Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice
Dietary parboiled rice (PR) has a low risk of disease, but little is known about the contribution of PR to the prevention of hyperlipidemia. The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study. Male C57BL/6J mice were fed with a normal diet, high-fat die...
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Tsinghua University Press
2024-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250120 |
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| author | Xiuxiu Wu Tianyi Guo Biao Li Shuai Han Zuomin Hu Yi Luo Dandan Qin Yaping Zhou Feijun Luo Qinlu Lin |
| author_facet | Xiuxiu Wu Tianyi Guo Biao Li Shuai Han Zuomin Hu Yi Luo Dandan Qin Yaping Zhou Feijun Luo Qinlu Lin |
| author_sort | Xiuxiu Wu |
| collection | DOAJ |
| description | Dietary parboiled rice (PR) has a low risk of disease, but little is known about the contribution of PR to the prevention of hyperlipidemia. The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study. Male C57BL/6J mice were fed with a normal diet, high-fat diet (HFD) containing refined rice (HFDRR) or PR (HFDPR). It was found that PR intervention improved lipid accumulation in mice. Transcriptomic data analysis revealed that 27 genes were up-regulated (mostly involved in lipid breakdown) and 86 genes were down-regulated (mostly involved in inflammatory responses) in the HFDPR group compared to the HFDRR group. And 15 differentially expressed genes (DEGs) were validated by quantitative real-time PCR (RT-qPCR), while protein interaction network showed that protein tyrosine phosphatase receptor type C (PTPRC) has a central role. The gut microbiota of mice was also altered after different dietary treatments, with higher ratio of Firmicutes and Bacteroidetes, increased abundances of Ruminococcaceae, Lachnospiraceae, Christensenellaceae, Porphyromonadaceae, Rikenellaceae and Prevotellaceae, and decreased abundances of Lactobacillaceae, Peptostreptococcaceae, Erysipelotrichaceae and Actinobacteria in the HFDRR group. In addition, it was observed that PPAR signaling pathway may act as a bridge between DEGs and differential gut microbiota. These results suggested that PR can prevent hyperlipidemia by modulating liver genes and gut microbiota. |
| format | Article |
| id | doaj-art-2da75ac14b664da7925899203af4e518 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-2da75ac14b664da7925899203af4e5182025-01-10T06:54:23ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-05-011331422143810.26599/FSHW.2022.9250120Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in miceXiuxiu Wu0Tianyi Guo1Biao Li2Shuai Han3Zuomin Hu4Yi Luo5Dandan Qin6Yaping Zhou7Feijun Luo8Qinlu Lin9National Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaCollege of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaDepartment of Clinical Medicine, Medical College of Xiangya, Central South University, Changsha 410008, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaNational Research Center of Rice Deep Process and Byproducts, Hunan Provincial Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Provincial Key Laboratory of Forestry Edible Resources Safety and Processing, Central South University of Forestry and Technology, Changsha 410004, ChinaDietary parboiled rice (PR) has a low risk of disease, but little is known about the contribution of PR to the prevention of hyperlipidemia. The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study. Male C57BL/6J mice were fed with a normal diet, high-fat diet (HFD) containing refined rice (HFDRR) or PR (HFDPR). It was found that PR intervention improved lipid accumulation in mice. Transcriptomic data analysis revealed that 27 genes were up-regulated (mostly involved in lipid breakdown) and 86 genes were down-regulated (mostly involved in inflammatory responses) in the HFDPR group compared to the HFDRR group. And 15 differentially expressed genes (DEGs) were validated by quantitative real-time PCR (RT-qPCR), while protein interaction network showed that protein tyrosine phosphatase receptor type C (PTPRC) has a central role. The gut microbiota of mice was also altered after different dietary treatments, with higher ratio of Firmicutes and Bacteroidetes, increased abundances of Ruminococcaceae, Lachnospiraceae, Christensenellaceae, Porphyromonadaceae, Rikenellaceae and Prevotellaceae, and decreased abundances of Lactobacillaceae, Peptostreptococcaceae, Erysipelotrichaceae and Actinobacteria in the HFDRR group. In addition, it was observed that PPAR signaling pathway may act as a bridge between DEGs and differential gut microbiota. These results suggested that PR can prevent hyperlipidemia by modulating liver genes and gut microbiota.https://www.sciopen.com/article/10.26599/FSHW.2022.9250120parboiled riceblood lipidstranscriptomegut microbiotapparsignaling pathway |
| spellingShingle | Xiuxiu Wu Tianyi Guo Biao Li Shuai Han Zuomin Hu Yi Luo Dandan Qin Yaping Zhou Feijun Luo Qinlu Lin Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice Food Science and Human Wellness parboiled rice blood lipids transcriptome gut microbiota ppar signaling pathway |
| title | Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice |
| title_full | Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice |
| title_fullStr | Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice |
| title_full_unstemmed | Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice |
| title_short | Parboiled rice supplementation alleviates high-fat diet-induced hyperlipidemia by regulating genes and gut microbiota in mice |
| title_sort | parboiled rice supplementation alleviates high fat diet induced hyperlipidemia by regulating genes and gut microbiota in mice |
| topic | parboiled rice blood lipids transcriptome gut microbiota ppar signaling pathway |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250120 |
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