Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT
Abstract This article focusing on examining the function and further, molecular function of SHP2 in ovarian cancer (OC). For the molecular mechanism, bioinformatics was applied to study the specifically expressed genes in ovarian cancer ; the western blotting was applied to identify the EGF, p-SHP2,...
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Nature Portfolio
2025-01-01
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| author | Xiaofei Li Haibo Zhang Jianan Dong Juan Wang |
| author_facet | Xiaofei Li Haibo Zhang Jianan Dong Juan Wang |
| author_sort | Xiaofei Li |
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| description | Abstract This article focusing on examining the function and further, molecular function of SHP2 in ovarian cancer (OC). For the molecular mechanism, bioinformatics was applied to study the specifically expressed genes in ovarian cancer ; the western blotting was applied to identify the EGF, p-SHP2, ZEB1, and E-Cadherin expressions in ovarian cancer tissue and pair adjacent tissue; then SKOV3 cells were treated with EGF and infected with E-Cadherin overexpression lentivirus, and then cells were treated with benzyl butyl phthalate and IRS-1 respectively. Detection of expression of p-SHP2, ZEB1, E-Cadherin, α3-integrin, p-Src, p-SMAD2, Snail, Slug and SKOV3 cells of migration and invasion abilities were detected using Western blot method and cell scratch assay and Transwell assay; Progression of ovarian cancer was detected using subcutaneous tumor transplantation assay in nude mice and HE staining method and immunocyto. The bioinformatics analysis results suggested that SHP2 is highly specifically expressed and E-Cadherin, which is low specifically expressed in ovarian cancer tissues, while EGF, p-SHP2 or ZEB1 are highly expressed and E-Cadherin is low expressed in ovarian cancer tissues; Overexpression of E-Cadherin could reduce the expressions of p-SHP2, ZEB1, α3-integrin, p-Src, p-SMAD2, Snail and Slug might has roles in alleviating the ovarian cancer development and decreasing the levels of p-SHP2 and ZEB1 in tumor samples. And E-Cadherin overexpression reduced the migration and invasion ability of SKOV3 cells. SHP2 tyrosine phosphatase enhances the ovarian cancer cells’ motility and invasiveness by upregulation of the integrin/E-Cadherin switch through ZEB1 signal. |
| format | Article |
| id | doaj-art-2dba70d6850743cb9370f39b62c3426d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-2dba70d6850743cb9370f39b62c3426d2025-01-12T12:21:48ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85445-wTyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMTXiaofei Li0Haibo Zhang1Jianan Dong2Juan Wang3Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical UniversityDepartment of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical UniversityDepartment of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical UniversityDepartment of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical UniversityAbstract This article focusing on examining the function and further, molecular function of SHP2 in ovarian cancer (OC). For the molecular mechanism, bioinformatics was applied to study the specifically expressed genes in ovarian cancer ; the western blotting was applied to identify the EGF, p-SHP2, ZEB1, and E-Cadherin expressions in ovarian cancer tissue and pair adjacent tissue; then SKOV3 cells were treated with EGF and infected with E-Cadherin overexpression lentivirus, and then cells were treated with benzyl butyl phthalate and IRS-1 respectively. Detection of expression of p-SHP2, ZEB1, E-Cadherin, α3-integrin, p-Src, p-SMAD2, Snail, Slug and SKOV3 cells of migration and invasion abilities were detected using Western blot method and cell scratch assay and Transwell assay; Progression of ovarian cancer was detected using subcutaneous tumor transplantation assay in nude mice and HE staining method and immunocyto. The bioinformatics analysis results suggested that SHP2 is highly specifically expressed and E-Cadherin, which is low specifically expressed in ovarian cancer tissues, while EGF, p-SHP2 or ZEB1 are highly expressed and E-Cadherin is low expressed in ovarian cancer tissues; Overexpression of E-Cadherin could reduce the expressions of p-SHP2, ZEB1, α3-integrin, p-Src, p-SMAD2, Snail and Slug might has roles in alleviating the ovarian cancer development and decreasing the levels of p-SHP2 and ZEB1 in tumor samples. And E-Cadherin overexpression reduced the migration and invasion ability of SKOV3 cells. SHP2 tyrosine phosphatase enhances the ovarian cancer cells’ motility and invasiveness by upregulation of the integrin/E-Cadherin switch through ZEB1 signal.https://doi.org/10.1038/s41598-025-85445-wOvarian cancerSHP2Eintegrin/E-Cadherin signalling pathwayEpithelial mesenchymal transition |
| spellingShingle | Xiaofei Li Haibo Zhang Jianan Dong Juan Wang Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT Scientific Reports Ovarian cancer SHP2 Eintegrin/E-Cadherin signalling pathway Epithelial mesenchymal transition |
| title | Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT |
| title_full | Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT |
| title_fullStr | Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT |
| title_full_unstemmed | Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT |
| title_short | Tyrosine phosphatase SHP2 accelerated ovarian cancer via modulating integrin/ E-Cadherin/ ZEB1 induced EMT |
| title_sort | tyrosine phosphatase shp2 accelerated ovarian cancer via modulating integrin e cadherin zeb1 induced emt |
| topic | Ovarian cancer SHP2 Eintegrin/E-Cadherin signalling pathway Epithelial mesenchymal transition |
| url | https://doi.org/10.1038/s41598-025-85445-w |
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