanyeli Serkan Yildirim |
| author_facet | Fazile Nur Ekinci Akdemir Mustafa Can Güler Ersen Eraslan Ayhan Tanyeli Serkan Yildirim |
| author_sort | Fazile Nur Ekinci Akdemir |
| collection | DOAJ |
| description | Abstract Oxidative stress and inflammation are indispensable components of ischemia-reperfusion (IR) injury. In this study, we investigated the effects of low and high doses of caftaric acid (CA) on reducing kidney and remote organ damage induced by IR. We divided Wistar rats into four groups: sham, IR, low (40 mg/kg body weight (BW)), and high (80 mg/kg BW) CA groups. IR (1 h ischemia, 24 h reperfusion) was applied to all groups, except the sham one. Following the experimental period, we removed kidney and lung tissues to assess biochemical, histopathological, and immunohistochemical parameters. In the IR group, oxidant parameters (malondialdehyde (MDA), myeloperoxidase (MPO), total oxidant status (TOS), oxidative stress index (OSI)) increased, and antioxidant level parameters (superoxide dismutase (SOD) and total antioxidant status (TAS)) diminished. In addition, Microtubule-associated protein light chain 3 (LC3), cyclooxygenase-2 (COX-2), and caspase-3 immunopositivity were severe in the IR group. CA treatment improved the LC3, COX-2, and caspase-3 immunopositivity, lowered the oxidant level, and enhanced the antioxidant capacity. Histopathological findings were consistent with the data. In light of all our results, CA is effective against oxidative stress, autophagy, apoptosis, and inflammation in the renal IR experimental model. |
| format | Article |
| id | doaj-art-2e033be8ad584f73b6a7bbd2ee95b03b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-2e033be8ad584f73b6a7bbd2ee95b03b2024-12-29T12:19:01ZengNature PortfolioScientific Reports2045-23222024-12-0114111610.1038/s41598-024-82912-8Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injuryFazile Nur Ekinci Akdemir0Mustafa Can Güler1Ersen Eraslan2Ayhan Tanyeli3Serkan Yildirim4Department of Nutrition and Dietetics, Faculty of Health Science, Ağrı İbrahim Çeçen UniversityDepartment of Physiology, Faculty of Medicine, Atatürk UniversityDepartment of Physiology, Faculty of Medicine, Bandırma OnYedi Eylül UniversityDepartment of Physiology, Faculty of Medicine, Atatürk UniversityDepartment of Pathology, Faculty of Veterinary, Atatürk UniversityAbstract Oxidative stress and inflammation are indispensable components of ischemia-reperfusion (IR) injury. In this study, we investigated the effects of low and high doses of caftaric acid (CA) on reducing kidney and remote organ damage induced by IR. We divided Wistar rats into four groups: sham, IR, low (40 mg/kg body weight (BW)), and high (80 mg/kg BW) CA groups. IR (1 h ischemia, 24 h reperfusion) was applied to all groups, except the sham one. Following the experimental period, we removed kidney and lung tissues to assess biochemical, histopathological, and immunohistochemical parameters. In the IR group, oxidant parameters (malondialdehyde (MDA), myeloperoxidase (MPO), total oxidant status (TOS), oxidative stress index (OSI)) increased, and antioxidant level parameters (superoxide dismutase (SOD) and total antioxidant status (TAS)) diminished. In addition, Microtubule-associated protein light chain 3 (LC3), cyclooxygenase-2 (COX-2), and caspase-3 immunopositivity were severe in the IR group. CA treatment improved the LC3, COX-2, and caspase-3 immunopositivity, lowered the oxidant level, and enhanced the antioxidant capacity. Histopathological findings were consistent with the data. In light of all our results, CA is effective against oxidative stress, autophagy, apoptosis, and inflammation in the renal IR experimental model.https://doi.org/10.1038/s41598-024-82912-8Oxidative stressApoptosisAutophagyCaftaric acidIschemia-reperfusionAcute kidney injury |
| spellingShingle | Fazile Nur Ekinci Akdemir Mustafa Can Güler Ersen Eraslan Ayhan Tanyeli Serkan Yildirim Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury Scientific Reports Oxidative stress Apoptosis Autophagy Caftaric acid Ischemia-reperfusion Acute kidney injury |
| title | Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury |
| title_full | Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury |
| title_fullStr | Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury |
| title_full_unstemmed | Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury |
| title_short | Caftaric acid attenuates kidney and remote organ damage induced by renal ischemia-reperfusion injury |
| title_sort | caftaric acid attenuates kidney and remote organ damage induced by renal ischemia reperfusion injury |
| topic | Oxidative stress Apoptosis Autophagy Caftaric acid Ischemia-reperfusion Acute kidney injury |
| url | https://doi.org/10.1038/s41598-024-82912-8 |
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