Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia
Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study w...
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| Language: | English |
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International Journal of Secondary Metabolite
2024-08-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3471966 |
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| author | Rebecca Beyene Aman Dekebo Teshome Biru |
| author_facet | Rebecca Beyene Aman Dekebo Teshome Biru |
| author_sort | Rebecca Beyene |
| collection | DOAJ |
| description | Microbial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis (500 g) was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mg/mL to 200 mg/mL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.33±1.52 mm, Escherichia coli 14.33±1.53 mm and Salmonella typhimurium 16.00±1.00 mm, respectively. MeOH extract (1000 µg/mL) showed superior radical scavenging property (0.02) than ascorbic acid (0.05). The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4 % of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance. |
| format | Article |
| id | doaj-art-2ddea6aa04794a47b5f8771d52b38a2e |
| institution | Kabale University |
| issn | 2148-6905 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-2ddea6aa04794a47b5f8771d52b38a2e2024-12-06T11:03:54ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052024-08-0111349450610.21448/ijsm.1375324618Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from EthiopiaRebecca Beyene0https://orcid.org/0009-0001-6769-4014Aman Dekebo1https://orcid.org/0000-0003-4767-606XTeshome Biru2https://orcid.org/0000-0002-3858-7105Adama Science and Technology UniversityAdama Science and Technology UniversityAdama Science and Technology UniversityMicrobial resistance to antibiotics and the shortage of efficient antimicrobial agent has necessitated the search for a better antimicrobial agent from various sources. Plants secondary metabolites are the major sources for discovery of new bioactive chemical compounds. The objective of this study was to determine the antibacterial and antioxidant activities of Laurus nobilis leaf extract and its essential oil against human pathogenic microorganisms and to analyse its chemical composition. The leaf of L. nobilis (500 g) was air-dried, powdered and extracted using four different solvents. The crude extract and the essential oil were tested against four Gram-negative and two Gram-positive bacterial strains. The radical scavenging activity of the crud extract was examined using DPPH assay. Bacterial inhibition activity of the crude extract increased with increased concentration from 25 mg/mL to 200 mg/mL. The maximum inhibition zone was recorded against Enterococcus faecalis 13.33±1.52 mm, Escherichia coli 14.33±1.53 mm and Salmonella typhimurium 16.00±1.00 mm, respectively. MeOH extract (1000 µg/mL) showed superior radical scavenging property (0.02) than ascorbic acid (0.05). The analysis of the oil using GC-MS indicated the presence of 48 chemical substances accounting for 91.4 % of the total compositions. The finding of this study showed that bay leaf has considerable antimicrobial and antioxidant activities. Further evaluation of this plant is recommended with particular focus on the mechanisms of action of the antimicrobial substance.https://dergipark.org.tr/en/download/article-file/3471966antimicrobial antioxidantbay leafessential oilantimicrobial antioxidantbay leafessential oil |
| spellingShingle | Rebecca Beyene Aman Dekebo Teshome Biru Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia International Journal of Secondary Metabolite antimicrobial antioxidant bay leaf essential oil antimicrobial antioxidant bay leaf essential oil |
| title | Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia |
| title_full | Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia |
| title_fullStr | Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia |
| title_full_unstemmed | Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia |
| title_short | Antibacterial and antioxidant properties and phytochemical screening of Laurus nobilis L. extract from Ethiopia |
| title_sort | antibacterial and antioxidant properties and phytochemical screening of laurus nobilis l extract from ethiopia |
| topic | antimicrobial antioxidant bay leaf essential oil antimicrobial antioxidant bay leaf essential oil |
| url | https://dergipark.org.tr/en/download/article-file/3471966 |
| work_keys_str_mv | AT rebeccabeyene antibacterialandantioxidantpropertiesandphytochemicalscreeningoflaurusnobilislextractfromethiopia AT amandekebo antibacterialandantioxidantpropertiesandphytochemicalscreeningoflaurusnobilislextractfromethiopia AT teshomebiru antibacterialandantioxidantpropertiesandphytochemicalscreeningoflaurusnobilislextractfromethiopia |