Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice
This study investigated the effects of a xylitol-casein non-covalent complex (XC) on parameters related to type 2 diabetes mellitus (T2DM), in addition to related changes in gut microbiome composition and functions. High-fat-diet (HFD) + streptozotocin (STZ)-induced T2DM mice were treated with xylit...
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Tsinghua University Press
2024-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250221 |
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| author | Fanhua Kong Juan Zhang Shimo Kang Xinyu Shen Aicheng Liu Yan Zheng Junhua Shao Xiqing Yue |
| author_facet | Fanhua Kong Juan Zhang Shimo Kang Xinyu Shen Aicheng Liu Yan Zheng Junhua Shao Xiqing Yue |
| author_sort | Fanhua Kong |
| collection | DOAJ |
| description | This study investigated the effects of a xylitol-casein non-covalent complex (XC) on parameters related to type 2 diabetes mellitus (T2DM), in addition to related changes in gut microbiome composition and functions. High-fat-diet (HFD) + streptozotocin (STZ)-induced T2DM mice were treated with xylitol (XY), casein (CN), and XC, after which fecal samples were collected for gut microbiota composition and diversity analyses based on 16S rRNA high-throughput sequencing and multivariate statistics. XC decreased body weight and improved glucose tolerance, insulin sensitivity, pancreas impairment, blood lipid levels, and liver function in T2DM mice compared to XY- and CN-treated mice. Furthermore, XC modulated the α-diversity, β-diversity and gut microbiota composition. Based on Spearman’s correlation analysis, the relative abundances of Alistipes, Bacteroides, and Faecalibaculum were positively correlated and those of Akkermansia, Lactobacillus, Bifi dobacterium, and Turicibacter were negatively correlated with the phenotypes related to the improvement of T2DM. In conclusion, we found that XC alleviated insulin resistance by restoring the gut microbiota of T2DM mice. Our results provide strong evidence for the beneficial effects of XC on T2DM and motivation for further investigation in animal models and, eventually, human trials. |
| format | Article |
| id | doaj-art-2de9cf84eaed43a1a6ad3309e3f36f75 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2de9cf84eaed43a1a6ad3309e3f36f752025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011352741275310.26599/FSHW.2022.9250221Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus miceFanhua Kong0Juan Zhang1Shimo Kang2Xinyu Shen3Aicheng Liu4Yan Zheng5Junhua Shao6Xiqing Yue7College of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaCollege of Food Science, Shenyang Agriculture University, Shenyang 110866, ChinaThis study investigated the effects of a xylitol-casein non-covalent complex (XC) on parameters related to type 2 diabetes mellitus (T2DM), in addition to related changes in gut microbiome composition and functions. High-fat-diet (HFD) + streptozotocin (STZ)-induced T2DM mice were treated with xylitol (XY), casein (CN), and XC, after which fecal samples were collected for gut microbiota composition and diversity analyses based on 16S rRNA high-throughput sequencing and multivariate statistics. XC decreased body weight and improved glucose tolerance, insulin sensitivity, pancreas impairment, blood lipid levels, and liver function in T2DM mice compared to XY- and CN-treated mice. Furthermore, XC modulated the α-diversity, β-diversity and gut microbiota composition. Based on Spearman’s correlation analysis, the relative abundances of Alistipes, Bacteroides, and Faecalibaculum were positively correlated and those of Akkermansia, Lactobacillus, Bifi dobacterium, and Turicibacter were negatively correlated with the phenotypes related to the improvement of T2DM. In conclusion, we found that XC alleviated insulin resistance by restoring the gut microbiota of T2DM mice. Our results provide strong evidence for the beneficial effects of XC on T2DM and motivation for further investigation in animal models and, eventually, human trials.https://www.sciopen.com/article/10.26599/FSHW.2022.9250221xylitol-casein complextype 2 diabetes mellitusinsulin resistancegut microbiota |
| spellingShingle | Fanhua Kong Juan Zhang Shimo Kang Xinyu Shen Aicheng Liu Yan Zheng Junhua Shao Xiqing Yue Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice Food Science and Human Wellness xylitol-casein complex type 2 diabetes mellitus insulin resistance gut microbiota |
| title | Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice |
| title_full | Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice |
| title_fullStr | Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice |
| title_full_unstemmed | Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice |
| title_short | Effects of axylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice |
| title_sort | effects of axylitol casein complex on insulin resistance and gut microbiota composition in high fat diet streptozotocin induced type 2 diabetes mellitus mice |
| topic | xylitol-casein complex type 2 diabetes mellitus insulin resistance gut microbiota |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250221 |
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