Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography
Avian influenza is a significant threat to the poultry industry, and it has become an outbreak in many countries because of its mortality and morbidity. Concerns about the history of avian influenza outbreaks has prompted all countries to enhance their independence in pharmaceutical and biological c...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000136 |
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| author | Armanda Dwi Prayugo Toto Subroto Wyanda Arnafia Wahyu Widayat Dinda Reisinta Akmal Jauhari Siti Munawaroh Denniswara Sibit Idar Idar |
| author_facet | Armanda Dwi Prayugo Toto Subroto Wyanda Arnafia Wahyu Widayat Dinda Reisinta Akmal Jauhari Siti Munawaroh Denniswara Sibit Idar Idar |
| author_sort | Armanda Dwi Prayugo |
| collection | DOAJ |
| description | Avian influenza is a significant threat to the poultry industry, and it has become an outbreak in many countries because of its mortality and morbidity. Concerns about the history of avian influenza outbreaks has prompted all countries to enhance their independence in pharmaceutical and biological components as a preparedness measure for any potential occurrences. The production of antibodies such as IgY is a potential alternative. The potential of IgY biomaterials can be utilized as bioreceptors in diagnostic tools. This research aimed to purify IgY anti-avian influenza H5N1 produced from chicken eggs. IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated at 0.5 ml per bird dose using inactivated H5N1 vaccine with a purification stage from graded PEG, hydrophobic interaction chromatography, and concentrated using an ultrafilter. The present study shows that the IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated with an inactivated H5N1 vaccine, from 4 chicken eggs with a total protein content of 40.01 mg, it has biological activity with HI titer activity of 4,915.2 HI units and a recovery value of 24 % with hydrophobic interaction chromatography. Therefore, the purification process with hydrophobic interaction chromatography does not remove the IgY biological antibody. Further development will be required to perform |
| format | Article |
| id | doaj-art-2db6714e489c438e9eecaa81ea7a047f |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-2db6714e489c438e9eecaa81ea7a047f2025-01-09T06:12:10ZengElsevierPoultry Science0032-57912025-02-011042104776Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatographyArmanda Dwi Prayugo0Toto Subroto1Wyanda Arnafia2Wahyu Widayat3Dinda Reisinta4Akmal Jauhari5Siti Munawaroh6Denniswara Sibit7Idar Idar8Department of Biomedical Science, Veterinary Medicine Program, Faculty of Medicine, Universitas Padjadjaran, Sumedang, West Java, 45363, Indonesia; Corresponding author.Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, West Java, 45363, Indonesia; Research Center for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, West Java, 40132, IndonesiaDepartment of Medical Microbiology, School of Veterinary Medicine, IPB University, Bogor, West Java, 16680, IndonesiaResearch Center for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, West Java, 40132, Indonesia; Faculty of Pharmacy, Universitas Mulawarman, Samarinda 75119, IndonesiaResearch and Development Division, PT Tekad Mandiri Citra, Bandung, West Java, 40292, IndonesiaResearch and Development Division, PT Tekad Mandiri Citra, Bandung, West Java, 40292, IndonesiaResearch and Development Division, PT Tekad Mandiri Citra, Bandung, West Java, 40292, IndonesiaResearch and Development Division, PT Tekad Mandiri Citra, Bandung, West Java, 40292, IndonesiaResearch Center for Molecular Biotechnology and Bioinformatics, Universitas Padjadjaran, West Java, 40132, Indonesia; Faculty of Pharmacy, Universitas Bhakti Kencana, West Java, 40614, IndonesiaAvian influenza is a significant threat to the poultry industry, and it has become an outbreak in many countries because of its mortality and morbidity. Concerns about the history of avian influenza outbreaks has prompted all countries to enhance their independence in pharmaceutical and biological components as a preparedness measure for any potential occurrences. The production of antibodies such as IgY is a potential alternative. The potential of IgY biomaterials can be utilized as bioreceptors in diagnostic tools. This research aimed to purify IgY anti-avian influenza H5N1 produced from chicken eggs. IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated at 0.5 ml per bird dose using inactivated H5N1 vaccine with a purification stage from graded PEG, hydrophobic interaction chromatography, and concentrated using an ultrafilter. The present study shows that the IgY anti-avian influenza H5N1 can be obtained from chickens vaccinated with an inactivated H5N1 vaccine, from 4 chicken eggs with a total protein content of 40.01 mg, it has biological activity with HI titer activity of 4,915.2 HI units and a recovery value of 24 % with hydrophobic interaction chromatography. Therefore, the purification process with hydrophobic interaction chromatography does not remove the IgY biological antibody. Further development will be required to performhttp://www.sciencedirect.com/science/article/pii/S0032579125000136Avian influenzaPoultryImmunoglobulin yolkHydrophobic interaction chromatographyPurification |
| spellingShingle | Armanda Dwi Prayugo Toto Subroto Wyanda Arnafia Wahyu Widayat Dinda Reisinta Akmal Jauhari Siti Munawaroh Denniswara Sibit Idar Idar Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography Poultry Science Avian influenza Poultry Immunoglobulin yolk Hydrophobic interaction chromatography Purification |
| title | Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography |
| title_full | Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography |
| title_fullStr | Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography |
| title_full_unstemmed | Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography |
| title_short | Purification immunoglobulin yolk anti avian influenza H5N1 in poultry using hydrophobic interaction chromatography |
| title_sort | purification immunoglobulin yolk anti avian influenza h5n1 in poultry using hydrophobic interaction chromatography |
| topic | Avian influenza Poultry Immunoglobulin yolk Hydrophobic interaction chromatography Purification |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000136 |
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